Nano-pharmaceuticals and Bio-Medical science

Teacher

Dr. Srikanta Sahu

Category

Core Courses

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Course Name : Nano-pharmaceuticals and Bio-Medical science

Code(Credit) : CUTM1422(3-0-1)

Course Objectives

  • To obtain an hands-on experience in converting a small novel idea / technique into a working model / prototype involving multi-disciplinary skills and / or knowledge and working in at team.

Course Outcome

COs Course outcomes Mapping COs with POs (High-3, Medium-2, Low-1)
CO1 Able to gain Knowledge on implementing chemistry, biology and nano-science to solve the biomedical problems. PO1 (3)
CO2 Research and problem Solving PO2(3), PO3(2)
CO3 Design and Development PO3(3)
CO6 Individual and Team work PO6(3)
  • To conceptualize a novel idea / technique into a product or paper

  • To think in terms of multi-disciplinary environment

  • To understand the management techniques of implementing a project

  • To take on the challenges of teamwork, prepare a presentation in a professional manner, and document all aspects of design work

  • To bridge the gap between industry and academia and they can pursue their dream in either directions.

Course Syllabus

Module I  (6) hours

Nanotechnology in Food and pharmaceutical industry: Nano particle based drug delivery systems, Regenerative medicine, nano-immuno conjugates, Bio-availability and delivery of nutraceuticals and functional foods using nanotechnology, Polymer-based nano-composites for food packaging, nano-composites for food packaging, Toxicity and environmental risks of nano-materials

Assignment 1: Nano-composites for food packaging, Toxicity and environmental risks of nano-materials

Module II  (6) hours

Nanotechnology in Biomedical and Pharmaceutical Industry: Tissue engineering/regenerative medicine, Nano-robotics in surgery, Nano-tools for early detection diseases, Nano-medicine for cancer treatment

Assignment 2 : Tissue engineering/regenerative medicine

Module III  (6) hours

Nanotechnology in Agriculture: Introduction of Nanotechnology in agriculture, Potential of nano-fertilizers, Precision farming, smart delivery system, Insecticides using nanotechnology

Assignment 3 : Smart delivery system

Module IV  (6) hours

Molecular Cell Biology: Cell- Structure & Function of Cell Membrane, Different cell types and their Functions, Sub-cellular Organelles and their Functions, Nucleotide- Structure and Functions of DNA & RNA. Biologically important nucleotide, protein synthesis, unnatural amino acid, Mechanistic understanding of various diseases and target identification for early detection

Assignment 4: Cell culture techniques and Visualizing cell organelles using fluorescent probes

Module V  (6) hours

Optical and non-optical probes: Introduction to Fluorescence, Designing of Organic and in-organic Fluorophores and study of photo-physical properties, Synthesis of Nano and bio-probe, study of sensing properties, Chemistry of Non-optical probe: PET and MRI probe

Assignment 5 : Uderstand the concept of fluorescence using laser light and Synthesis of Fluorescein dye

Module VI  (6) hours

Protein Labeling and bio-medical applications: Affinity based protein labeling, Labeling of protein through unnatural amino acid, Diagnostic Probe for early detection of diseases and bio-analytes

Assignment 6: Fluorescent Labeling of proteins for Live Cell Imaging

Module VII (8) hours

Target Identification and Drug designing: High-Throughput Screenings, Affinity matrix approaches: On-bead affinity matrix, Biotin tags in affinity matrix, Fluorescent tags in affinity  matrix, Photo-affinity tags in affinity matrix, Drug western approaches, Three-hybrid system approaches, Three-hybrid system approaches:   Yeast three-hybrid systems, Mammalian three-hybrid systems, Phage display approaches, mRNA display approaches, Protein micro-array approaches, Drug affinity responsive target stability (DARTS)

Assignment 7 : A practical approach to antibody labeling

Books Recommended:

  • Berg, J.M., Tymoczko, J. L. and Stryer, L. (2006) Biochemistry. VIth Edition. W.H. Freeman and Co.
  • Nelson, D. L., Cox, M. M. and Lehninger, A. L. (2009) Principles of Biochemistry. IV Edition. W.H. Freeman and Co.
  • Murray, R. K., Granner, D. K., Mayes, P. A. and Rodwell, V.W. (2009) Harper’s Illustrated Biochemistry. XXVIII edition. Lange Medical Books/ McGraw-Hill.

Session Plan

Session 1  (1 hour) Module-I

Session 2  (1 hour)

Session 3  (1 hour)

Session 4  (1 hour)

Polymer-based nano-composites for food packaging

https://www.youtube.com/watch?v=K__Hi-AmEVg

Reference:

https://www.youtube.com/watch?v=Ktb5jhrLTWk

Session 5  (2 hour)

