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•Deciphering the genetic makeup and protein population in an organism are among the elementary approaches in biological sciences.
•To provide an understanding in genomics and proteomics and, the different approaches and techniques employed in these fundamental fields of study.
•Identifying various omics approaches and their role in understanding plant function.
•Design and develop experiments to understand and manipulate plant function.
•Analyse information from plant genomic research and recognise its potential applications in crop improvement.
Module I: Basics of Genomics
Introduction to genomics, Brief overview of prokaryotic and eukaryotic genome organization, Extra-chromosomal DNA: bacterial plasmids, Extra-chromosomal DNA: mitochondria and chloroplast
Practice: Demonstration of Polymerase chain reaction (PCR)
Module II: Genome mapping
Genetic and physical mapping, Markers for genetic mapping, FISH technique in gene mapping, comparative gene mapping
Practice: Demonstration of gene mapping
Module III: Genome sequencing projects
Human Genome Project, Genome sequencing projects for microbes, Genome sequencing projects for plants and animals, Accessing and retrieving genome project information from the web
Practice: Demonstration of web tools for genome analysis
Module IV: Comparative genomics
Identification and classification of organisms using 16S rRNA typing/sequencing, Identification and classification of organisms using SNPs, Use of genomes to understand evolution of eukaryotes, track emerging diseases and design new drugs, Determining gene location in genome sequence.
Practice: Phylogenetic analysis using genomic tools
Module V: Proteomics
Introduction to proteomics, Proteomics technologies- 2D-PAGE, isoelectric focusing, Mass spectrometry, MALDI-TOF, Yeast 2-hybrid system, Proteome databases.
Practice: Demonstration of 2D-PAGE gel
Module VI: Functional genomics and proteomics
Transcriptome analysis for identification and functional annotation of gene, Gene function- forward and reverse genetics, Protein chips and functional proteomics, Clinical and biomedical applications of proteomics; Introduction to metabolomics, lipidomics, metagenomics and systems biology.
Module VII: Emerging Technologies
Introduction to gene editing, ZFNs & TALENs, CRISPR/Cas System, Case studies of gene editing for crop improvement
Practice: Demonstration of gene cloning
Dr Rukmini Mishra is presently working as an Assistant Professor (Botany), School of Applied Science, Centurion University of Technology and Management. She has a PhD in Agricultural Biotechnology from ICAR-National Rice Research Institute, India. After PhD, she received the prestigious Young Scientist Award from the Dept. of Science & Technology, SERB, Govt. of India to […]