Still no participant
Still no reviews
This subject deals with various advanced analytical instrumental techniques for
identification, characterization and quantification of drugs. Instruments dealt are
NMR, Mass spectrometer, IR, HPLC, GC etc.
After completion of course student is able to know,
Chemicals and Excipients
The analysis of various drugs in single and combination dosage forms
Theoretical and practical skills of the instruments
1. a. UV-Visible spectroscopy: Introduction, Theory, Laws,
Instrumentation associated with UV-Visible spectroscopy, Choice of solvents and solvent effect and Applications of UV-Visible spectroscopy.
b. IR spectroscopy: Theory, Modes of Molecular vibrations,
Sample handling, Instrumentation of Dispersive and Fourier - Transform IR Spectrometer, Factors affecting vibrational frequencies and Applications of IR spectroscopy
c. Spectroflourimetry: Theory of Fluorescence, Factors affecting fluorescence, Quenchers, Instrumentation and Applications of fluorescence spectrophotometer.
d. Flame emission spectroscopy and Atomic absorption
spectroscopy: Principle, Instrumentation, Interferences and Applications.
2. NMR spectroscopy: Quantum numbers and their role in NMR,
Principle, Instrumentation, Solvent requirement in NMR, Relaxation process, NMR signals in various compounds,
Chemical shift, Factors influencing chemical shift, Spin-Spin coupling, Coupling constant, Nuclear magnetic double resonance, Brief outline of principles of FT-NMR and 13C NMR. Applications of NMR spectroscopy.
3. Mass Spectroscopy: Principle, Theory, Instrumentation of Mass
Spectroscopy, Different types of ionization like electron impact, chemical, field, FAB and MALDI, APCI, ESI, APPI Analyzers of Quadrupole and Time of Flight, Mass fragmentation and its rules, Meta stable ions, Isotopic peaks and Applications of Mass
4. Chromatography: Principle, apparatus, instrumentation, chromatographic parameters, factors affecting resolution and applications of the following:
a) Paper chromatography b) Thin Layer chromatography
c) Ion exchange chromatography d) Column chromatography
e) Gas chromatography f) High Performance Liquid
g) Affinity chromatography
5 a. Electrophoresis: Principle, Instrumentation, Working
conditions, factors affecting separation and applications of the
a) Paper electrophoresis b) Gel electrophoresis c) Capillary
electrophoresis d) Zone electrophoresis e) Moving boundary
electrophoresis f) Iso electric focusing
b. X ray Crystallography: Production of X rays, Different X ray
diffraction methods, Bragg‘s law, Rotating crystal technique, X
ray powder technique, Types of crystals and applications of Xray
6. Immunological assays: RIA (Radio immuno assay), ELISA,
1. Spectrometric Identification of Organic compounds - Robert M Silverstein,
Sixth edition, John Wiley & Sons, 2004.
2. Principles of Instrumental Analysis - Doglas A Skoog, F. James Holler,
Timothy A. Nieman, 5th edition, Eastern press, Bangalore, 1998.
3. Instrumental methods of analysis – Willards, 7th edition, CBS publishers.
4. Practical Pharmaceutical Chemistry – Beckett and Stenlake, Vol II, 4th
edition, CBS Publishers, New Delhi, 1997.
5. Organic Spectroscopy - William Kemp, 3rd edition, ELBS, 1991.
6. Quantitative Analysis of Drugs in Pharmaceutical formulation - P D Sethi, 3rd
Edition, CBS Publishers, New Delhi, 1997.
7. Pharmaceutical Analysis- Modern methods – Part B - J W Munson, Volume
11, Marcel Dekker Series
Session 1: Concept of UV visible spectroscopy, theory and Laws of UV spectroscopy
Session 2: Instrumentation, effect of Solvents and App;ications of UV
Session 3: Introduction to IR spectroscopy
Infrared spectroscopy _ HiQ (Introduction, theory)
Session 4: Instrumentation of IR spectroscopy
Session 5: Types of vibrations to IR spectroscopy and its Applications
Session 6: Spectroflourimetry: Theory of Fluorescence, Factors
affecting fluorescence, Quenchers, Instrumentation
Session 7: Flame emission spectroscopy and Atomic absorption
spectroscopy: Principle, Instrumentation and Applications
flame phtometry (Principle, theory)
Session 8: Flame Photometry Instrumentation and Applications
Session 9: NMR spectroscopy: Quantum numbers and their role in NMR,
Principle, Instrumentation, Solvent requirement in NMR,
Relaxation process, Applications