Assignment 1: Nano-composites for food packaging, Toxicity and environmental risks of nano-materials

https://www.youtube.com/watch?v=Ktb5jhrLTWk

Session 6  (1 hour), Module II

Session 7  (1 hour), Module II

Session 8  (1 hour), Module II

Session 9  (1 hour), Module II

Session 10  (1 hour), Module II

Session 11  (1 hour), Module III

Introduction of Nanotechnology in agriculture

https://www.youtube.com/watch?v=hdDBvC7kop8

Reference:

https://www.youtube.com/watch?v=vDDQAkCMyl8

Session 12  (1 hour), Module III

Session 13  (1 hour), Module III

Session 14  (1 hour), Module III

Insecticides using nanotechnology

Session 15  (2 hour), Module III

Assignment 3 : Smart delivery system

https://www.youtube.com/watch?v=VvZAEuxqdY4

Session 16  (1 hour), Module IV

Molecular Cell Biology, Cell- Structure & Function of Cell Membrane, Different cell types and their Functions

https://www.youtube.com/watch?v=URUJD5NEXC8

Session 17  (1 hour), Module IV

Sub-cellular Organelles and their Functions

https://www.youtube.com/watch?v=ZyWYID2cTK0

Session 18  (1 hour), Module IV

Mechanistic understanding of various diseases and target identification for early detection

https://www.coursera.org/learn/cancer/lecture/XsshR/incidence-and-etiology-of-cancer

Reference:

https://www.youtube.com/watch?v=aDS_LqQf1mk

Session 19  (1 hour), Module IV

Nucleotide- Structure and Functions of DNA & RNA. Biologically important nucleotide, protein synthesis, unnatural amino acid

https://www.youtube.com/watch?v=gG7uCskUOrA&list=RDQM1TXXAdELXtU&start_radio=1

http://biology.kenyon.edu/courses/biol63/watson_06.pdf

Session 20  (2 hour), Module IV

Session 21  (1 hour), Module V

Optical and non-optical probes, Introduction to Fluorescence

https://www.youtube.com/watch?v=W9p6Y8IW89A

Reference:

https://www.youtube.com/watch?v=3ev_TnFE2to

Session 22  (1 hour), Module V

Designing of Organic and in-organic Fluorophores and study of photo-physical properties

https://www.youtube.com/watch?v=Ok__iaEx_Hc

Reference:

https://www.youtube.com/watch?v=gSGq8gOLXwY

Session 23  (1 hour), Module V

Session 24  (1 hour), Module V

Session 25  (2 hour), Module V

Assignment 5 : Uderstand the concept of fluorescence using laser light and Synthesis of Fluorescein dye

References:

https://www.youtube.com/watch?v=CcssdJf0pKQ

https://www.youtube.com/watch?v=_-ZKef9nyLA

https://www.youtube.com/watch?v=ihssISvNL3o

Session 26  (1 hour), Module VI

Protein Labeling strategy and development of probe for bio-medical application, Bio-Orthogonal chemistry for Protein labeling

References:

https://www.youtube.com/watch?v=xOmwI-q_xPA

https://sci-hub.tw/10.1021/acs.chemrev.6b00084

Session 27  (1 hour), Module VI

Session 28  (1 hour), Module VI

Labeling of protein through unnatural amino acid

https://sci-hub.tw/10.1021/cr400355w

Session 29  (1 hour), Module VI

Session 30  (2 hour), Module VI

Assignment 6: Fluorescent Labeling of proteins for Live Cell Imaging

Session 31  (1 hour), Module VII

Target Identification and Drug designing, High-Throughput Screenings

Session 32  (1 hour), Module VII

Affinity matrix approaches: On-bead affinity matrix, Biotin tags in affinity matrix

https://sci-hub.tw/10.4051/ibc.2011.3.1.0003

Session 33  (1 hour), Module VII

Fluorescent tags in affinity matrix, Photo-affinity tags in affinity matrix

References:

https://www.youtube.com/watch?v=JjbOBbr4q0s

https://www.youtube.com/watch?v=7O0V3ocMiAQ

Session 34  (1 hour), Module VII

Drug western approaches, Three-hybrid system approaches

https://sci-hub.tw/10.4051/ibc.2011.3.1.0003

Session 35  (1 hour), Module VII

Three-hybrid system approaches: Yeast three-hybrid systems, Mammalian three-hybrid systems

References:

https://www.youtube.com/watch?v=JjbOBbr4q0s

https://sci-hub.tw/10.4051/ibc.2011.3.1.0003

Session 36  (1 hour), Module VII

Phage display approaches, mRNA display approaches, Protein micro-array approaches, Drug affinity responsive target stability (DARTS)

References:

https://www.youtube.com/watch?v=JjbOBbr4q0s

https://sci-hub.tw/10.4051/ibc.2011.3.1.0003

https://www.youtube.com/watch?v=7O0V3ocMiAQ

Session 37  (1 hour), Module VII

Phage display approaches, mRNA display approaches, Protein micro-array approaches, Drug affinity responsive target stability (DARTS)

References:

https://www.youtube.com/watch?v=JjbOBbr4q0s

https://sci-hub.tw/10.4051/ibc.2011.3.1.0003

https://www.youtube.com/watch?v=7O0V3ocMiAQ

Session 38  (2 hour), Module VII

Case Studies

Case Studies

Our Main Teachers

Srikanta Sahu completed his Ph.D.  from IIT Delhi in Bio-organic chemistry under the supervision of Prof. V. Haridas. After his PhD, he moved for postdoctoral work at National University of Singapore (NUS) in the field of Bio-medical science and chemical biology under the guidance of Prof. Young Tae Chang. Later, he joined as a core […]