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COs | Course outcomes | Mapping COs with POs |
CO1 | At the conclusion of this domain, students will have expanded their experiential learning, practical knowledge, and hands-on training in the following areas: hybrid seed development using the emasculation and pollination technique; application of molecular markers in seed production; physical and genetic purity testing for high-quality seed production; and the certification process for seeds. Variety and hybrid seed production for vegetable and cereal crops; physical and genetic purity testing for high-quality seed production; several techniques for extracting seeds; and the certification process for seeds. |
PO1 |
CO2 | Launch a seed production initiative to meet the market's demand for high-quality seed and boost revenue. | PO2, PO3 |
CO3 | Marketing and distribution hybrid seed in the market so that crops can be raised sustainably. | PO4 |
PO1: Knowledge Enhancement
PO2: Professional Enhancement
PO3: Entrepreneurship Skills
PO4: Communication & Extension Skill
Theory
Classification of Plant Breeding methods,Genetic basis and breeding methods in self- pollinated crops, Hybridization techniques and handling of segregating population, Genetic basis and breeding methods of cross-pollinated crops,Heterosis and inbreeding depression,Hybrid breeding- steps /operations in production of hybrid varieties,Male sterility and its application in hybrid seed production, Breeding methods in asexually propagated crops, Special breeding techniques: Mutation breeding,Special breeding techniques: Breeding for abiotic stresses,Special breeding techniques: Breeding for biotic stresses,DNA markers and its application in plant breeding,Marker assisted selection (MAS) ,Quantitative trait analysis(QTL),Cultivar development-variety testing procedures, release and notification system in India ,IPR
Practical
Emasculation and hybridization techniques in Rice,Emasculation and hybridization techniques in maize, Emasculation and hybridization techniques in vegetable crops: Tomato,Emasculation and hybridization techniques in vegetable crops: Okra,Emasculation and hybridization techniques in vegetable crops: Brinjal,Handing of segregating populations,Designs used in plant breeding experiment: RBD analysis,Estimation of heterosis ,Inbreeding depression ,Heritability,Learning techniques in hybrid seed production using male-sterility in field crops, Use of male sterility in hybrid seed production: Rice, Genetic Mechanisms for Hybrid Seed Production in vegetable crops,Visit to AICRP plots to know about different trials for cultivar development,DNA isolation, DNA purity ,DNA quantification tests, DNA finger printing using PCR-based DNA markers,Gel electrophoresis, Gel scoring ,Data analysis for tagging,Phylogenetic relationship,Use of standard MAS procedures- screening crops for resistant to different biotic stress traits,Use of standard MAS procedures- screening crops for resistant to different abiotic stress traits, Breeding for quality traits,Breeding for drought tolerant using MAS,Breeding for salinity tolerant using MAS,Breeding for heat tolerant using MAS,Breeding for cold tolerant using MAS,Breeding for mineral toxicity using MAS,Release of new variety,Variety development through mutation breeding
Suggested Readings
2. Seed production of vegetable and cereals crops (1-2-0)
Theory
Importance and scope of vegetable seed production in India; Planning for certified, foundation and breeder seed production; Seed quality: Certification standards for self and cross-pollinated and vegetatively propagated crops; Principles of seed production in vegetable crops; Seed production in varieties and hybrids of cereals crops: paddy and maize; Seed production in varieties and hybrids of vegetable crops: tomato, brinjal and okra; Harvesting/picking stage and seed extraction in vegetables; Seed drying, seed processing and seed storage; Seed health and measures for pest and disease control during storage; Seed Invigouration; Seed Village
Practical
Selection of suitable areas/locations, seed/planting material for quality seed production; Determination of planting ratios for hybrid seed production of cereals; Study of floral biology of Paddy; Study of floral biology of Maize; Study of floral biology of Tomato; Study of floral biology of Okra; Study of floral biology of Eggplant; Determination of planting ratios for hybrid seed production of vegetables; Seed extraction methods and their effect on quality of vegetables; Seed and seedling vigour test; Practice on seed production of varieties and hybrids in cereal crops; Practice on seed production of varieties and hybrids in vegetable crops; Visit to seed production farms; Visit to seed testing laboratories; Visit to seed processing unit.
Reference
3. Seed certification (1-2-0)
Theory
Seed policy; Seed Certification; Seed Act; Seed Control Order; Indian Minimum Seed Certification Standards (I.M.S.C.S.); Phases, Planning and management of seed certification programmes; Field Inspection: principles, phases and procedures, post-harvest inspection and evaluation; Seed sampling; Seed testing; Seed Viability; Varietal Identification; Grant of certificate and certification fee and other service charges; Seed marketing: structure and organization and pricing policy; Role of WTO and OECD in seed marketing; IPR and PPV&FRA
Practical
General procedure of seed certification; Identification of weed and other crop seeds as per specific crops; Field inspection at different stages of a crop and observations recorded; Inspection and sampling at harvesting/threshing for seed law enforcement; Inspection and sampling at processing and after processing for seed law enforcement; Sampling methods and seed quality test; Seed viability testing; Seed germination; Specifications for tags and labels to be used for certification purpose; Varietal Identification through Grow Out Test; Varietal Identification through Electrophoresis; Molecular test for pre- and post-harvest quality control; Biochemical test for pre- and post-harvest quality control; Visits to Seed Testing Laboratory; Visit to private and public sector seed companies; Visit to certification agency.
