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Introduction
Ocular Microbiology is the study of microorganisms (bacteria, viruses, fungi, and parasites) that infect the eye and its surrounding structures. These microbes can cause conditions such as conjunctivitis, keratitis, endophthalmitis, and uveitis. Understanding the type of pathogen and its mode of infection is essential for effective diagnosis, treatment, and prevention of vision-threatening diseases.
Course Objective:
Course Content
o Types of Microorganisms
o Physiology of Microorganisms
o Nutrition, Enzymes, Metabolism, and Energy
o Microbial Growth
o Sterilization and Disinfection in the Laboratory
o Control of Microbial Growth (Antimicrobial Methods and Chemotherapy)
o Microbes vs. Humans: Infection Development, Disease Process, Pathogenicity, and Virulence
o Pathophysiology of Ocular Angiogenesis
o Ocular Infections
o Pathology of Cornea and Conjunctiva
o Pathology of Uvea and Glaucoma
o Pathology of Retina
o Staphylococcus aureus
o Staphylococcus epidermis
o Streptococcus
o Propionic Bacterium
o Actinomyces
o Nocardia
o Pseudomonas
o Haemophilus
o Brucella
o Neisseria
o Moraxella
o Pathology of Retina in Systemic Diseases/Disorders
o Pathology of Eyelids and Adnexa
o Pathology of Orbital Space-Occupying Lesions
o Pathology of the Optic Nerve
o Retinoblastoma
o Pathology of Lens
o Treponema
o Leptospiraceae
o Classification of Viruses in Ocular Disease
o Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV
o Yeasts
o Filamentous and Dimorphic Fungi
o Intracellular Parasites (Chlamydia)
o Protozoa (Toxoplasmosis, Acanthamoeba)
o Helminths (Toxocariasis, Filariasis, Onchocerciasis, Trematodes)
1) Microbiology: M J Pelczaretal., 1999
1) BURTON G.R.W: Microbiology for the Health Sciences, St. Louis, J.P. Lippincott Co.,3rdEdn.,1988.
2) Pathology: CORTON KUMAR AND ROBINS (V EDITION) Pathological Basis of the Disease, 2004.
3) Pathology of the eye & orbit: K S Ratnagar, 1997
4) MACKIE &Mc CARTNEY Practical Medical Microbiology
5) SYDNEY M. FINEGOLD & ELLEN JO BARON: Diagnostic Microbiology (DM)
6) Sherris Medical Microbiology- Editors Kenneth J Ryan /C.George Ray :An Introduction to InfectiousDiseases 4th Edition 2003
7) CORTON KUMAR AND ROBINS (IV EDITION) : Pathological Basis of the Disease, 1994
8) S R Lakhani Susan AD & Caroline JF: Basic Pathology: An introduction to the mechanism of disease, 1993.
Session 1
Introduction to Microbiology and Microbial Growth
Instructor’s PPT https://docs.google.com/presentation/d/1COKTRfyu2pazpLo83IsU6JnmPSKq06iI/edit?slide=id.p1#slide=id.p1
Session 2
Physiology of Microorganisms
Instructor’s PPT https://docs.google.com/presentation/d/1v7GDDBh4u4EDHGq7vDuKuS8LJtG5AbQu/edit?slide=id.p1#slide=id.p1
Session 3, 4, 5
Nutrition, Enzymes, Metabolism, and Energy
Instructor’s PPT https://docs.google.com/presentation/d/1v7GDDBh4u4EDHGq7vDuKuS8LJtG5AbQu/edit?slide=id.p1#slide=id.p1
Session 6, 7
Microbial Growth
Instructor’s PPT https://docs.google.com/presentation/d/1v7GDDBh4u4EDHGq7vDuKuS8LJtG5AbQu/edit?slide=id.p1#slide=id.p1
Session 8,9
Sterilization and Disinfection in the Laboratory
Instructor’s PPT https://docs.google.com/presentation/d/1eyTBWYftu5cv8dDP2ZnUdpIKkSYw8TzM/edit?slide=id.p1#slide=id.p1
Session 10,11
