Seed Production Using Manual and Molecular Methods

Teacher

Kajal Samantara

Category

Diploma Courses

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Course Name: Seed Production Using Manual and Molecular Methods

Code(Credit): ABC

 

Course Objectives

High-throughput technologies produce massive amounts of data, much too large to analyze by hand. The objective of this course is:

  • To strengthen the student’s knowledge in the subject of the breeding approach to seed production and impart training for entrepreneurship in commercial seed production
  • This course will enhance the knowledge of seed biology, seed quality, seed production, seed storage, and seed certification
  • Characterization of released and/or improved varieties/hybrids using morphological, biochemical, and molecular markers for varietal identity and registration
  • To impart training for the entrepreneurship programme
  • To initiate basic research related to genetic purity, seed health, and seed storage

Learning Outcomes

 

At the end of this course, learners are expected to learn:

  • Core competency in the subject & comparative evidence on development of seed and hybrid seed production
  • High analytical ability in understanding the application of scientific principles and students will acquire skills & handling operations of different equipment's in seed science laboratory
  • Seed production techniques of various important crops will benefit those who would like to venture into seed production companies

SUBJECT I: BREEDING APPROACH TO SEED PRODUCTION

Module: I

Floral Biology, Seed Development and Maturation (10 hr)

Floral types, structure and biology in relation to pollination mechanisms; Sporogenesis: microsporogenesis and megasporogenesis; Gametogenesis - development of male and female gametes and their structures;  Fertilization – embryo sac structure, process, barriers to fertilization, incompatibility and male sterility, factors affecting fertilization; Embryogenesis - development of typical monocot and dicot embryos; Endosperm development, modification of food storage structures with reference to crop plants; Different types of embryos, endosperm and cotyledons; Seed coat structure and development in representative crop plants; Apomixis – identification, classification, significance and its utilization in different crops for hybrid seed production; Causes of embryo abortion, embryo rescue and synthetic seeds.

Module II

Post harvest handling of seeds (10 hr)

Threshing methods; Drying - methods of seed drying; Seed processing - seed cleaning and grading; Processing equipments -cleaner cum grader; Upgrading equipments working principles - specific gravity separator, colour sorter, indented cylinder separator, spiral separator, magnetic separator, needle separator; Seed quality enhancement techniques; Importance - seed fortification, seed priming, seed coating, seed pelleting.

Module III

Seed legislation (10 hr)

Seeds Act 1966; Seed Rules 1968; Seed Control Order, 1983; and Seed Bill 2004; Seed law enforcement; Duty and powers of seed inspector, offences and penalties;  Seeds Control Order 1983; Salient features of PPV&FRA 2001; National Seed Policy 2002; and Seed Bill 2004.

Module IV

Seed Quality Testing (10 hr)

Seed quality assessment - importance; Varietal identification through grow out test, molecular and biochemical test; Detection of genetically modified crops; Field inspection principles and methods- Inspection at harvesting, threshing and processing; Pre-and post-processing quality testing of seed; Seed testing concepts and objectives, its role in seed quality control; Seed sampling, seed moisture testing, purity analysis, germination testing, tolerance limit and seed testing equipments; Quick viability test and seed standards; Seed vigour-its significance and testing methods; Post harvest inspection - processing, bagging and tagging.

Module V

Seed Storage and marketing (10 hr)

Seed storage-principles; Factors affecting seed longevity during storage; Seed treatments and packaging materials; Measures for pest and disease control during storage and godown sanitation; Seed marketing-structure and organization; Sales promotional activities; Factors affecting seed marketing and demand; International seed movement; Role of international organizations, WTO and OECD in seed trade.

Module: VI

Emerging Trends in Seed Quality Enhancement (10 hr)

Concept and significance of seed quality enhancement; Physical, chemical and pesticidal seed treatments, history, principles and methods of seed treatment, methodology and factors affecting seed enhancement treatments; Seed priming: physiological and biochemical basis, types of priming technology, biochemical and molecular changes associated, pregermiantion, film coating and pelleting, seed tapes, seed mats, seed colouring, biopriming;  Synthetic seeds – Aim and scope for synthetic seeds, historical development, somatic embryogenesis, somaclonal variation and their control, embryo encapsulation systems, hardening of artificial seeds, cryopreservation, storage of artificial seeds, desiccation tolerance, use of botanicals in improving seed quality etc.