Reference
Practice
Growing different genotypes of a crop using appropriate experimental design; Practicing emasculation and hybridization techniques in various crops; Harvesting of F1 seeds; DNA isolation of F1 progenies; Check the hybrid (F1) fidelity using molecular markers; Visit to research station to learn techniques in hybrid seed production using male sterility in field crops; Visit to hybrid seed production plots; Visit to research station to understand MAS procedures
Practice
Selection of suitable areas/locations for high quality seed/planting material production; Learn the principles of seed production of brinjal; Learn the principles of seed production of okra; Learn the principles of seed production of tomato; Field inspection at different stages of a crop and record of observations; Practice on seed extraction: dry method; Practice on seed extraction: fermentation method; Practice on seed extraction: chemical method; Practice on seed processing of vegetable crops
Practice
Learn the seed sampling methods; Physical Purity test; Moisture test; Germination test; Vigour test; Viability test; Varietal Identification through Grow out Test; Varietal Identification through Electrophoresis; Molecular test for seed quality assessment; Biochemical test for seed quality assessment; Control measures for stored grain disease and pest
Session 1.1: Classification of Plant Breeding methods
Session 1.2: Genetic basis and breeding methods in self- pollinated crops
Session 1.3: Hybridization techniques and handling of segregating population
Hybridization techniques & Handing of segregation populations
Session 1.4: Genetic basis and breeding methods of cross-pollinated crops
Session 1.5: Heterosis and inbreeding depression
Heterosis, inbreeding depression, various theories of Heterosis
Session 1.6: Hybrid breeding- steps /operations in production of hybrid varieties (Exploitation of heterosis)
Session 1.7: Male sterility and its application in hybrid seed production
Session 1.8: Breeding methods in asexually propagated crops
Session 1.9: Special breeding techniques: Mutation breeding
Session 1.10: Special breeding techniques: Breeding for abiotic stresses
Session 1.11: Special breeding techniques: Breeding for biotic stresses
Breeding for resistance to disease and insect pests
Session 1.12: DNA markers and its application in plant breeding
Session 1.13: Marker assisted selection (MAS)
Session 1.14: QTLs
Session 1.15: Release and notification system in India
Session 1.16: Cultivar development-variety testing procedures, release and notification system in India and IPR
Session 2.2: Planning for certified, foundation and breeder seed production
Session 2.4: Principles of seed production in vegetable crops
Session 2.5: Seed production in varieties and hybrids of Paddy
Session 2.6: Seed production in varieties and hybrids of Maize
Session 2.10: Harvesting/picking stage and seed extraction in vegetables crops
Session 2.11 - Session 2.13: Seed drying, Seed processing, Seed storage
Session 2.14: Seed health and measures for pest and disease control during storage
Session 2.15: Seed Invigouration
Session 2.16: Seed Village
Session 3.6: Phases, Planning and management of seed certification programmes
Session 3.7: Field Inspection: principles, phases and procedures, post-harvest inspection and evaluation
Session 3.8: Seed sampling
Session 3.10: Seed Viability
Session 3.11: Varietal Identification
Session 3.12: Grant of certificate and certification fee and other service charges
Session 3.13: Seed marketing: structure and organization and pricing policy
Session 3.14: Role of WTO and OECD in seed marketing
Session 3.15: IPR
Session 3.16: PPV&FRA
Session 1.1: Emasculation and hybridization techniques in Rice
Session 1.2: Emasculation and hybridization techniques in maize
Session 1.3: Emasculation and hybridization techniques in vegetable crops: Tomato
Session 1.4: Emasculation and hybridization techniques in vegetable crops: Okra
Session 1.5: Emasculation and hybridization techniques in vegetable crops: Brinjal
Session 1.6: Handing of segregating populations
Session 1.7: Designs used in plant breeding experiment: RBD analysis
Session 1.8: Estimation of heterosis
Session 1.9: Inbreeding depression
Session 1.10: Heritability
Session 1.11: Learning techniques in hybrid seed production using male-sterility in field crops
Session 1.12: Use of male sterility in hybrid seed production: Rice
Session 1.13: Genetic Mechanisms for Hybrid Seed Production in vegetable crops
Session 1.14: Visit to AICRP plots to know about different trials for cultivar development
Session 1.15: DNA isolation
Session 1.16: DNA purity
Session 1.17: DNA quantification tests
Session 1.18: DNA finger printing using PCR-based DNA markers
Session 1.19: Gel electrophoresis
Session 1.20: Gel scoring
Session 1.21: Data analysis for tagging
Session 1.22: Phylogenetic relationship
Session 1.23: Use of standard MAS procedures- screening crops for resistant to different biotic stress traits
