PHARMACEUTICAL MICROBIOLOGY_ PRACTICAL_BP 307 P

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Rasmita Jena

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PHARMACEUTICAL MICROBIOLOGY PRACTICAL BP 307 P

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COURSE OBJECTIVES

Sl. No. Objectives
01 Understand the fundamentals of microbiology and its relevance to the pharmaceutical field.
02 Demonstrate knowledge of microbial classification, structure, and growth.
03 Comply with regulatory standards and ethical guidelines in pharmaceutical microbiology.

COURSE OUTCOMES

On successful completion of the course, the student will be able to :

COs Description CO-PO Mapping
CO-1 Define microbiological equipment and processing techniques. PO1, PO3, PO4, PO12
CO-2 Describe sterilization, sub-culturing, and staining methods. PO1, PO2,PO3,PO4, PO12
CO-3 Identify pure culture isolation and microbiological assay methods. PO1, PO2,PO3,PO4, PO12
CO-4 Explain sterility testing and bacteriological analysis. PO1, PO2,PO3,PO4, PO12
CO-5 Distinguish between different sterilization and testing techniques. PO1, PO2,PO3,PO4, PO12

COURSE SYLLABUS

SL. NO. TOPICS
1 Introduction and study of different equipments and processing, e.g., B.O.D. incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator, microscopes used in experimental microbiology
2 Sterilization of glassware, preparation and sterilization of media
3 Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations
4 Staining methods- Simple, Grams staining and acid fast staining
5 Isolation of pure culture of micro-organisms by multiple streak plate technique and other techniques.
6 Microbiological assay of antibiotics by cup plate method and other methods
7 Motility determination by Hanging drop method
8 Sterility testing of pharmaceuticals.
9 Bacteriological analysis of water
10 Biochemical test.

Experiment no. 1

Introduction and study of different equipments and processing, e.g., B.O.D. incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator, microscopes used in experimental microbiology.

1. Equipments used in microbiology

Experiment no. 2

Sterilization of glassware, preparation and sterilization of media.

2. Sterilization of glasswares

Experiment no.3

Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations.

3. Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations.

Experiment no.4

Staining methods- Simple  staining.

4. Staining methods- Simple  staining.

Experiment no.5

Staining methods- Grams staining.

5. Staining methods- Grams staining.

Experiment no.6

Staining methods-  acid fast staining.

6. Staining methods-  acid fast staining.

Experiment no. 7

Isolation of pure culture of micro-organisms by multiple streak plate technique and other techniques.

7. Isolation of pure culture

Experiment no. 8

Isolation of pure culture of micro-organisms by pour plate technique.

8. Isolation of pure culture

Experiment no. 9

Isolation of pure culture of micro-organisms by swab/spread plate technique.

9. Isolation of pure culture

Experiment no. 10

Microbiological assay of antibiotics by cup plate method.

10. Microbiological assay of antibiotics by cup plate method.

Experiment no. 11

Microbiological assay of antibiotics by disc diffusion method.

11. Microbiological assay of antibiotics by disc diffusion method.

Experiment no.12

Motility determination by Hanging drop method.

12. Motility determination test by hanging drop method

Experiment no. 13

Sterility testing of pharmaceuticals.

13. sterility test of pharmaceuticals

Experiment no. 14

Bacteriological analysis of water.

14. Bacteriological analysis of water

Experiment no. 15

Biochemical test (IMViC).

15. Biochemical test

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Rasmita Jena

Assistant Professor
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