SUBJECT II: SEED PRODUCTION TECHNOLOGY

Module: I

Principles of Seed Production (10 hr)

Introduction: Seed as basic input in agriculture; seed development in cultivated plants; seed quality concept and importance of genetic purity in seed production; types of cultivars, their maintenance and factors responsible for deterioration; seed production in self and cross pollinated crops. Mode of pollination and reproduction in crop plants and their modification in relation to hybrid seed production. Principles of hybrid seed production, isolation distance, synchronization of flowering, rouging etc. male sterility and incompatibility system in hybrid seed production, role of pollinators and their management; Seed multiplication ratios, seed replacement rate, demand and supply; suitable areas of seed production and storage, agronomy of seed production – agro climatic requirements and their influence on quality seed production; generation system of seed multiplication; maintenance of Nucleus seed, production of Breeder, Foundation and Certified seed– criteria involved; life span of a variety and causes for its deterioration; certification standards for self and cross pollinated and vegetative propagated crops. Planning of seed production for different classes of seeds for self and cross pollinated crops, Seed quality control system and organization, seed village concept; Seed production agencies, seed industry and custom seed production in India.

Module: II

Seed Production in Field Crops (10 hr)

Basic principles in seed production and importance of quality seed. Floral structure, breeding and pollination mechanism in self-pollinated cereals and millets viz, wheat, barley, paddy, ragi etc. Floral structure, breeding and pollination mechanism in cross-pollinated cereals and millets viz maize, sorghum, bajraetc ; methods and techniques of quality seed production incross-pollinated cereals and millets. Floral structure, breeding and pollination mechanism; methods and techniques of seed production in pulses (pigeon pea, chick pea, green garm, black garm, field beans, peas etc.). Floral structure, breeding and pollination mechanism; methods and techniques of seed production in major oil seeds (groundnut, castor, sunflower, safflower, rape and mustard, linseed, sesame etc.). Floral structure, breeding and pollination mechanism; methods and techniques of seed production in commercial fibers (cotton, jute, mestaetc) and vegetatively propagated crops like sugar cane, potato etc.

Module: III

Seed Production in Vegetables (10 hr)

Introduction; modes of propagation in vegetables. Seed morphology and development in vegetable seeds. Floral biology of these plant species; classification of vegetable crops based on pollination and reproduction behavior; steps in quality seed production; identification of suitable areas/locations for seed production of these crops. Classification based on growth cycle and pollination behavior; methods of seed production; comparison between different methods e.g. seed-to-seed vs. root-to-seed method in radish; seed multiplication ratios in vegetables; pollination mechanisms; sex types, ratios and expression and modification of flowering pattern in cucurbits; nursery raising and transplanting stage. Seed production technology of vegetables viz. solanaceous, cucurbitaceous, leguminous, malvaceous, cole crops, leafy vegetables, root, tuber and bulb crops and spices; harvesting/picking stage and seed extraction in fruit vegetables; clonal propagation and multiplication in tuber crops e.g. Potato, sweet potato, colocasia, tapioca; seed-plot technique in potato tuber seed production; hybrid seed production technology of vegetable crops, TPS (true potato seed) and its production technique; hybrids in vegetables; maintenance of parental lines; use of male sterility and self incompatibility in hybrid seed production, environmental factors related to flowering/bolting in vegetable crops.

