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Theory
1.1. Classification of Plant Breeding methods
1.2. Genetic basis and breeding methods in self- pollinated crops
1.3. Hybridization techniques and handling of segregating population
1.4. Genetic basis and breeding methods of cross-pollinated crops
1.5. Heterosis and inbreeding depression
1.6. Hybrid breeding- steps /operations in production of hybrid varieties
1.7. Male sterility and its application in hybrid seed production
1.8. Breeding methods in asexually propagated crops
1.9. Special breeding techniques: Mutation breeding
1.10. Special breeding techniques: Breeding for abiotic stresses
1.11. Special breeding techniques: Breeding for biotic stresses
1.12. DNA markers and its application in plant breeding
1.13. Marker assisted selection (MAS) and QTL
1.14. Cultivar development-variety testing procedures, release and notification system in India and IPR
Practical
1.1. Emasculation and hybridization techniques in Rice
1.2. Emasculation and hybridization techniques in maize
1.3. Emasculation and hybridization techniques in vegetable crops: Tomato
1.4. Emasculation and hybridization techniques in vegetable crops: Okra
1.5. Emasculation and hybridization techniques in vegetable crops: Brinjal
1.6. Handing of segregating populations
1.7. Designs used in plant breeding experiment: RBD analysis
1.8. Estimation of heterosis, inbreeding depression and heritability
1.9. Learning techniques in hybrid seed production using male-sterility in field crops
1.10. Use of male sterility in hybrid seed production: Rice
1.11. Genetic Mechanisms for Hybrid Seed Production in vegetable crops
1.12. Visit to AICRP plots to know about different trials for cultivar development
1.13. DNA isolation, DNA purity and quantification tests
1.14. DNA finger printing using PCR-based DNA markers
1.15. Gel electrophoresis
1.16. Gel scoring and data analysis for tagging and phylogenetic relationship
1.17. Use of standard MAS procedures- screening crops for resistant to different biotic stress traits
1.18. Use of standard MAS procedures- screening crops for resistant to different abiotic stress traits
Suggested Readings
Theory
2.1. Importance and scope of vegetable seed production in India
2.2. Planning for certified, foundation and breeder seed production
2.3. Seed quality: Certification standards for self and cross pollinated and vegetatively propagated crops
2.4. Principles of seed production in vegetable crops
2.5. Seed production in varieties and hybrids of cereals crops: rice and maize
2.6. Seed production in varieties and hybrids of vegetable crops: tomato, brinjal and okra
2.7. Harvesting/picking stage and seed extraction in vegetables
2.8. Seed drying, seed processing and seed storage
2.9. Seed health and measures for pest and disease control during storage
Practical
2.1. Selection of suitable areas/locations, seed/planting material for quality seed production
2.2. Determination of planting ratios for hybrid seed production of cereals
2.3. Study of floral biology of vegetable crop: Tomato
2.4. Study of floral biology of vegetable crop: Okra
2.5. Study of floral biology of vegetable crop: Eggplant
2.6. Determination of planting ratios for hybrid seed production of vegetables
2.7. Seed extraction methods and their effect on quality of vegetables
2.8. Seed and seedling vigour test
2.9. Practice on seed production of varieties and hybrids in cereal crops
2.10. Practice on seed production of varieties and hybrids in vegetable crops
2.11. Visit to seed production farms
2.12. Visit to seed testing laboratories
2.13. Visit to seed processing unit.
Suggested Readings
Theory
3.1. Seed policy
3.2. Seed Certification: concept, objectives and importance of seed certification
3.3. Rule and regulation: Seed acts, Seed certification agency/organization and legal status
3.4. Indian Minimum Seed Certification Standards (I.M.S.C.S.)
3.5. Phases, Planning and management of seed certification programmes
3.6. Field Inspection: principles, phases and procedures, post-harvest inspection and evaluation
3.7. Seed sampling and seed testing for quality assessment
3.8. Grant of certificate and certification fee and other service charges
3.9. Seed marketing: structure and organization and pricing policy
3.10. Role of WTO and OECD in seed marketing
Practical
3.1. General procedure of seed certification
3.2. Identification of weed and other crop seeds as per specific crops
3.3. Field inspection at different stages of a crop and observations recorded
3.4. Inspection and sampling at harvesting/threshing for seed law enforcement
