Plant Physiology and Metabolism


Dr. Shantanu Bhattacharyya


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Course Name : Plant Physiology and Metabolism

Code(Credit) : CUTM1428(3-1-0)

Course Objectives

  • Students will learn the basic knowledge of stress adaptations in biological systems.
  • They will learn molecular understanding of primary and secondary metabolic process.

Learning Outcomes

• Acquire knowledge in various physiological processes occur in plants.
• Learn about the response of plants to various biotic and abiotic stresses.
• To enable the students to gain the ability to conduct research, pursue life long learning and provide solutions to public issues related to agriculture.

Course Syllabus

Photosynthesis - Light harvesting complexes; Red drop and Emerson's enhancement effect. Photolysis of water, photophosphorylation, mechanisms of electron transport; Hill Reaction, photoprotective mechanisms; CO2 fixation-C3, C4 and CAM pathways. Photorespiration, Chlororespiration: The relation between Photosynthesis, respiration and Chlororespiration. Regulation of C3 pathway.


Isolation of chloroplast and measurement of electron transport activity


Solute transport and photoassimilate translocation – uptake, transport and translocation of water, ions, solutes and macromolecules from soil, through cells, across membranes, through xylem and phloem; transpiration; mechanisms of loading and unloading of photoassimilates.

Comparative study of photosynthetic pigment in C3 and C4 plant, grown in light and shade condition

Plant growth regulator & Elicitors: Physiological effect & mechanism of action of Auxin, Gibberellin, Cytokinin, Ethylene, Abscisic acid, Jasmonic acid & Salicylic acid, hormone receptor, Signal transduction & Gene expression . Programmed cell death. Sensory photobiology - Structure, function and mechanisms of action of phytochromes, cryptochromes and phototropins; stomatal movement; photoperiodism and biological clocks.


To study qualitative and quantitative characters of plant community by quadrat method.

Stress Physiology: Plant responses to biotic and abiotic stress, mechanisms of biotic and abiotic stress tolerance, water deficit and drought resistance, salinity stress, metal toxicity, freezing and heat stress, oxidative stress. Metabolism: reactive oxygen species (ROS), antioxidant enzymes: catalase, peroxidases, superoxide dismutase, glutathione transferase, glutathione reductase, Halliwell–Asada cycle.


Extraction of pigments from leaves and preparation of absorption spectra for chlorophyll and carotenoids.

Cellular Respiration: Glycolysis, Fermentation, TCA cycle and their regulation. Pentose phosphate path ways, alternate oxidase. Shuttle system: Malate - Aspartate shuttle and Glycerol phosphate shuttle. Factor affecting respiration. Gluconeogenesis and its regulation.


To compare chlorophyll content in juvenile and matured leaves

Oxidative phosphorylation: Mitochondrial electron transport, Chemiosmotic mechanism, ATP-Synthesis: Mechanism of ATP synthesis, Substrate level phosphorylation, oxidative phosphorylation, ATP synthase, Boyers conformational model, Racker’s experiment, role of uncouplers. Inhibitors of oxidative phosphorylation.


Preparation of standard curve for quantification of protein, carbohydrate and reducing sugar.

Module- VII
Lipid metabolism: Fatty acid biosynthesis, synthesis of membrane lipids, storage lipids and their catabolism. α and β-oxidation of fatty acid. Glyoxalate cyle. Nitrogen fixation & Metabolism: Biological Nitrogen fixation, asymbiotic and symbiotic Nitrogen fixation, nodule formation, Nod and Nif genes their regulation and function, mechanism of nitrate uptake and reduction, ammonium transport and assimilation.


Text Books:
• Devlin, R. N. and Witham, F. H. (1983). Plant Physiology. CBS Publishers, Delhi.
• Bhattacharya D. (1999). Experiments in Plant Physiology- A Laboratory Manual. Narosa Publishing House, New Delhi.
• Pandey and Sinha (1987)., Plant Physiology, Vikas Publishing House.
• Satyanarayana, U. and Chakrapani, U.(2013), Biochemistry, Elsevier

Reference Book:
• Taiz, L., Zeiger, E., Mller, I.M. and Murphy, A (2015). Plant Physiology and Development. Sinauer Associates Inc. USA. 6th edition.

Session 12 (Assignment-2) 1 hour

Session 18 (Assignment) 1 hour

Session 21 (Assignment) 1 hour

Session 25 (Assignment) 1 hour

Session 30 (Assignment) 1 hour

Case Studies

Case Studies

Our Main Teachers

Dr. Shantanu Bhattacharyya

Assistant Professor, Department of Botany, School of Applied Science ( Bolangir Campus)

Dr. Shantanu Bhattacharyya works as an Assistant Professor, Department of Botany, at the Centurion University of Technology and Management, Bolangir Campus, Odisha, India. He has received his M.Sc. (Life Science- Botany) and M.Phil (Life Sciences) from Sambalpur University, Orissa, India and B.Ed. from Singhania University, Rajasthan India. He qualified GATE-2007, CSIR-UGC NET LS -2008. He […]

Mr. Srimay Pradhan

Assistant Professor in Department of Botany, School of Applied Sciences, Centurion University of Technology and Management

Mr. Srimay Pradhan is presently working as an assistant professor (Botany) in School of applied Sciences, Centurion University of Technology and Management. Apart from this, he is also persuing his doctoral research on diversity, distribution and ethnomedicinal uses of Lichens from Centurion University of Technology and Management. He has completed his Master (Botany) from Department […]