Module: IV

Seed Production in Flowers, Medicinal, Fruits and Plantation Crops (10 hr)

Introduction: modes of propagation in fruits, flower and plantation crops. Floral biology of these plant species; classification of medicinal and horticultural crops based on pollination and reproduction behavior; steps in quality seed production; identification of suitable areas/locations for seed production of these crops. Flowers and Medicinal Plants; classification based on growth cycle, reproduction and pollination behavior; nursery requirement, planning and management; technology for quality seed production in important flower species i.e. marigolds, petunias, dahlia, roses, gladiolus, tulips, chrysanthemum etc; development of hybrids and their seed production technology flower plants. Seed production technology of annual medicinal plants viz. isabgol, ashawagandha etc. Fruit and Plantation Crops: role of seed in perennial plant species; classification based on reproduction and pollination behavior; polyembryony and its significance; nursery requirement, planning and management; clonal propagation and multiplication in tropical, sub-tropical and temperate fruits and plantation crops; seed orchards; seed collection, extraction and processing.  IPR issues with special reference to floral and plantation crops.

Module: V

Seed Production in Forage, Pasture and Green Manure Crops (10 hr)

Important pasture and forage legume crops in India; seed requirement and production; classification of forage, pastures and green manure crops; pollination behavior. Factors influencing seed production; maintenance of varietal purity, generation systems of seed multiplication self pollinated crops; seed production in apomictic grasses. Methods and techniques of seed production in important grasses, pastures, legumes and green manure crops; apomictic seed. Selection of seed production areas, influence of season, seed rate and spacing, sowing methods, direct seed sowing, transplanting, pelleting, fertilizer and manure requirement, isolation distance, weed control, pollination and seed setting, seed shattering, seed maturity and stage of harvest, seed collection, economics of seed production of important fodder crops. Seed processing, seed treatment, seed storage, seed viability of these crops.

Module: VI

Hybrid Seed Production (10 hr)

Heterosis: definition, expression and estimation of hybrid vigour; utilization of heterosis in agricultural, horticultural and other crop plants for crop improvement. Pre requisites for hybrid seed production; mechanisms and management of pollination in autogamous and allogamous crops; genetic constitution of varieties, hybrids and basic principles in seed production. Techniques of hybrid seed production - emasculation and crossing: use of self-incompatibility, modification of sex; types of male sterility and exploitation in hybrid development and its use in hybrid seed production; development and maintenance of A, B and R lines. Fertility restoration; use of chemical hybridizing agents, problems of non synchrony in flowering of parental lines and methods to overcome; planting ratios and population density in relation to hybrid seed yield; salient features of hybrid seed production of various crops viz., rice, sorghum, bajra, maize, sunflower, cotton and other major vegetables.

SUBJECT III: MOLECULAR APPROACH

Module: I

DNA Technology – I (10 hr)

Restriction enzymes, Modification enzymes, Ligases and nucleases, Use of restriction enzymes, Isoschizomers, Ligation procedure and applications, Homopolymer tailing, Linkers and DNA adapters, Immunodetection, DNA Methylation

Module: I

DNA Technology – II (10 hr)

DNA hybridization and cot curve, DNA Sequencing, Principles of PCR, PCR methodology, Designing PCR primers, Types of PCR – cDNA synthesis and RT-PCR, Multiplex PCR and identification of PCR products, Applications of PCR technology, Transcriptomics, Principles of pyrosequencing

Module: III

Genetic Engineering – I (10 hr)

Manipulation of purified plasmid, Classification of vectors, Artificial vectors, Different types of cloning vectors, Cosmids, Phagemids – M13, Expression vectors, Vectors for genomic library

Module: IV

Genetic Engineering – II (10 hr)

Cloning strategies for sequencing and detection of clones, Cloning strategies for expression and detection of clones, Genomic library, cDNA library, Gene transformation methods (Direct and Indirect), Introduction to transgenic plants, Application of Transgenics – Case studies

Module: V

Molecular Breeding – I (10 hr)

Types of markers, Molecular markers, Classification of molecular markers based on PCR, Classification of molecular markers based on dominance, Characterisitcs and application of molecular markers, Molecular marker with advantages and limitations – RFLP, Molecular marker with RAPD, Molecular marker with SSR, Molecular marke with AFLP, Molecular marker with AFLP.