3.5. Inspection and sampling at processing and after processing for seed law enforcement
3.6. Sampling methods and seed quality test
3.7. Specifications for tags and labels to be used for certification purpose
3.8. Varietal Identification through Grow Out Test
3.9 Varietal Identification through Electrophoresis
3.10. Molecular test for pre- and post-harvest quality control
3.11. Biochemical test for pre- and post-harvest quality control
3.12. Visits to regulatory seed testing laboratory, including plant quarantine lab
3.1.3 Visit to private and public sector seed companies
3.14. Visit to certification agencies, and NBPGR.
Suggested Readings
Practice
4.1. Growing different genotypes of a crop using appropriate experimental design
4.2. Practicing emasculation and hybridization techniques in various crops
4.3. Harvesting of F1 seeds
4.4. DNA isolation of F1 progenies
4.5. Check the hybrid (F1) fidelity using molecular markers
4.6. Visit related research station to learn techniques in hybrid seed production using male-sterility in field crops
4.7. Visit to hybrid seed production plots: Rice, Maize
4.8. Visit to hybrid seed production plots: vegetable crops
4.9. Visit related research station to understand MAS procedures
Practice
5.1. Selection of suitable areas/locations for high quality seed/planting material production
5.2. Learn the principles of seed production of brinjal
5.3. Learn the principles of seed production of okra
5.4. Learn the principles of seed production of tomato
5.5. Field inspection at different stages of a crop and record of observations
5.6. Practice on seed extraction: dry method
5.7. Practice on seed extraction: fermentation method
5.8. Practice on seed extraction: chemical method
5.9. Practice on seed processing of vegetable crops
Practice
6.1. Learn the seed sampling methods
6.2. Seed testing: physical purity test
6.3. Seed testing: moisture test
6.4. Seed testing: germination and vigour test
6.5. Seed testing: viability test
6.6. Varietal Identification through Grow Out Test
6.7. Varietal Identification through Electrophoresis,
6.8. Molecular test for seed quality assessment
6.9. Biochemical test for seed quality assessment
6.10. Control measures for stored grain disease and pest
Session 1.1: Classification of Plant Breeding methods
Session 1.2: Genetic basis and breeding methods in self- pollinated crops
Session 1.3: Hybridization techniques and handling of segregating population
Hybridization techniques & Handing of segregation populations
Session 1.4: Genetic basis and breeding methods of cross-pollinated crops
Session 1.5: Heterosis and inbreeding depression
Heterosis, inbreeding depression, various theories of Heterosis
Session 1.6: Hybrid breeding- steps /operations in production of hybrid varieties (Exploitation of heterosis)
Session 1.7: Male sterility and its application in hybrid seed production
Session 1.8: Breeding methods in asexually propagated crops
Session 1.9: Special breeding techniques: Mutation breeding
Session 1.10: Special breeding techniques: Breeding for abiotic stresses
Session 1.11: Special breeding techniques: Breeding for biotic stresses
Breeding for resistance to disease and insect pests
Session 1.12: DNA markers and its application in plant breeding
Session 1.13: Marker assisted selection (MAS) and QTL
An_introduction_to_markers_quantitative_trait_loci
Session 1.14: Cultivar development-variety testing procedures, release and notification system in India and IPR
Session 2.1: Importance and scope of vegetable seed production in India
Session 2.2: Planning for certified, foundation and breeder seed production
Planning for certified, foundation and breeder seed production
Session 2.3: Seed quality: Certification standards for self and cross pollinated and vegetatively propagated crops
Session 2.4: Principles of seed production in vegetable crops
Session 2.5: Seed production in varieties and hybrids of cereals crops: rice and maize
Session 2.6: Seed production in varieties and hybrids of vegetable crops: tomato, brinjal and okra
Session 2.7: Harvesting/picking stage and seed extraction in vegetables crops
Physiological and harvestable maturity for seeds
Session 2.9: Seed health and measures for pest and disease control during storage
Session 3.1: Seed policy
Session 3.2: Seed policy and Seed Certification: concept, objectives and importance of seed certification
Session 3.3: Rule and regulation: Seed acts, Seed certification agency/organization and legal status
Central and state seed committee
Session 3.4: Indian Minimum Seed Certification Standards (I.M.S.C.S.)
