Pharmaceutical microbiology (Practical)

Teacher

Rasmita Jena

Category

Core Courses

Course Attendees

Still no participant

Course Reviews

Still no reviews

Course Name : Pharmaceutical microbiology (Practical)

Code(Credit) : BPHL2103(0-2-0)

Course Objectives

Study of all categories of microorganisms especially for the production of alcohol antibiotics, vaccines, and vitamins enzymes   and understand the importance of sterilization, sterility testing of pharmaceuticals. Also learn the practical skill of isolation, identification and preservation of pure cultures along with the biochemical characterization.

CO PO Mapping

CO                                                       Statements PO MAPPING
CO-1 Understand methods of identification, isolation and preservation of various microorganisms PO1, PO6
CO-2 To understand the importance and implementation of sterilization in

pharmaceutical processing and industry

PO1, PO6, PO10, PO11
CO-3 Learn sterility testing of pharmaceutical products. PO1, PO10, PO11
CO-4 Carried out microbiological assay techniques. PO1, PO5, PO10
CO-5 To acquire and demonstrate competency in laboratory safety and in routine and specialized microbiological laboratory skills applicable to microbiological research or clinical methods, including accurately reporting observations and analysis. PO1, PO5, PO11

Course Syllabus

1. Introduction and study of different equipments and processing, e.g., B.O.D. incubator,
laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator,
microscopes used in experimental microbiology.
2. Sterilization of glassware, preparation and sterilization of media.
3. Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations.
4. Staining methods- Simple, Grams staining and acid fast staining (Demonstration with
practical).
5. Isolation of pure culture of micro-organisms by multiple streak plate technique and other
techniques.
6. Microbiological assay of antibiotics by cup plate method and other methods
7. Motility determination by Hanging drop method.
8. Sterility testing of pharmaceuticals.
9. Bacteriological analysis of water
10. Biochemical test

Session Plan

Experiment- 1

1. Introduction and study of different equipments and processing, e.g., B.O.D. incubator,laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator,microscopes used in experimental microbiology.

1.Tools in Microbiology Laboratory1.Tools in Microbiology Laboratory-converted

https://www.youtube.com/watch?v=2hDuPEHNOAY&ab_channel=MicrobiologyEasyNotes

 

Experiment- 2

2. Sterilization of glassware, preparation and sterilization of media

2.Sterilization-converted

https://www.youtube.com/watch?v=LSu8YmW4mhM&ab_channel=Solution-Pharmacy

Experiment-3

3. Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations

3.CULTURAL CHARACTERISTICS OF MICROORGANISMS-converted

https://www.youtube.com/watch?v=-C8I4h_ul4o&ab_channel=RutujaDeshmukh

Experiment- 4

4. Staining methods- Simple, Grams staining and acid fast staining (Demonstration with practical)

4.BACTERIAL IDENTIFICATION-converted

https://www.youtube.com/watch?v=BY1scdexKIw&ab_channel=TheLearningMedico

Experiment-5

5. Isolation of pure culture of micro-organisms by multiple streak plate technique and other techniques.

5.PLATING TECHNIQUES

Experiment- 7

7. Motility determination by Hanging drop method

7.Hanging-converted

Session 8

Session 9

Case Studies

Case Studies

Our Main Teachers

Rasmita Jena

Assistant Professor
VIEW PROFILE