Module: VI

Molecular Breeding – II (10 hr)

 Marker assisted selection (MAS), Applications in crop improvement, Practical case studies on MAS, QTL Mapping, Marker assisted back crossing (MABC), 16sRNA typing, Gel electrophoresis, Gel scoring and data analysis for phylogenetic relationship, Use of standard MAS procedures.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

SUBJECT I: BREEDING APPROACH TO SEED PRODUCTION

Session 1

Floral types and structure

Session 2

Floral types and biology in relation to pollination mechanisms

Session 3

Sporogenesis: microsporogenesis and megasporogenesis

Session 4

Gametogenesis - development of male and female gametes and their structures

Session 5

Male Sterility and Self-incompatability

Session 6

Embryogenesis - development of typical monocot and dicot embryos

Session 7

Endosperm Development

Session 8

Apomixis – identification, classification, significance and its utilization in different crops for hybrid seed production.

Session 9

Causes of embryo abortion

9 embryo rescue (Video)

Session 10

Embryo rescue and synthetic seeds.

Session 11

Threshing Methods

Session 12

Seed drying and methods of seed drying

Session 13

Air distribution system and types of seed dryers for heated air- drying

Session 14

Seed cleaning and methods of seed cleaning

Session 15

Seed processing equipment-Cleaner cum grader

Session 16

Upgrading equipment working principles- specific gravity separator, colour sorter and indented cylinder separator

Session 17

Upgrading equipment working principles- spiral separator, magnetic separator and needle separator

Session 18

Seed quality enhancement techniques-Seed treatment

Session 19

Seed quality enhancement techniques- special treatments(Hardening, fortification, pelleting and priming

Session 20

Seed treating equipments

Session 21

History of Seed Legislation

Session 22

Seeds Act 1966

Session 23

Seed Rules

Session 24

Seed Control Order

Session 25

Role of Seed Inspectors

Session 26

Seeds Order 1989

Session 27

Plant Varieties and Farmer's Rights

Session 28

Seed Policy 2002

Session 29

Seed Bill 2004

Session 30

Seed Bill 2019

Session 31

Seed Quality Assessment

Session 32

Molecular and biochemical tests and GMO

Session 33

Field Inspection principles-Inspection, Thresing ,Harvesting and Processing

Session 34

Pre and Post processing Quality Testing

Session 35

Seed Testing- Objective & its role in Seed technology

Session 36

Seed Sampling

Session 37

Seed Testing

Session 38

Viability test

Session 39

 

Post harvest inspection

Session 40

Seed Storage

Session 41

Seed Longevity and deterioration

Session 42

Seed Treatment

Session 43

Pre and Post processing quality testing

Session 44

Pest and Diseases control during  Storage

Session 45

Seed marketing and Organisation

Session 46

Sales promotion activities in seed marketing

Session 47

Factor affecting seed marketing and demand

Session 48

International seed Movements

Session 49

WTO in Seed Marketing

Session 50

Seed Processing and its Significance

Session 51

Seed Quality Enhancement

Seed Quality Enhancement

Video:

Session 52

Seed Treatment

Seed Treatment

Video:

Session 53

Seed Priming

Seed Priming

Video:

Session 54

Seed Coating

Seed Coating

Video:

Session 55

Seed Pelleting

Seed Pelleting

Video:

Session 56

Seed Tapes, Seed Mats, Seed Colouring

Seed Tapes, Seed Mats, Seed Colouring

Video:

Session 57

Somatic Embryogenesis

Somatic Embryogenesis

Video:

Session 58

Artificial/Synthetic seed

Artificial/Synthetic Seed

Video:

Session 59

Artificial/Synthetic Seed Hardening, Cryopreseravtion, Storage of Synthetic Seed

Artificial/Synthetic Seed Hardening, Cryopreservation, Storage of Synthetic Seed

Video:

Session 60

Use of Botanicals in Improving Seed Quality

Use of Botanicals in Improving Seed Quality

Video:

Session 60

Use of Botanicals in Improving Seed Quality

Use of Botanicals in Improving Seed Quality

Video:

Session 61

                              

Practice Session-I

                              

