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• To Know the basic of Bio-Organic chemistry and its application in industry
• This course gives idea about the structure of different bases of nucleic acid, DNA and RNA.
• Number of amino acids, there functions and the peptide bond that connect di, tri and polypeptides.
• To study about proteins, lipid and carbohydrates
• After completion of the course, students will able to understand regarding the essential and nonessential amino acids and can predict how their ionic charges change with pH.
• They know how enzyme activity is regulated and affected by temperature, pH and concentration in biochemical reaction.
• It also learns about DNA replication, transcription and translation including the role of RNA.
• This course will provide a real value addition for higher study especially for research where one can apply this fundamental chemistry in making new drug molecule against various diseases
• This course gives total idea about the organic chemistry and basic biology and how it will be applicable in Industry
• This course will help students for getting jobs in pharmaceutical industry
and pursue their dream in higher study
Module I (8 h)
Nucleic Acids: Introduction of Nucleic Acids, Components of nucleic acids, Nucleosides and nucleotides, Structure, synthesis and reactions of: Adenine, Guanine, Cytosine, Uracil and Thymine, Structure of polynucleotides.
Practice 1: Isolation and characterization of DNA from cauliflower
Practice 2: Isolation and characterization of DNA from onion
Assignment 1: Structure of polynucleotides
Module II (13 h)
Amino acids, peptide and proteins: Introduction to amino acid, peptide and protein, Classification of amino acid and peptides, α-Amino Acids: Synthesis, ionic properties and reactions, Zwitterions, pKa values, isoelectric point and electrophoresis, Study of peptides: determination of their primary structures-end group analysis, methods of peptide synthesis (Synthesis of peptides using N-protecting, C-protecting and C-activating groups -Solid-phase synthesis)
Practice 3: Estimation of glycine by Sorenson’s formalin method
Practice 4: Study of the titration curve of Amino acid
Practice 5: Estimation of proteins by Lowry’s method.
Assignment 2: Reactions, Zwitterions, pKa values, isoelectric point and electrophoresis
Assignment 3: α-Amino Acids: Study of peptides: determination of their primary structures-end group analysis
Module III (6 h)
Enzymes: Introduction, classification and characteristics of enzymes, Salient features of active site of enzymes, Mechanism of enzyme action, Factors affecting enzyme action, Coenzymes and Cofactors and their role in biological reactions, specificity of enzyme action (including stereospecificity), Enzyme inhibitors: Importance of enzyme inhibition phenomenon of inhibition (competitive, uncompetitive and non-competitive inhibition including allosteric inhibition).
Assignment 4: Enzyme inhibitors: Importance of enzyme inhibition phenomenon of inhibition (non-competitive inhibition including allosteric inhibition)
Module IV (10 h)
Lipids: Introduction to oils and fats (Properties and functions), Classes of Lipids (common fatty acids present in oils and fats, Examples of diff. Lipids), Hydrogenation of fats and oils, Saponification value, acid value, iodine number, Reversion and rancidity
Practice 6: Saponification value of an oil or a fat
practice 7: Determination of Iodine number of an oil/ fat.
Module V (8 h)
Concept of Energy in Biosystems: Introduction to metabolism (catabolism, anabolism), ATP: ATP hydrolysis and free energy change, Biological redox systems: NAD+, FAD , Conversion of food to energy, Outline of catabolic pathways of carbohydrate- glycolysis, Fermentation and Krebs cycle, Catabolic pathways of fat and protein, Metabolic pathways of protein, fat and carbohydrate
Practice 8: Extraction of starch from potatoes
Assignment 5: Outline of catabolic pathways of carbohydrate-glycolysis , Fermentation and Krebs cycle
Assignment 6: Catabolic pathways of fat and protein, Metabolic pathways of protein, fat and carbohydrate
Module VI (9 h)
Pharmaceutical Compounds: Structure and Importance: Classification, structure and therapeutic uses of antipyretics: Paracetamol (with synthesis) , Analgesics: Ibuprofen (with synthesis), Antimalarials: Chloroquine, Antibiotics and detailed study of chloramphenicol, Medicinal values of curcumin (haldi), azadirachtin (neem), vitamin C and antacid (ranitidine)
Practice 9: Preparation of triphenylmethanol via Grignard
Practice 10: Handling Pyrophoric Materials like n-Butyl Lithium
Assignment 7: Medicinal values of curcumin (haldi), azadirachtin (neem), vitamin C and antacid (ranitidine)
Module VII (12 h)
Bio-imaging and Bio-Medical science: Introduction to basic cell biology, Optical fluorescent probe and photophysical property, Optical probe for bio-imaging application, Protein labelling, Diagnostic kit and Device fabrication
Practice 11: Live Cell Imaging using small molecular probe
Practice 12: Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
Assignment 8: Diagnostic kit development
Assignment 9: Device fabrication
Berg, J.M., Tymoczko, J. L. and Stryer, L. (2006) Biochemistry. VIth Edition. W.H. Freeman and Co.
Nelson, D. L., Cox, M. M. and Lehninger, A. L. (2009) Principles of Biochemistry. IV Edition. W.H. Freeman and Co.
Murray, R. K., Granner, D. K., Mayes, P. A. and Rodwell, V.W. (2009) Harper’s Illustrated Biochemistry. XXVIII edition. Lange Medical Books/ McGraw-Hill.
Session 28 (1h)
Practice 6 (Module IV): Saponification value of an oil or a fat
Session 32 (1h)
Concept of Energy in Biosystems: Catabolism and anabolism
Srikanta Sahu completed his Ph.D. from IIT Delhi in Bio-organic chemistry under the supervision of Prof. V. Haridas. After his PhD, he moved for postdoctoral work at National University of Singapore (NUS) in the field of Bio-medical science and chemical biology under the guidance of Prof. Young Tae Chang. Later, he joined as a core […]