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Plant Metabolism

Plant Metabolism

Teacher

Dr. Shantanu Bhattacharyya

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Core Courses

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Course Name : Plant Metabolism

Code(Credit) : CUTM1467(3-2-1)

Course Objectives

Explain the purposes of and relationship between photosynthesis and respiration in plants.

Explain the significance of plant mass gain and loss to larger-scale ecosystem processes, such as the global carbon cycle.

Describe the sources and sinks involved in the acquisition and utilization of carbon in plant systems..

Learning Outcomes

Upon successful completion of this course, the student will be able to:

Understand on the kingdoms of biomolecules, metabolites and pathways that are the prerequisites and consequences of physiological phenomenon for further manipulations.

Develop integrative approach for visions in biological problems

Have a comprehensive understanding of the field of plant metabolism at a level that allows experimental implementation, including available methodologies and the historical development and current understanding of the field such that the interactions between various plant metabolic processes can be understood and appreciated

Course Syllabus

Module-I Concept of metabolism

Introduction,

Anabolic and Catabolic pathways,

Regulation of metabolism,

Role of regulatory enzymes (allosteric, covalent modulation and Isozymes).

Module-II Carbon assimilation

Historical background photosynthetic pigments,

Role of photosynthetic pigments (chlorophylls and accessory pigments), antenna molecules and reaction centres,

Photochemical reactions, photosynthetic electron transport, PSI, PSII, Qcycle,

CO2 reduction,

Photorespiration,

C4 pathways;

Crassulacean acid metabolism;

Factors affecting CO2 reduction.

Practice:

Chemical separation of photosynthetic pigments.

Experimental demonstration of Hill’s reaction.

To study the effect of light intensity on the rate of photosynthesis.

Effect of carbon dioxide on the rate of photosynthesis.

Demonstration of fluorescence by isolated chlorophyll pigments.

Demonstration of absorption spectrum of photosynthetic pigments

Module-III Carbohydrate metabolism

Synthesis and catabolism of sucrose and starch.

Carbon Oxidation

Glycolysis :

Fate of pyruvate,

Regulation of glycolysis,

Oxidative pentose phosphate pathway,

Oxidative Decarboxylation of Pyruvate,

Regulation of PDH, NADH shuttle;

TCA cycle,

Amphibolic role, Anaplerotic Reactions,

Regulation of the Cycle,

Mitochondrial Electron Transport,

Oxidative Phosphorylation,

Cyanide-Resistant Respiration,

Factors affecting respiration.

Practice

To compare the rate of respiration in different parts of a plant.

To compare the rate of respiration in different germinating Seed.

 

Module-IV ATP-Synthesis

Mechanism of ATP synthesis,

Substrate level phosphorylation,

Chemiosmotic mechanism(oxidative and photophosphorylation),

ATP synthase,

Boyers conformational model,

Role of uncouplers.

Module-V Lipid metabolism

Synthesis and breakdown of triglycerides,

β-oxidation,

glyoxylate cycle,

gluconeogenesis and its role in mobilisation of lipids during seed germination,

α oxidation.

Practice:

To study the activity of lipases in germinating oilseeds

Demonstration of mobilization of lipids during germination.

Module-VI Nitrogen metabolism

Nitrate assimilation,

Biological nitrogen fixation (examples of legumes and non-legumes);

Physiology and biochemistry of nitrogen fixation;

Ammonia assimilation and transamination.

Practice:

To demonstrate activity of Nitrate reductase in germinating leaves of Gram, Pea, Mung

To demonstrate activity of Nitrate reductase in germinating leaves of Rice, Wheat

Module-VII Mechanisms of signal transduction

Receptor-ligand interactions;

Second messenger concept,

Calcium calmodulin,

MAP kinase cascade

 

Text Book:

1. Hopkins, W.G. and Huner, A. (2008). Introduction to Plant Physiology.John Wiley and Sons. U.S.A. 4thedition.

2.Taiz, L., Zeiger, E., MØler, I.M. and Murphy, A (2015). Plant Physiology and Development.Sinauer Associates Inc. USA. 6thedition.

Reference Book:

 Harborne, J.B. (1973). Phytochemical Methods.John Wiley & Sons. New York

Session Plan

Session 1

Session 4

Role of regulatory enzymes (allosteric, covalent modulation and Isozymes).

https://www.youtube.com/watch?v=LKiXfqaWNHI

Session 5

Session 6 ( Assignment 2)

Historical background photosynthetic pigments,Role of photosynthetic pigments(chlorophylls and accessory pigments), antenna molecules and reaction centres,

http://www.life.illinois.edu/govindjee/photosynBook/Chapter9.pdf

https://www.youtube.com/watch?v=pwymX2LxnQs

Session 7 (Practice 1)

Chemical separation of photosynthetic pigments.

http://vlab.amrita.edu/?sub=3&brch=64&sim=160&cnt=1

Session 8 (Practice 2)

Demonstration of absorption spectrum of photosynthetic pigments

Session 10 (Practice 3)

Session 12 ( Assignment 3)

Session 15

Session 16 (Practice 6)

Demonstration of fluorescence by isolated chlorophyll pigments

Session 17 (Assignment 4)

Session 19 (Assignment 5)

Session 20

Glycolysis :Fate of pyruvate,Regulation of glycolysis,

glycolysis ppt

https://www.youtube.com/watch?v=uWOURkrxpH4

Session 23

TCA cycle, amphibolic role, anaplerotic reactions,Regulation of the cycle

Krebs (Citric Acid) Cycle PDF

https://www.youtube.com/watch?v=rr7IRYLqleg

 Session 26 (Practice 7)

To compare the rate of respiration in different parts of a plant.

https://amrita.olabs.edu.in/?sub=79&brch=17&sim=204&cnt=1

Session 32 (Practice 8)

To compare the rate of respiration in different germinating Seed.

https://www.youtube.com/watch?v=xtZhgu2EDGA

Session 33 (Assignment 9)

Session 34

 Session 36 (Practice 9)

To study the activity of lipases in germinating oilseeds and demonstrate mobilization of lipids during germination

Session 39

Role of gluconeogenesis in mobilisation of lipids during seed germination,

gluconeogenesis in germinating seed PDF

Session 40 (Practice 10)

Demonstration of mobilization of lipids during germination

Session 42 (Practice 11)

To demonstrate activity of Nitrate reductase in germinating leaves of Gram, Pea, Mung

https://www.youtube.com/watch?v=XrhRAwsjrJw&t=4s

Session 43 (Assignment 10)

Session 44

Session 45

Session 46 (Practice 12)

To demonstrate activity of Nitrate reductase in germinating leaves of Rice, Wheat

Session 49

Case Studies

Our Main Teachers

Dr. Shantanu Bhattacharyya

Assistant Professor, Department of Botany, School of Applied Science ( Bolangir Campus)
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Dr. Shantanu Bhattacharyya works as an Assistant Professor, Department of Botany, at the Centurion University of Technology and Management, Bolangir Campus, Odisha, India. He has received his M.Sc. (Life Science- Botany) and M.Phil (Life Sciences) from Sambalpur University, Orissa, India and B.Ed. from Singhania University, Rajasthan India. He qualified GATE-2007, CSIR-UGC NET LS -2008. He […]