 Emasculation methods                                   

Session 62

Practice Session -II

Session 63

Practice session-I

Session 64

Practice session-II

Session 65

Practice Session-I

Session 66

Practice session II

Session 67

Practice session I

Session 68

Practice session II

Session 69

Objectives of seed quality enhancement, Seed enhancement techniques, Seed treatment, Equipments for seed treatment, Main  groups of chemical formulations used as seed treatment, Techniques of seed priming,  Seed pelleting and pelleting materials, and Seed pelleting process

Practice Session-I

Session 70

Seed Tapes, Seed Mats, Seed discs, Seed carpets, Seed colouring and Name of botanicals used in different crops

Practice Session-II

Session 71

Assignment I

Session 72

Quiz I

Session 73

Internal Test

Session 74

Practice Test I

SUBJECT II: SEED PRODUCTION TECHNOLOGY

Session 75

Introduction: Seed as basic input in agriculture; seed development in cultivated plants; seed quality concept and importance of genetic purity in seed production.

Session 76

Types of cultivars, their maintenance and factors responsible for deterioration; seed production in self and cross pollinated crops.

Session 77

Mode of pollination and reproduction in crop plants and their modification in relation to hybrid seed production, Principles of hybrid seed production, isolation distance, synchronization of flowering, rouging etc.

Session 78

Male sterility and incompatibility system in hybrid seed production, role of pollinators and their management.

Session 79

Seed multiplication ratios, seed replacement rate, demand and supply; suitable areas of seed production and storage.

Session 80

Agronomy of seed production – agro climatic requirements and their influence on quality seed production

Session 81

Generation system of seed multiplication; maintenance of Nucleus seed, production of Breeder, Foundation and Certified seed– criteria involved

Session 82

Life span of a variety and causes for its deterioration; certification standards for self and cross pollinated and vegetative propagated crops

Session 83

Planning of seed production for different classes of seeds for self and cross pollinated crops

Session 84

Seed quality control system and organization, seed village concept; Seed production agencies, seed industry and custom seed production in India.

session- 85

Basic principles in seed production. Seed quality and its importance in seed production

session- 86

Floral structure, breeding and pollination mechanism in self-pollinated cereals

session- 87

Floral structure, breeding and pollination mechanism in self-pollinated cereals

Session-88

Floral structure, breeding and pollination mechanism in cross-pollinated cereals

Session-89

Floral structure, breeding and pollination mechanism in cross-pollinated cereals

Session-90

Floral structure, breeding and pollination mechanism; methods and techniques of seed production in pulses

session-91

Floral structure, breeding and pollination mechanism; methods and techniques of seed production in pulses

Session-92

Floral structure, breeding and pollination mechanism; methods and techniques of seed production in major oil seeds

session-93

Floral structure, breeding and pollination mechanism; methods and techniques of seed production in major oil seeds

session-94

Floral structure, breeding and pollination mechanism; methods and techniques of seed production in commercial fibers

Session 95

Floral Biology & seed development and morphology and Modes of propagation in vegetables

Floral Biology & seed development and morphology and Modes of propagation in vegetables

Video

Session 96

Classification of vegetable crops

Session 97

Steps in quality seed production

Session 98

Hybrid seed production technology of vegetable crops

session- 99

Clonal propagation and Seed plot technique

session- 100

Seed production technology in Brinjal

session- 101

Seed Production in Bhendi & Onion

session- 102

Seed production technology of Chilli

session- 103

Seed production technology of cucurbitaceous

session- 104

Environmental factors related to flowering/bolting in vegetable crops

session- 105

Introduction: modes of propagation in fruits, flower and plantation crops

session-106

classification of medicinal and horticultural crops based on pollination and reproduction behaviour

session-107

Flowers: classification based on growth cycle, reproduction and pollination behaviour; nursery requirement, planning and management. Technology for quality seed production in important flower species.

session-108

Flowers: classification based on growth cycle, reproduction and pollination behaviour; nursery requirement, planning and management. Technology for quality seed production in important flower species.

session-109

Seed production technology of annual medicinal plants

session-110

Fruit Crops: role of seed in perennial plant species; classification based on reproduction and pollination behaviour

session-111

Fruit  Crops: role of seed in perennial plant species; classification based on reproduction and pollination behaviour

session-112

Plantation Crops: role of seed in perennial plant species; classification based on reproduction and pollination behaviour

session-113

Polyembryony and its significance; nursery requirement, planning and management.

