Dr. Pratibha Rani Deep


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Course Name: Microbiology


Code(Credit) : CUTM1436 (3-1-0)


Course Objectives

To know various Culture media and their applications and also understand various physical and chemical means of sterilization .

Master aseptic techniques and be able to perform routine culture handling tasks safely and effectively.

To know the various Physical and Chemical growth requirements of microbes and get equipped with various methods of microbes culture techniques and their role in various industry.

Learning Outcomes

At the time of completion of the programme the student will have developed extensive knowledge in various areas of Microbiology.

They will be able to explain vaccine strategies and mechanisms of antiviral drugs and interferons.

Can know how viruses can be used as tools to study biological processes, as cloning vectors and for gene transfer.

Understand the interactions between viruses, bacteria and the host immune system.

The student will be instilled with values of professional ethics and be made ready to contribute to society as responsible individuals.

Course Syllabus


Bacteria and virus

Classification, taxonomy, cataloguing virus to ICTV and ICNV

Structural and genetic diversity of viruses
Transmission and Replication
Prions, Viriods, Anti viral agents, and Vaccines
Bacterial Classification ( phenetic, genetic and phyllogenetic)
Bergeys manual of systematic bacteriology
Classification, Identification and Culturing Technique of cyanobacteria
Industrial Application, Cyanotoxins.

Practice 1: Preparation of bacterial smear and staining – Gram’s, Acid-fast, Staining of bacterial spores flagella, capsule, spirochaetes

Practice 2:Isolation, purification, identification and biomass production of cyanobacteria


Module 2

Microbial Physiology and Metabolism

Growth Kinetics, Growth cycle, Logistic growth equation,
Measurement and growth monitoring in culture, Factor affecting growth.

Photosynthetic pigments, Paths of carbon and electron in bacterial photosynthesis.
Fermentation, Respiratory metabolism, Embden-Meyerhoff pathway, Entner-Doudroff pathway, Pasteur Effect.

Practice 3: Methods for measurement of bacterial growth by haemocytometer and spectrophotometer


Module 3

Environmental Microbiology & Wastewater Management:

Microbes and quality of environment,  Biotransformations, Microbes in waste water management; Microbial degradation of pesticides, toxic chemicals, oil; Bioleaching, bioremediation.


Module 4

Agricultural and food Microbiology

Agriculturally important microorganisms, Mycorrhizae, Microbial mineralization, Microbial toxins, Biological control.

Microbial toxins produced in food items, Probiotics and preBiotics, Methods of food preservation, Microbiological legal standards of selected food and milk products.

Practice 4: Qualitative analysis of Milk and milk products; Microbiological analysis of food products; Detection of bacteria in milk by Standard plate count


Module 5
Medical Microbiology:

Host pathogen interactions,  Pathogenicity of bacteria invasiveness and toxigenicity, Constitutive and inducible host defence mechanism, Important diseases caused by bacteria, protozoa, virus.

Antibiotics: Definition, phenomenon of antibiotics, Chemical and biochemical modification of antibiotic structures, assay and Mode of action, Biochemical mechanisms of resistance development, Multiple-drug resistance.

Practice 5: Antibiotic sensitivity test disc preparation; Antibiotic sensitivity test – Kirby – Bauer, Stoke’s; MIC determination by filter paper disc assay


Module 6

Industrial Microbiology

Cyanobacterial Biotechnology:

Application as nutraceuticals, pharmaceuticals, cosmetic, biofertilizer; application as biofuel, CO2 sequestration and pollution control, Mass cultivation, Single cell protein.

Microbial enzymes: Sources, Large scale production, Recovery,
Microbial enzymes of industrial interest, Novel medicines from microbes, Biotechnologial application of Microbial enzyme, Use of Microbes in Biotechnology.

Bioprocess technology and Engineering:

Culture media (types, Different culturing Technique, Media formulation, Preservation of Microbes, Fermenter design and growth processes, Bioreactors, and Membrane Bio reactors,
Analysis of different bioreactors, stability of microbial reactors, specialized bioreactors. Isolation, preservation, and Maintenance of Industrial Microorganisms.

Practice 6: Preparation of different microbial culture media


Module 7

Microbial genetics

Lytic and Lysogenic cycle, Conjugation, Transduction, Recombination
Genetic regulation: Operon concept (lac,trp ara), Genetic mapping: Genome mapping of E. coli, QTL Mapping.
Molecular markers in genome analysis, RAPD, RFLP,AFLP, FISH and GISH.



Text Books:

Prescott, L. M., Harley, J. P. and Klen, D. A. (1999). Microbiology, 7th Ed., McGraw-Hill, New York.

Pelczar, Jr., M. J., Chan E.C.S. and Krieg, N. R. (2005). Microbiology, 5th Ed, Tata McGraw-Hill, New Delhi.

Alexopoulus, C. J., Mims, C. W. and Blackwel, M. (1996). Introductory Mycology, John Wiley, New York.

Kumar, H. D. (1988). Introductory Phycology. East-West Press, New Delhi.

Maloy, S. R., Cronan, J. E. Jr. and Freifielder, D. (2008). Microbial Genetics, 2nd Ed. Norosa, New Delhi.


Reference Books

Mehrotra, R. S. and Aneja, R. S. (1998). An Introduction to Mycology, New Age International, New Delhi

Agrios, G. N. (2005). Plant Pathology, 5th Ed, Elsevier Academic press, USA

Session Plan


Bacteria and virus

Classification, taxonomy, cataloguing virus to ICTV and ICNV



Session 6

Session 9 (Practice 2)

Session 19 (Practice 5)

Qualitative analysis of Milk and milk products; Microbiological analysis of food products; Detection of bacteria in milk by Standard plate count





Session 23 (Practice 6)

Antibiotic sensitivity test disc preparation; Antibiotic sensitivity test – Kirby – Bauer, Stoke’s ; MIC determination by filter paper disc assay





Session 27

Microbial enzymes:Sources, Large scale production, Recovery,


Large scale production,


Session 32

Analysis of different bioreactors, stability of microbial reactors, specialized bioreactors

Analysis of different bioreactors,

specialized bioreactors


Case Studies

Case Studies

Our Main Teachers

Dr. Pratibha Rani Deep

Assisstant Professor in Botany, School of Applied Science, ( Bolangir Campus)

Dr. Pratibha R. Deep works as an Assistant Professor, Department of Botany, at the Centurion University of Technology and Management, Bolangir Campus, Odisha India. She has received her M.Sc. (Botany) from Gangadhar Meher College (presently University), Sambalpur, India. She qualified GATE twice 2007 and 2012. She did her MTech in Applied Botany from IIT Kharagpur, […]