OCULAR MICROBIOLOGY & PATHOLOGY. CUTM -1789

Teacher

Syamala Alana Teja

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COURSE OBJECTIVES

  • This course covers the basic biological characteristics of pathogenic organisms
  • Biochemical
  • Pathogenic characteristics of the various microorganisms

COURSE OUTCOMES

  • T prepare the students to gain essential knowledge about the characteristics of bacteria ,viruses, fungi and parasites .
  • To acquire knowledge of the principle of sterilisation and disinfection in hospital and ophthalmic practice
  • To understand the pathogenesis of the disease caused by the organisms in the human body with particular reference to the eye infections
  • To understand the basic principles of diagnostic ocular Microbiology

course syllabus :

Module-1

Introduction to Microbiology :Types of Microorganisms, Physiology of Microorganisms –
Nutrition, Enzymes, Metabolism and energy, Microbial Growth ,Sterilization and disinfection
in the laboratory ,Control of Microbial Growth – Antimicrobial methods and Chemotherapy,
Microbes versus Humans- The development of Infection, the disease process, pathogenicity
and virulence.
Practice: Handling of microscope

Module-2

General Pathology : Principles ,Pathophysiology of Ocular Angiogenesis , Ocular Infections,
Pathology of cornea and Conjunctiva , Pathology of Uvea, Glaucoma Retina

Module-3

Ocular Bacteriology-Gram positive,(Staphylococcus auras, Staphylococcus epidermis,
Streptococcus, propionic bacterium, actinomycesNocardia) Bacteria including acid fast bacilli,

Mycobacterium tuberculosis, Mycobacterium Leprae)

Practice: Gram staining procedures

Module-4

Ocular Bacteriology - Gram negative Bacteria (pseudomonas, haemophiilus, Brucella,
Neisseria, Moraxella) .

Module-5

Pathology of retina in systemic disease/disorders , Pathology of eyelids and adnexa ,Pathology
of orbital space occupying lesions
Practice: Slit-lamp Examination

Module-6

Spirochetes (Treponema, Leptospiraceae),Virology: Classification of Viruses in Ocular
Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. ,
Fungi : Yeasts, Filamentous, Dimorphic ,Intracellular parasites - Chlamydia, Protozoa
(Taxoplasmosis, Acanthamoeba,) Helminths ( Toxocariasis , Filariasis , Onchocerciasis ,
Trematodes )
Module-7

Pathology of the optic nerve, Retinoblastoma,Pathology of Lens .

 

Text Books:

1. Microbiology : M J Pelczaretal., 1999
2. BURTON G.R.W : Microbiology for the Health Sciences, St. Louis, J.P.
Lippincott Co.,3 rd Edn.,1988..
3. Pathology : CORTON KUMAR AND ROBINS (V EDITION) Pathological Basis
of the Disease, 2004.
4. Pathology of the eye & orbit : K S Ratnagar, 1997

Reference Books:
1. MACKIE &Mc CARTNEY Practical Medical Microbiology
2. SYDNEY M. FINEGOLD & ELLEN JO BARON :Diagnostic Microbiology (DM)

SESSION 1 

  • Introduction to Microbiology : Types of Microorganisms
  • Physiology of Microorganisms-Nutrition ,Enzymes ,Metabolism and Energy

 

 

.               Presentation B OM & P- PHYSIOLOGY OF MICROORGANISMS

Physiology of Microorganisms –Nutrition, Enzymes, Metabolism and energy:  

https://www.youtube.com/watch?v=H3xIOvoevU4 

https://www.youtube.com/watch?v=W5soSpVWMT8 

 

 

Introduction to Microbiology : 

Types of Microorganisms: 

https://www.youtube.com/watch?v=ei6Z7orCpPk 

https://www.youtube.com/watch?v=fU0XO1X1tAE&t=17s 

https://www.slideshare.net/RuchikaMaurya4/general-and-ocular-microbiologypptx

SESSION 2 

  • Microbial Growth
  • Control of Microbial Growth - Antimicrobial Methods and Chemotherapy

 

Microbial Growth: 

https://www.youtube.com/watch?v=JIZ7lj3y4MQ&t=16s 

https://www.youtube.com/watch?v=DfA-MHgi2ws 

Presentation

 

https://www.slideshare.net/vinayawarad/growth-of-bacteria-172092302

SESSION 3 

  • Microbes versus humans - Development of infection
  • Disease Process

 

Microbes versus Humans- The development of Infection, the disease process, pathogenicity and virulence. 

https://www.youtube.com/watch?v=pMH0nNITX3U 

 

Presentation

https://www.slideshare.net/jinx11/microbe-human-interaction

SESSION 4

SESSION 5 

  • General Pathology
  • Principles ,Pathophysiology of the Angiogenesis

Principles: 

https://www.youtube.com/watch?v=1vaEVcMfa1E 

 

Pathophysiology of Ocular Angiogenesis:  

https://www.youtube.com/watch?v=Ep_nCSEDeAE 

https://www.youtube.com/watch?v=95n4uIS06Qc 

 

SESSION 6 

  • Ocular Infections

Ocular Infections:Pathology of cornea and Conjunctiva : 

https://www.youtube.com/watch?v=ydKbESsC3es 

https://www.youtube.com/watch?v=Mtk8ooQPI9U 

 

SESSION 7

  • Pathology of Uvea and its characteristics

Pathology of Uvea: 

https://www.youtube.com/watch?v=BCkak69A2kE 

SESSION 8 

SESSION 9

SESSION 10 

  • Ocular Bacteriology introduction and differentiation

Ocular Bacteriology-Gram positive: 

https://www.youtube.com/watch?v=AZS2wb7pMo4 

SESSION 11

  • Gram Positive Bacteria example : Staphylococcus auras
  • Staphylococcus epidermis

Staphylococcus auras, Staphylococcus epidermis: 

https://www.youtube.com/watch?v=0D2mKmUvxPc 

SESSION 12 

  • Streptococcus
  • Propionic bacterium

Streptococcus: 

https://www.youtube.com/watch?v=HAtu6uNUZyY 

propionic bacterium: 

https://www.youtube.com/watch?v=-lfKbjDctF0 

SESSION 13 

  • Bacteria including acid fast Bacilli - with the staining procedure and identification .