session-114

Clonal propagation and multiplication in tropical, sub-tropical and temperate fruits and plantation crops

Session 115

Important pasture and forage legume crops in India, seed requirement and production

Important pasture and forage legume crops in India, seed requirement and production

video:

Session 116

Classification of forage, pastures and green manure crops; pollination behavior

Classification of forage, pastures and green manure crops; pollination behavior

Video:

Session 117

Factors influencing seed production; maintenance of varietal purity, generation systems of seed multiplication

Factors influencing seed production; maintenance of varietal purity, generation systems of seed multiplication

Video:

Session 118

Seed Production in Apomictic Grasses

Seed Production in Apomictic Grasses

Video:

Session 119

Berseem/Egyptian clover Seed Production

Berseem/Egyptian clover Seed Production

Video:

Session 120

Alfalfa/Lucerne Seed Production

Alfalfa/Lucerne Seed Production

Video:

Session 121

Senji (Indian Clover) Seed Production

Senji (Indian Clover) Seed Production

Video:

Session 122

Seed Processing

Seed Processing

Video:

Session 123

Seed Treatment

Seed Treatment

Video:

Session 124

Seed Storage and Seed Viability

Seed Storage and Seed Viability

Video:

Session 125

Heterosis: definition, expression and estimation of hybrid vigour

Session 126

Utilization of heterosis in agricultural, horticultural and other crop plants for crop improvement

Session 127

Pre requisites for hybrid seed production; mechanisms and management of pollination in autogamous and allogamous crops

Session 128

Techniques of hybrid seed production - emasculation and crossing, Planting ratios and population density in relation to hybrid seed yield, problems of non-synchrony in flowering of parental lines

Session 129

Self-incompatibility, its type and use in hybrid seed production

Session 130

Male sterility, its type, and exploitation in hybrid seed development, Fertility restoration; use of chemical hybridizing agents

Session 131

Development and maintenance of A, B and R lines

Session 132

Seed Production in Cereals

Session 133

Seed Production in Sunflower and Cotton

Session 134

Seed Production in Vegetables

Session 135

Seed Quality

Session 136

Floral Biology of Castor

Session 137

Floral Biology & seed development and morphology and Modes of propagation in vegetables

Practice Session-I

Session 138

Hybrid seed production technology of vegetable crops

Practice Session-II

Session 139

Practice I classification based on reproduction and pollination behaviour in Papaya

Practice I

Session 140

Practice II Introduction to methods of propagation

Practie II

Session 141

Importance of pasture and forage legume crops, Classification of forage, pastures and green manure crops, Production and Maintenance of Apomictic Hybrid, Flowers and Pollination Dynamics of Egyptian clover, and Seed Standard of Egyptian clover, Lucerne and Senji.

Practice Session-I

Session 142

Seed Processing Plant Layout Planning, Requirements in seed processing, Movement of seed in a processing plant, Seed processing equipments, Types of storage, Classification of packing materials or containers, and Maintenance of viability in storage.

Practice Session-II

Session 143

Types of Heterosis and Prediction of Performance of double ross hybrid

Practice session 1 Types of Heterosis and prediction of Performance of double cross hybrids

Session 144

Different Methods of Emasculation Techniques

Practice session 2 Different Methods of Emasculation Techniques

Session 145

Quiz II

Session 146

Quiz III

Session 147

Assignment III

Session 148

Practice Test II

Session 149

SUBJECT III- MOLECULAR APPROACH

Session 150

DNA technology-I 

Module 1

Restriction enzymes-Classification,nomenclature

Session 151

Modification enzymes

Session 152

Ligases and Nucleases

 

Session 153

Uses of restriction enzymes

Session 154

Isochizomers

Session 155

Ligation procedure and applications

Session 156

Homopolymer tailing

Session 157

Linkers and DNA adapters

Session 158

Immunodetection

Session 159

DNA methylation

Module 2

Session 160

DNA hybridization and Cot Curve

Session 161

DNA sequencing (Sanger Method)

