Analytical Techniques
Teacher
Dr. Soumya Jal
Category
Core Courses
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Course Name : Analytical Techniques
Code(Credit) : CUTM1709 (3-1-0)
Course Objectives
- To learn the principle, instrumentation & application of Microscopy
- Principle, instrumentation & application of Centrifugation
- Chromatographic techniques
- Electrophoretic techniques
- Principle of Spectroscopy
Learning Outcomes
- After completion of the course the student will be efficient in handling the microscopy equipment’s.
- They will also be able to have idea about handling instruments like centrifuge, spectrophotometer, chromatography, flow cytometer, Automated and semi- automated Biochemistry analyzer.
- They will gain knowledge on the principle behind and the application of NMR, X-ray diffraction, ESR.
- The conceptual understanding of the subject provides opportunities for skill enhancement and scopes for higher education.
Course Syllabus
Module–1
Microscopic techniques: Principle, Instrumentation, Specimen preparation and Application: Phase–contrast microscopy, fluorescene microscopy, polarization microscopy, electron microscopy (Scanning and Transmission);
Bacterial Colony Counter (Principle and working).
Microtomy: Sectioning, Staining. Application.
Cytometry: Types, Flow cytometry and its applications.
Practice:
Demonstration of different Microscopes with their operation.
Preparing specimens for observing under microscopes.
Demonstration of Microtome.
Module-2
Centrifugation: Principle; Preparative, Analytical, Density gradient centrifugation.
Chromatography: Principles and Applications: Paper, Thin layer, Column, Ion exchange, Affinity chromatography, Gel filtration, Gas Chromatography, HPLC, FPLC.
Electrophoresis: Immuno electrophoresis, Isoelectric focusing, 2-D gel electrophoresis.
Practice:
Demonstration of Centrifuge machine
Demonstration of Chromatography techniques
Demonstration of Electrophoresis
Module-3
Colorimeter: Principle and Instrumentation; Spectrophotometry: Ultraviolet, Mass spectrometry; Flame photometry;
Principle, Instrumentation, Specimen preparation and Application of: X-ray diffraction, NMR, EPR. Principle and Application of: Fully Automated Biochemistry Analyser, Semi- automated Biochemistry Analyser, Coagulometer.
Practice:
Demonstration of Semi automated Analyzer
Demonstration of Fully automated Analyzer
Suggested Readings:
- Wilson K and Walker J. (2010). Principles and Techniques of Biochemistry and Molecular Biology. 7th Ed., Cambridge University Press.
(e-Book link: https://www.pdfdrive.com/principles-and-techniques-of-biochemistry-and-molecular-biology-e174866056.html )
- Nelson DL and Cox MM. (2008). Lehninger Principles of Biochemistry, 5th Ed., W.H. Freeman and Company.
(e-Book link: https://www.pdfdrive.com/lehninger-principles-of-biochemistry-5th-edition-d164892141.html )
- Willey MJ, Sherwood LM & Woolverton C J. (2013). Prescott, Harley and Klein’s Microbiology. 7thEd., McGraw Hill.
(e-Book link: https://www.pdfdrive.com/prescott-harley-and-kleins-microbiology-7th-ed-e188166539.html )
- Labs for Life
(e-source link: http://labsforlife.in/InstructionalVideo.aspx )
Simulation links for labs:
Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).