Bacteria including acid fast bacilli: Mycobacterium tuberculosis 

https://www.youtube.com/watch?v=jyTekFxyVeY 

SESSION 14

  • Mycobacterium tuberculosis structure and characteristics

Bacteria including acid fast bacilli: Mycobacterium tuberculosis 

https://www.youtube.com/watch?v=jyTekFxyVeY 

SESSION 15

  • Mycobacterium leprae structure and characteristics

Myobacterium leprae: 

https://www.youtube.com/watch?v=oXxWvHCdx7E 

 

SESSION 16

  • Gram negative bacteria- Pseudomonas , Haemophilus

Ocular Bacteriology - Gram negative Bacteria 

https://www.youtube.com/watch?v=NWASSXDzHRs 

haemophiilus 

https://www.youtube.com/watch?v=ug-M1nIhfIA 

 

 

pseudomonas, 

https://www.youtube.com/watch?v=Dv6J-8Vi2t4 

SESSION 17

SESSION 18

  • Pathology of Retina in systemic diseases/disorders

Pathology of retina in systemic disease/disorders 

https://www.youtube.com/watch?v=HHefZIQzj8Y 

SESSION 19

  • Pathology of Eyelids

SESSION 20

  • Pathology of Adnexa

SESSION 21

  • Pathology of orbital space occupying lesions

Pathology of orbital space occupying lesions 

https://www.youtube.com/watch?v=HKEA4p5k66U 

SESSION 22

  • Spirochetes ( Treponema ,Leptospiraceae)

Spirochetes (Treponema, Leptospiraceae), 

https://www.youtube.com/watch?v=XG1gEZD3_L8 

SESSION 23

  • Virology : Classification of Viruses in ocular Diseases

Virology: Classification of Viruses in Ocular Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. , 

https://www.youtube.com/watch?v=Rpj0emEGShQ&list=PL5lHe_3BHEka0875fY_eS_YS-wcs89uZr 

SESSION 24

  • Virology : Rubella

Virology: Classification of Viruses in Ocular Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. , 

https://www.youtube.com/watch?v=Rpj0emEGShQ&list=PL5lHe_3BHEka0875fY_eS_YS-wcs89uZr 

SESSION 25

  • Virology : Adenovirus

Virology: Classification of Viruses in Ocular Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. , 

https://www.youtube.com/watch?v=Rpj0emEGShQ&list=PL5lHe_3BHEka0875fY_eS_YS-wcs89uZr 

SESSION 26

  • Virology : Onchogenenic viruses

Virology: Classification of Viruses in Ocular Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. , 

https://www.youtube.com/watch?v=Rpj0emEGShQ&list=PL5lHe_3BHEka0875fY_eS_YS-wcs89uZr 

SESSION 27

  • Virology : Oncogenic Viruses (HPV)

Virology: Classification of Viruses in Ocular Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. , 

https://www.youtube.com/watch?v=Rpj0emEGShQ&list=PL5lHe_3BHEka0875fY_eS_YS-wcs89uZr 

SESSION 28

  • Virology : Oncogenic Viruses (EBV,Retroviruses).

Virology: Classification of Viruses in Ocular Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. , 

https://www.youtube.com/watch?v=Rpj0emEGShQ&list=PL5lHe_3BHEka0875fY_eS_YS-wcs89uZr 

SESSION 29

  • Virology : Hiv

Virology: Classification of Viruses in Ocular Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. , 

https://www.youtube.com/watch?v=Rpj0emEGShQ&list=PL5lHe_3BHEka0875fY_eS_YS-wcs89uZr 

SESSION 30

  • Virology : Oncogenic Viruses (HBV)

Virology: Classification of Viruses in Ocular Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. , 

https://www.youtube.com/watch?v=Rpj0emEGShQ&list=PL5lHe_3BHEka0875fY_eS_YS-wcs89uZr 

SESSION 31

  • Virology : Oncogenic Viruses (EBV ,RETROVIRUS )

Virology: Classification of Viruses in Ocular Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. , 

https://www.youtube.com/watch?v=Rpj0emEGShQ&list=PL5lHe_3BHEka0875fY_eS_YS-wcs89uZr 

SESSION 32

  • Intra cellular parasites
  • Chlamydia ,Protozoa

Protozoa (Taxoplasmosis, Acanthamoeba,) Helminths ( Toxocariasis , Filariasis , Onchocerciasis , Trematodes ) 

https://www.youtube.com/watch?v=B2lT_00KAgs 

SESSION 33

  • Helminths classification

Protozoa (Taxoplasmosis, Acanthamoeba,) Helminths ( Toxocariasis , Filariasis , Onchocerciasis , Trematodes ) 

https://www.youtube.com/watch?v=B2lT_00KAgs 

SESSION 34

SESSION 36

  • Pathology of LENS

Pathology of Lens 

https://www.youtube.com/watch?v=d5D0B2PoC7U 

 

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