Control of Microbial Growth (Antimicrobial Methods and Chemotherapy)
Instructor’s PPT https://docs.google.com/presentation/d/1v7GDDBh4u4EDHGq7vDuKuS8LJtG5AbQu/edit?slide=id.p1#slide=id.p1
Session 12,13
Pathophysiology of Ocular Angiogenesis
Instructor’s PPThttps://drive.google.com/drive/u/0/folders/1QUSDfwkZddYyuRhF4UlONU2ig4BSVs_D
Session 14, 15
Ocular Infection
Instructor’s PPT https://docs.google.com/presentation/d/1J01bxr4v1_8fwHhbeyUIlOPIwkNh0heS/edit?slide=id.p1#slide=id.p1
Session 16,17
Pathology of Cornea and Conjunctiva
Instructor’s PPT https://docs.google.com/presentation/d/15M6DPxGzkBWimIuFY8P1ogSwyzlkaTMG/edit?slide=id.p1#slide=id.p1
Session 18,19
Pathology of Uvea and Glaucoma
Instructor’s PPT https://docs.google.com/presentation/d/1dElGwssUNiq-vdLSFyZzMiTpUO_kI1hS/edit?slide=id.p1#slide=id.p1
Session 20
Pathology of Uvea and Glaucoma
Instructor’s PPT https://docs.google.com/presentation/d/1KlDZY7JZzZd3skaggMYfb3wcr33FwUxe/edit?slide=id.p1#slide=id.p1
Session 21 , 22
Staphylococcus aureus
Instructor’s PPT https://docs.google.com/presentation/d/1hwr-SGjbeJ58zszuuwZ8F5_qjZRgghd1/edit?slide=id.p1#slide=id.p1
Session 23,24
Staphylococcus epidermis
Instructor’s PPT https://docs.google.com/presentation/d/1EqHoJxAXco1FuWj-9PYR8ixPoaRaPCT7/edit?slide=id.p1#slide=id.p1
Session 25,26
Propionic Bacterium
Instructor’s PPThttps://drive.google.com/drive/u/0/folders/1QUSDfwkZddYyuRhF4UlONU2ig4BSVs_D
Session 27,28
Actinomyces
Instructor’s PPT https://drive.google.com/drive/u/0/folders/1QUSDfwkZddYyuRhF4UlONU2ig4BSVs_D
Session 29,30
Nocardia
Instructor’s PPT https://drive.google.com/drive/u/0/folders/1QUSDfwkZddYyuRhF4UlONU2ig4BSVs_D
session 31
Pseudomonas, Haemophilus
Instructor’s PPT https://docs.google.com/presentation/d/1fTf54erSn02ZrKS0OIWjRHqvekRlIC8G/edit?slide=id.p1#slide=id.p1
Session 33,34
Brucella, Neisseria
Instructor’s PPThttps://docs.google.com/presentation/d/1ECZT7clT9dmwRws_QjCXRATovRRP3yxH/edit?slide=id.p1#slide=id.p1
Session 35,36
Pathology of Retina in Systemic Diseases/Disorders
Instructor’s PPT https://docs.google.com/presentation/d/1TmvjyVOS72EGfOzOhnc5ytQeCMjTeUAl/edit?slide=id.p1#slide=id.p
Session 37,38
Pathology of Eyelids and Adnexa
Instructor’s PPT https://docs.google.com/presentation/d/1YKLQ20vtK4YRN_gFj98BnA4VTUJgwt_r/edit?slide=id.p1#slide=id.p1
Session 39,40
Pathology of Orbital Space-Occupying Lesions
Instructor’s PPT https://docs.google.com/presentation/d/1nrWYM8d-MRd21NNozDLLDKs_PUUky551/edit?slide=id.p1#slide=id.p1
Session 41,42
Pathology of Orbital Space-Occupying Lesions
Instructor’s PPT https://docs.google.com/presentation/d/1nrWYM8d-MRd21NNozDLLDKs_PUUky551/edit?slide=id.p1#slide=id.p1
Session 43,44
Helminths (Toxocariasis, Filariasis, Onchocerciasis, Trematodes)
Instructor’s PPT https://docs.google.com/presentation/d/1ZaDgGs-f15Wkz534EC-cS_VDm35zyWg5/edit?slide=id.p1#slide=id.p
OCULAR MICROBIOLOGY and PATHOLOGY
Module-1: 8hrs
Introduction to Microbiology :Types of Microorganisms, Physiology of Microorganisms –Nutrition, Enzymes, Metabolism and energy, Microbial Growth, Sterilization and disinfection in the laboratory ,Control of Microbial Growth – Antimicrobial methods and Chemotherapy, Microbes versus Humans- The development of Infection, the disease process, pathogenicity and virulence.