Online Source: Labs for life, Lecturio, Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia
Session Plan
Session 1
Topic: Microscopy
Video on Microscopy
https://assetstore.unity.com/packages/templates/packs/cky-microscope-162720
Presentation on Microscopy
Session 2
Topic: Phase–contrast microscopy
Video on Phase–contrast microscopy
https://www.youtube.com/watch?v=fCZw4X7V5Pw
Slideshare link to Phase–contrast microscopy
https://www.slideshare.net/rajpalchoudharyjat/phase-microscopy
Session 3
Topic: Fluorescence microscopy
Video on Fluorescence microscopy
https://www.youtube.com/watch?v=PCJ13LjncMc
https://www.youtube.com/watch?v=TtBja54wwXo
https://www.youtube.com/watch?v=dhAPQ_hdeP4
Slideshare link to Phase–contrast microscopy
https://www.slideshare.net/jyotsnaverma1232/fluorescence-microscopy-92806925
Session 4
Topic: Polarization microscopy
Link to Polarization microscopy
https://www.microscopyu.com/techniques/polarized-light/polarized-light-microscopy
Session 5
Topic: Electron microscopy (SEM)
Video on SEM
https://www.youtube.com/watch?v=GY9lfO-tVfE
https://www.youtube.com/watch?v=385n7o_dats
Slideshare link to Electron Microscopy
https://www.slideshare.net/gangahuvin/electron-microscopy-16995175
Session 6
Topic: Electron microscopy (SEM)
Video on TEM
https://www.youtube.com/watch?v=D7vVg77tm_4
https://www.youtube.com/watch?v=j2A6KeWrqeM
https://www.youtube.com/watch?v=_kpN2jPjRYs&pbjreload=10
Slideshare link to Electron Microscopy
https://www.slideshare.net/gangahuvin/electron-microscopy-16995175
Session 7
Practice session
Topic: Demonstration of different Microscopes with their operation and maintain technique
Video on Demonstration of different Microscopes with their operation and maintain technique
Session 8
Topic: Bacterial Colony Counter
Video on Bacterial Colony Counter
Session 9
Practice session
Topic: Preparing specimens for observing under microscopes
Video on Preparing specimens for observing under microscope
https://www.youtube.com/watch?v=aZzRygip5XY
Procedure on Preparing specimens for observing under microscope
http://www.microbehunter.com/how-can-specimens-be-prepared-for-observation/
Session 10
Topic: Microtomy: Sectioning, Staining, Application
Video on Microtome
Session 11
Practice session
Topic: Demonstration of Microtome
Video on Preparing specimens for observing under microscope
Session 12
Topic: Cytometry: Types, Flow cytometry and its applications.
Video on Cytometry: Types, Flow cytometry and its applications.
Session 13
Topic: Centrifugation Principle
Video on Centrifugation Principle
https://www.youtube.com/watch?v=IhJNFGfsUus
https://www.youtube.com/watch?v=nX0NNfV8nWw
Slideshare link to Centrifugation
https://www.slideshare.net/anurag_yadav/centrifugation-principle-and-types
Session 14
Topic: Preparative centrifuge
Video on Preparative centrifuge
Session 15
Topic: Analytical centrifuge
Video on Analytical centrifuge
Session 16
Topic: Density gradient centrifuge
Video on Density gradient centrifuge
Session 17
Practice session
Topic: Demonstration of Centrifuge machine
Video on Demonstration of Centrifuge machine
Session 18
Topic: Chromatography Principles
Video on Chromatography Principles
https://www.youtube.com/watch?v=1DxL-wfcuc0&list=PLUSfjij8XMn5VyEaLdMmWfW0DyrSpMpI_&index=10
https://www.youtube.com/watch?v=LQKNm_Q3b-k&list=PLUSfjij8XMn5VyEaLdMmWfW0DyrSpMpI_&index=9
Slideshare link to Chromatography Principles
https://www.slideshare.net/DrShabanaNazShah/chromatography-and-its-types-76207175
https://www.slideshare.net/PradeepNarwat/chromatography-152439782
Session 19
Topic: Paper Chromatography
Video on Paper Chromatography
https://www.youtube.com/watch?v=ZCzgQXGz9Tg
