PHARMACEUTICAL MICROBIOLOGY PRACTICAL BP 307 P
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PHARMACEUTICAL MICROBIOLOGY PRACTICAL BP 307 P
CREDIT: 0-2-0
COURSE OBJECTIVES
Sl. No. | Objectives |
01 | Understand the fundamentals of microbiology and its relevance to the pharmaceutical field. |
02 | Demonstrate knowledge of microbial classification, structure, and growth. |
03 | Comply with regulatory standards and ethical guidelines in pharmaceutical microbiology. |
COURSE OUTCOMES
On successful completion of the course, the student will be able to :
COs | Description | CO-PO Mapping |
CO-1 | Define microbiological equipment and processing techniques. | PO1, PO3, PO4, PO12 |
CO-2 | Describe sterilization, sub-culturing, and staining methods. | PO1, PO2,PO3,PO4, PO12 |
CO-3 | Identify pure culture isolation and microbiological assay methods. | PO1, PO2,PO3,PO4, PO12 |
CO-4 | Explain sterility testing and bacteriological analysis. | PO1, PO2,PO3,PO4, PO12 |
CO-5 | Distinguish between different sterilization and testing techniques. | PO1, PO2,PO3,PO4, PO12 |
COURSE SYLLABUS
SL. NO. | TOPICS |
1 | Introduction and study of different equipments and processing, e.g., B.O.D. incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator, microscopes used in experimental microbiology |
2 | Sterilization of glassware, preparation and sterilization of media |
3 | Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations |
4 | Staining methods- Simple, Grams staining and acid fast staining |
5 | Isolation of pure culture of micro-organisms by multiple streak plate technique and other techniques. |
6 | Microbiological assay of antibiotics by cup plate method and other methods |
7 | Motility determination by Hanging drop method |
8 | Sterility testing of pharmaceuticals. |
9 | Bacteriological analysis of water |
10 | Biochemical test. |
Experiment no. 1
Introduction and study of different equipments and processing, e.g., B.O.D. incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator, microscopes used in experimental microbiology.
Experiment no. 2
Sterilization of glassware, preparation and sterilization of media.
Experiment no.3
Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations.
3. Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations.
Experiment no. 7
Isolation of pure culture of micro-organisms by multiple streak plate technique and other techniques.
Experiment no. 8
Isolation of pure culture of micro-organisms by pour plate technique.
Experiment no. 9
Isolation of pure culture of micro-organisms by swab/spread plate technique.
Experiment no. 10
Microbiological assay of antibiotics by cup plate method.
10. Microbiological assay of antibiotics by cup plate method.
Experiment no. 11
Microbiological assay of antibiotics by disc diffusion method.
11. Microbiological assay of antibiotics by disc diffusion method.
Experiment no.12
Motility determination by Hanging drop method.