Session 3.5: Phases, Planning and management of seed certification programmes
Session 3.6: Field Inspection: principles, phases and procedures, post-harvest inspection and evaluation
Session 3.7: Seed sampling and seed testing for quality assessment
Session 3.8: Grant of certificate and certification fee and other service charges
Session 3.9: Seed marketing: structure and organization and pricing policy
Session 1.1: Emasculation and hybridization techniques in Rice
Session 1.2: Emasculation and hybridization techniques in maize
Session 1.3: Emasculation and hybridization techniques in vegetable crops: Tomato
Session 1.4: Emasculation and hybridization techniques in vegetable crops: Okra
Session 1.5: Emasculation and hybridization techniques in vegetable crops: Brinjal
Session 1.6: Handing of segregating populations
Session 1.7: Designs used in plant breeding experiment: RBD analysis
Session 1.8: Estimation of heterosis, inbreeding depression and heritability
Estimation of heterosis, inbreeding depression and heritability
Session 1.9: Learning techniques in hybrid seed production using male-sterility in field crops
Session 1.10: Use of male sterility in hybrid seed production: Rice
Session 1.11: Genetic Mechanisms for Hybrid Seed Production in vegetable crops
Session 1.12: Visit to AICRP plots to know about different trials for cultivar development
Session 1.13: DNA isolation, DNA purity and quantification tests
Session 1.14: DNA finger printing using PCR-based DNA markers
Session 1.15: Gel electrophoresis
Session 1.16: Gel scoring and data analysis for tagging and phylogenetic relationship
Session 1.17: Use of standard MAS procedures- screening crops for resistant to different biotic stress traits
Genomics based breeding research for improving resistance to biotic and abiotic stress in cereals
Session 1.18: Use of standard MAS procedures- screening crops for resistant to different abiotic stress traits
Genomics based breeding research for improving resistance to biotic and abiotic stress in cereals
Session 2.1: Selection of suitable areas/locations, seed/planting material for quality seed production
Session 2.2: Determination of planting ratios for hybrid seed production of cereals
Session 2.3: Study of floral biology of vegetable crop: Tomato
Session 2.4: Study of floral biology of vegetable crop: Okra
Session 2.5: Study of floral biology of vegetable crop: Eggplant
Session 2.6: Determination of planting ratios for hybrid seed production of vegetables
Session 2.7: Seed extraction methods and their effect on quality of vegetables
Session 2.8: Seed and seedling vigour test
Session 2.9: Practice on seed production of varieties and hybrids in cereal crops
Session 2.10: Practice on seed production of varieties and hybrids in vegetable crops
Session 2.11: Visit to seed production farms
Session 2.12: Visit to seed testing laboratories.
Session 3.1: General procedure of seed certification
Session 3.2: Identification of weed and other crop seeds as per specific crops
Session 3.3: Field inspection at different stages of a crop and observations recorded
Crop Inspection and Harvesting | Irish Seed Trade Association (ISTA)
Session 3.4: Inspection and sampling at harvesting/threshing for seed law enforcement
Session 3.5: Inspection and sampling at processing and after processing for seed law enforcement
Session 3.6: Sampling methods and seed quality test
Session 3.7: Specifications for tags and labels to be used for certification purpose
Session 3.8: Varietal Identification through Grow Out Test
Session 3.9: Varietal Identification through Electrophoresis
Session 3.10: Molecular test for pre- and post-harvest quality control
Session 3.11: Biochemical test for pre- and post-harvest quality control
Session 3.12: Visits to regulatory seed testing laboratory, including plant quarantine lab
Session 3.13: Visit to private and public sector seed companies
Session 3.14: Visit to certification agencies, and NBPGR.
Session 4.1: Growing different genotypes of a crop using appropriate experimental design
Session 4.2: Practicing emasculation and pollination techniques in various crops
Session 4.3: Harvesting of F1 seeds
Session 4.4: DNA isolation of F1 progenies
Session 4.5: Check the hybrid (F1) fidelity using molecular markers
Session 4.6: Visit related research station to learn techniques in hybrid seed production using male-sterility in field crops
Session 4.7: Visit to hybrid seed production plots: Rice, Maize
Session 4.8: Visit to hybrid seed production plots: vegetable crops
Session 4.9: Visit related research station to understand MAS procedures
Session 5.1: Selection of suitable areas/locations for high quality seed/planting material production
Session 5.2: Learn the principles of seed production of brinjal
Session 5.3: Learn the principles of seed production of okra
Session 5.4: Learn the principles of seed production of tomato
Session 5.5: Field inspection at different stages of a crop and record of observations
Session 5.6: Practice on seed extraction: dry method
Session 5.7: Practice on seed extraction: fermentation method
Session 5.8: Practice on seed extraction: chemical method
Session 5.9: Practice on seed processing of vegetable crops
Session 6.1: Learning of seed sampling methods
Session 6.3: Seed testing: moisture test
Session 6.4: Seed testing: germination and vigour test
Wheat Seed Germination Test in Paper (Lab)
Session 6.6: Varietal Identification through Grow Out Test
Session 6.7: Varietal Identification through Electrophoresis
Session 6.8: Molecular test for quality assessment
Session 6.9: Biochemical test for quality assessment
Session 6.10: Control measures for stored grain disease and pest
On the surface, Dr. Prabhat Kumar Singh is an accomplished Assistant Professor in Genetics and Plant Breeding, MSSSoA, CUTM, Paralakhemundi, Odisha with more than 3 years of experience in teaching and research. During the PG and PhD programme, Dr. Singh worked on Mutation breeding in tuberose and grasspea respectively. As a result of dissertation work […]