Genomics based breeding research for improving resistance to biotic and abiotic stress in cereals
Session 1.24: Use of standard MAS procedures- screening crops for resistant to different abiotic stress traits
Genomics based breeding research for improving resistance to biotic and abiotic stress in cereals
Session 1.25: Breeding for quality traits
Session 1.26: Breeding for drought tolerant using MAS
Session 1.27: Breeding for salinity tolerant using MAS
Session 1.28: Breeding for heat tolerant using MAS
Session 1.29: Breeding for cold tolerant using MAS
Session 1.30: Breeding for mineral toxicity using MAS
Session 1.31: Release of new variety
Session 1.32: Software analysis for interpretation of molecular data
Session 2.1 - Session 2.2: Selection of suitable areas/locations, seed/planting material for quality seed production
Session 2.3 - Session 2.4: Determination of planting ratios for hybrid seed production of cereals
Session 2.5- Session 2.6: Study of floral biology of Paddy and Maize
Session 2.7 - Session 2.9: Study of floral biology of Tomato, Brinjal and Okra
Session 2.10 - Session 2.11: Determination of planting ratios for hybrid seed production of vegetables
Session 2.12 - Session 2.14: Seed extraction methods and their effect on quality of vegetables
Session 2.18 - Session 2.22: Practice on seed production of varieties and hybrids in cereal crops
Session 2.23 - Session 2.27: Practice on seed production of varieties and hybrids in vegetable crops
Session 2.28 - Session 2.30: Visit to Seed Production Farms
Session 2.31: Visit to Seed Testing Laboratory.
Session 2.32: Visit to Seed Processing Unit.
Session 3.1 - Session 3.2: General procedure of seed certification
Session 3.3 - Session 3.4: Identification of weed and other crop seeds as per specific crops
Session 3.5 - Session 3.6: Field inspection at different stages of a crop and observations recorded
Session 3.7 - Session 3.8: Inspection and sampling at harvesting/threshing for seed law enforcement
Session 3.9 - Session 3.10: Inspection and sampling at processing and after processing for seed law enforcement
Session 3.11 - Session 3.13: Sampling methods and seed quality test
Session 3.14 - Session 3.15: Seed viability testing
Session 3.16 - Session 3.17: Seed germination
Session 3.18 - Session 3.19: Specifications for tags and labels to be used for certification purpose
Session 3.20 - Session 3.24: Varietal Identification through Grow Out Test and Electrophoresis
Session 3.25 - Session 3.28: Molecular and biochemical test for pre- and post-harvest quality control
Session 3.29: Visits to Seed Testing Laboratory
Session 3.30 - Session 3.31: Visit to private and public sector seed companies
Session 3.32: Visit to certification agency
Session 4.1 - Session 4.10: Growing different genotypes of a crop using appropriate experimental design
Session 4.11 - Session 4.20: Practicing emasculation and pollination techniques in various crops.
Session 4.21 - Session 4.26: Harvesting of F1 seeds
Session 4.36 - Session 4.40: Check the hybrid (F1) fidelity using molecular markers
Session 4.41 - Session 4.42: Visit to research station to learn techniques in hybrid seed production using male-sterility in field crops
Session 4.43 - Session 4.46: Visit to hybrid seed production plots
Session 4.47 - Session 4.48: Visit to research station to understand MAS procedures
Session 5.1 - Session 5.8: Selection of suitable areas/locations for high quality seed/planting material production
Session 5.17 - Session 5.25: Learn the principles of seed production of okra
Session 5.26 - Session 5.30: Learn the principles of seed production of tomato
Session 5.31 - Session 5.35: Field inspection at different stages of a crop and record of observations
Session 5.40 - Session 5.42: Practice on seed extraction: fermentation method
Session 5.43 - Session 5.45: Practice on seed extraction: chemical method
Session 5.46 - Session 5.48: Practice on seed processing of vegetable crops
Session 6.15 - Session 6.19: Seed testing: germination and vigour test
Session 6.26 - Session 6.30: Varietal Identification through Grow Out Test
Session 6.36 - Session 6.43: Molecular and Biochemical test for quality assessment
Session 6.44 - Session 6.48: Control measures for stored grain disease and pest
Breeding Methods - Conventional and Molecular Approach
I have completed my Ph.D. in Seed Science and Technology from Bidhan Chandra Krishi Viswavidyalaya, Mohanpur. I received University Research Fellowship during the tenure of my Doctoral research. My doctoral topic was “Pre-sowing Seed Priming with Ag-Nanoparticle in Green gram: Chemo-morphic Changes during Seed Development and Response Pattern for Seed Production”. I have completed my […]
I am a Plant breeder, Now working on application of nano particles in post harvest management of horticultural crops, mainly Onion. I along with co-workers reported first time worldwide a colorimetric sensor for visual detection of post harvest spoilage of onions. I have keen interest in horticultural crops and its value addition.