PHARMACOGNOSY AND PHYTOCHEMISTRY- II (PRACTICAL)

Teacher

ABHISEK SAHU

Category

Core Courses

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COURSE NAME: PHARMACOGNOSY AND PHYTOCHEMISTRY II (PRACTICAL)

 

CODE (CREDIT): BPHL 3103 (0-2-0)

Course Objectives

 

The main purpose of subject is to impart the students the knowledge of how the secondary metabolites are produced in the crude drugs, how to isolate and identify and produce them industrially. Also this subject involves the study of producing the plants and phytochemicals through plant tissue culture, drug interactions and basic principles of traditional system of medicine.

Course Outcomes

CO Statement Mapping with POs
CO-1  To know the modern extraction techniques, characterization and identification of the herbal drugs and phytoconstituents.  PO1, PO2, PO6
CO-2 To understand the preparation and development of herbal formulation. PO1, PO6, PO8
CO-3 To understand the herbal drug interaction. PO1, PO8, PO9
CO-4 To carryout isolation and identification of phytoconstituents. PO1, PO3, PO8
CO-5 To get professional, practical skills in extraction isolation and phytochemical analysis of natural products. PO1, PO6, PO8

Course Syllabus

 

1. Morphology, histology and powder characteristics & extraction & detection of: Cinchona, Cinnamon, Senna, Clove, Ephedra, Fennel and Coriander
2. Exercise involving isolation & detection of active principles
a. Caffeine - from tea dust.
b. Diosgenin from Dioscorea
c. Atropine from Belladonna
d. Sennosides from Senna
3. Separation of sugars by Paper chromatography
4. TLC of herbal extract
5. Distillation of volatile oils and detection of phytoconstitutents by TLC
6. Analysis of crude drugs by chemical tests: (i) Asafoetida (ii) Benzoin (iii)
Colophony (iv) Aloes (v) Myrrh

Recommended Books: (Latest Editions)
1. W.C.Evans, Trease and Evans Pharmacognosy, 16th edition, W.B. Sounders & Co., London, 2009.
2. Mohammad Ali. Pharmacognosy and Phytochemistry, CBS Publishers &
Distribution, New Delhi.
3. Text book of Pharmacognosy by C.K. Kokate, Purohit, Gokhlae (2007), 37th Edition, Nirali Prakashan, New Delhi.
4. Herbal drug industry by R.D. Choudhary (1996), Ist Edn, Eastern Publisher, New Delhi.
5. Essentials of Pharmacognosy, Dr.SH.Ansari, IInd edition, Birla publications, New Delhi, 2007
6. Herbal Cosmetics by H.Pande, Asia Pacific Business press, Inc, New Delhi.
7. A.N. Kalia, Textbook of Industrial Pharmacognosy, CBS Publishers, New Delhi, 2005.
8. R Endress, Plant cell Biotechnology, Springer-Verlag, Berlin, 1994.
9. Pharmacognosy & Pharmacobiotechnology. James Bobbers, Marilyn KS, VE Tylor.
10. The formulation and preparation of cosmetic, fragrances and flavours.
11. Remington’s Pharmaceutical sciences.
12. Text Book of Biotechnology by Vyas and Dixit.

Session Plan

 

session -1

Morphology, histology and powder characteristics & extraction & detection of:
Cinchona

01.

https://www.youtube.com/watch?v=9FdeKh9am3w

session -2

Morphology, histology and powder characteristics & extraction & detection of:
Cinnamon

02

https://www.youtube.com/watch?v=oYfQq7XZNtM

session -3

Morphology, histology and powder characteristics & extraction & detection of:
Senna

3.

https://www.youtube.com/watch?v=oYfQq7XZNtM

session -4

Morphology, histology and powder characteristics & extraction & detection of:
Clove

4.

https://www.youtube.com/watch?v=96iv2yDMfB0

session -5

Morphology, histology and powder characteristics & extraction & detection of:
Ephedra

5

https://www.youtube.com/watch?v=aQAo8RapkeU

session -6

Morphology, histology and powder characteristics & extraction & detection of:
Fennel

6.

https://www.youtube.com/watch?v=HOmgf9Q539A

session -7

Morphology, histology and powder characteristics & extraction & detection of:
Coriander

https://www.youtube.com/watch?v=vwmpZF2oMTo

7.

session -8

Exercise involving isolation & detection of active principles
Caffeine - from tea dust.

8.

https://www.youtube.com/watch?v=lmf8gWbDLxc

session -9

Exercise involving isolation & detection of active principles
Diosgenin from Dioscorea

9.

https://www.youtube.com/watch?v=vWC8vJ4aZjc

session -10

Exercise involving isolation & detection of active principles
Atropine from Belladonna

10.

https://www.youtube.com/watch?v=ZoGoNDWumbM

session -11

Exercise involving isolation & detection of active principles
Sennosides from Senna

11.

https://www.youtube.com/watch?v=MtHqWgaqlFc

session -12

Separation of sugars by Paper chromatography

12.

https://www.youtube.com/watch?v=KaO4p_GqLLo

session -13

TLC of herbal extract

13.

https://www.youtube.com/watch?v=l2Xw7Tm6cD0

session -14

Distillation of volatile oils and detection of phytoconstitutents by TLC

14.

https://www.youtube.com/watch?v=W2tSMxepwcU

session -15

Analysis of crude drugs by chemical tests: (i) Asafoetida (ii) Benzoin (iii)
Colophony (iv) Aloes (v) Myrrh

15.

https://www.youtube.com/watch?v=XVKzdg-24_4

https://www.youtube.com/watch?v=hcSTVOJAKQ8

https://www.youtube.com/watch?v=Zfg-Tgi6uJA

https://www.youtube.com/watch?v=fhE7DynmBfc

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