Module-2: 8hrs
General Pathology : Principles ,Pathophysiology of Ocular Angiogenesis , Ocular Infections,Pathology of cornea and Conjunctiva , Pathology of Uvea, Glaucoma Retina
Module-3 : 8hrs
Ocular Bacteriology-Gram positive,(Staphylococcus auras, Staphylococcus epidermis,Streptococcus, propionic bacterium, actinomycesNocardia) Bacteria including acid fast bacilli, Mycobacterium tuberculosis, Myobacteriumleprae)
Module-4: 6hrs
Ocular Bacteriology - Gram negative Bacteria (pseudomonas, haemophiilus, Brucella, Neisseria, Moraxella) .
Module-5 6hrs
Pathology of retina in systemic disease/disorders ,Pathology of eyelids and adnexa, Pathology of orbital space occupying lesions
Module-6 8hrs
Spirochetes (Treponema, Leptospiraceae),Virology: Classification of Viruses in Ocular Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. ,
Fungi : Yeasts, Filamentous, Dimorphic ,Intracellular parasites - Chlamydia, Protozoa (Taxoplasmosis, Acanthamoeba,) Helminths ( Toxocariasis , Filariasis , Onchocerciasis , Trematodes )
Module-7 2hrs
Pathology of the optic nerve, Retinoblastoma,Pathology of Lens .
Physiology of Microorganisms –Nutrition, Enzymes, Metabolism and energy:
Sterilization and disinfection in the laboratory :
Control of Microbial Growth – Antimicrobial methods and Chemotherapy:
Microbes versus Humans- The development of Infection, the disease process, pathogenicity and virulence.
General Pathology :
https://www.youtube.com/watch?v=S2nti5bLtiM&list=PLiuke9_ROlvpOiXKqtWVjMhs8V2XeRZGk
Principles:
Pathophysiology of Ocular Angiogenesis:
Ocular Infections:Pathology of cornea and Conjunctiva :
Pathology of Uvea:
Ocular Bacteriology-Gram positive:
Staphylococcus auras, Staphylococcus epidermis:
Session 14 : 3hrs
Streptococcus:
https://www.youtube.com/watch?v=HAtu6uNUZyY
propionic bacterium:
https://www.youtube.com/watch?v=-lfKbjDctF0
actinomycesNocardia:
Session 15 :
Bacteria including acid fast bacilli: Mycobacterium tuberculosis
Myobacteriumleprae:
Ocular Bacteriology - Gram negative Bacteria
Brucella
https://www.youtube.com/watch?v=pixZUrdn-wY
Neisseria
https://www.youtube.com/watch?v=OayQEFV0vJA
Moraxella.
https://www.youtube.com/watch?v=WrRWI-grMXc
Pathology of retina in systemic disease/disorders
Pathology of eyelids and adnexa
Pathology of orbital space occupying lesions
Spirochetes (Treponema, Leptospiraceae),
Virology: Classification of Viruses in Ocular Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. ,
https://www.youtube.com/watch?v=Rpj0emEGShQ&list=PL5lHe_3BHEka0875fY_eS_YS-wcs89uZr
Fungi : Yeasts, Filamentous, Dimorphic ,Intracellular parasites - Chlamydia,
Protozoa (Taxoplasmosis, Acanthamoeba,) Helminths ( Toxocariasis , Filariasis , Onchocerciasis , Trematodes )
Pathology of the optic nerve,
Retinoblastoma,
Pathology of Lens