Session 162

Polymerase Chain Reaction (PCR)-Principles of PCR

Session 163

PCR Methodology

Session 164

Designing PCR Primers

Session 165

Types of PCR, CDNA Synthesis and RT-PCR

Session 166

Multiplex PCR and Identification of PCR Products

Session 167

Applications of PCR technology

Session 168

Transcriptomics: Definition and Applications of Transcriptomics and NGS in brief

Session 169

Principles of Pyrosequencing

Session 170

Manipulation of purified plasmid

Session 171

Classification of vectors

Session 172

Artificial vectors

Session 173

Different types of cloning vectors, Plasmid-pUC 19, λ Phage.

Session 174

Cosmids

Session 175

Phagemids

Session 176

Types of expression vectors

Session 177

Vectors for genomic library in genetic engineering-Bacterial Artificial Chromosomes

Session 178

Vectors for genomic library in genetic engineering-Yeast Artificial Chromosomes

Session 179

Vectors for genomic library in genetic engineering-P1-derived Artificial Chromosomes

SUBJECT III: MOLECULAR APPROACH

Session 180

Cloning strategies for Sequencing and Detection of clones

Session 181

DNA delivery systems

Session 182

Genomic Library- strategies, construction and application

Session 183

cDNA Library-- strategies, construction and application

Session 184

Gene transformation methods

Session 185

Gene transformation methods (Indirect)

Session 186

Introduction to transgenic plants

Session 187

Application of Transgenics- case study-I

Session 188

Application of Transgenics- case study-II

Session 189

Application of Transgenics- case study-III

Session 190

Molecular Markers-Introduction

Session 191

Types of Molecular markers: Hybridization based

Session 192

Types of Molecular Markers

Session 193

Types of Molecular Markers and it's applications

Session 194

Sequence based molecular markers: SNPs

Session 195

Molecular Marker with advantages and limitations - RFLPs

Session 196

Molecular Marker with advantages and limitations -RAPD

Session 197

Molecular Marker with advantages and limitations -SSR

Session 198

Molecular Marker with advantages and limitations –AFLP

Session 199

Molecular Markers Case Study:

Session 200

Marker assisted selection (MAS) and MABC

Session 201

Marker assisted backcross breeding (MABC): Applications in crop improvement-case studies

Session 202

QTL mapping: Definition and case studies

QTL-case study

QTL case study

 

 

 

 

 

 

 

 

 

 

 

Session 203

Genome-wide Association Mapping-GWAS

Session 204

Case studies-GWAS

Session 205

Use of standard MAS procedures- screening crops for resistance to different biotic stress traits.

MAS-biotic stress resistance in crops

MAS in biotic stress resistance video

Session 206

Use of standard MAS procedures- screening crops for resistance to different abiotic stress traits.

MAS-abiotic resistance in crops

MAS in abiotic stress resistance-Video

Session 207

Mutation Breeding

Mutation breeding

Mutation Breeding vdo

Session 208

Advanced breeding tools for seed production

Session 209

RAD-Seq

Session 210

Isolation of Plant genome DNA from F1 progenies and its visualization on Agarose GEl Electrophoresis

Session 211

Isolation of RNA and its quantification

Session 212

Restriction Digestion

Session 213

Quantative determination of proteins by UV, visible Spectrophotometer

Session 214

Characterization of Proteins using SDS-Polyacrylamide gel electrophoresis (Silver staining of proteins)

Session 215

Blotting Techniques for Protein - Western blotting

Session 216

Gene amplification by Polymerase Chain Reaction (PCR) and qRTPCR

https://www.promega.in/resources/guides/nucleic-acid-analysis/pcr-amplification/

Session 217

Practice 28: Check the hybrid (F1) fidelity using molecular markers

Session 218

Molecular Tests for seed quality assessment.

seed quality acessment through molecular methods

Seed Quality assessment_vdo

Session 219

Practice 30: Biochemical test for seed quality assessment

Session 220

Assignment IV

Session 221

Quiz IV

Session 222

Final Exam

Session 223

Practice Test III

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