https://www.youtube.com/watch?v=NSJIAcfvEJI
https://www.youtube.com/watch?v=J8r8hN05xXk
Slideshare link to Chromatography Principles
https://www.slideshare.net/msaltyy/paper-chromatography-65615443
https://www.slideshare.net/karmaveerchemistry/paper-chromatography-113645350
Session 20
Practice session
Topic: Demonstration on Paper Chromatography
Video on Demonstration on Paper Chromatography
Session 21
Topic: Thin layer Chromatography
Video on Thin layer Chromatography
https://www.youtube.com/watch?v=J8r8hN05xXk
https://www.youtube.com/watch?v=tDaKxskUwA0
https://www.youtube.com/watch?v=lj5OWzhZSac
Slideshare link to Thin Layer Chromatography
https://www.slideshare.net/amulyamunni/thin-layer-chromatography-78198428
Session 22
Practice session
Topic: Demonstration on TLC
Video on Demonstration on TLC
Session 23
Topic: Column chromatography
Video on Column chromatography
https://www.youtube.com/watch?v=UmWMlKJAdSk
https://www.youtube.com/watch?v=a2wwgLV80U8
https://www.youtube.com/watch?v=lj5OWzhZSac
Slideshare link to Column Chromatography
https://www.slideshare.net/GokulKrishnan170/column-chromatography-175656931
Session 24
Practice session
Topic: Demonstration on Column Chromatography
Video on Demonstration on Column Chromatography
Session 25
Topic: Ion exchange chromatography
Video on Ion exchange chromatography
https://www.youtube.com/watch?v=wFpLgDceBhkhttps://www.youtube.com/watch?v=q3fMqgT1do8
Slideshare link to Ion exchange chromatography
https://www.slideshare.net/shiva14123/ion-chromatography-55691372
Session 26
Topic: Gel filtration chromatography
Video on Gel filtration chromatography
https://www.youtube.com/watch?v=oV5VB5kO3tQ&list=PLUSfjij8XMn5VyEaLdMmWfW0DyrSpMpI_&index=3
https://www.youtube.com/watch?v=4AMISMWWO4s&list=PLUSfjij8XMn5VyEaLdMmWfW0DyrSpMpI_&index=7
Slideshare link to Gel filtration chromatography
https://www.slideshare.net/DarkAngelWaqar/gel-filtration-chromatography
Session 27
Topic: Gas Chromatography
Video on Gas Chromatography
https://www.youtube.com/watch?v=iX25exzwKhI
https://www.youtube.com/watch?v=ZpPzImDSfqc
Slideshare link to Gas Chromatography
https://www.slideshare.net/gangadharchatterjee/gas-chromatography-38220519
Session 28
Topic: High Performance Liquid Chromatography
Video on HPLC
https://www.youtube.com/watch?v=MYSBOxbnuAw
https://www.youtube.com/watch?v=eCj0cRtJvJg
https://www.youtube.com/watch?v=kz_egMtdnL4
Slideshare link to HPLC
https://www.slideshare.net/dbasuti/hplc-high-per
https://www.slideshare.net/sagarsavale1/hplc-56392326
https://www.slideshare.net/hwrain/high-performance-liquid-chromatography-hplc-78170264
Session 29
Topic: Fast Protein Liquid Chromatography
Video on FPLC
https://www.youtube.com/watch?v=0JlHNJjfjWY
https://www.youtube.com/watch?v=TCFK5-mYvWE
https://www.youtube.com/watch?v=M_xl4aRy2rk
Presentation link to FPLC
Session 30
Topic: PAGE
Video on PAGE
https://www.youtube.com/watch?v=hCEBCIoHHzE
https://www.youtube.com/watch?v=i_6y6Z5UvwE
https://www.youtube.com/watch?v=pnBZeL8nFEo
Slideshare link to PAGE
https://www.slideshare.net/dhaRRamgol/poly-acrylamide-gel-electrophoresis-page
https://www.slideshare.net/RaviKantAgrawal/sds-page-101643445
Session 31
Practice session
Topic: Demonstration of Agarose Gel Electrophoresis
Video on Demonstration of Agarose Gel Electrophoresis
https://www.youtube.com/watch?v=6mQGNDnOyH8&t=11s
Session 32
Topic: Immuno electrophoresis
Video on Immuno electrophoresis
https://www.youtube.com/watch?v=CzfSUn0U2oQ
https://www.youtube.com/watch?v=52opF5Cc1-k
Session 33
Topic: Isoelectric focusing
Video on Isoelectric focusing
https://www.youtube.com/watch?v=YV4gR4vucwY
Slideshare link to Isoelectric focusing
https://www.slideshare.net/arushe143/isoelectric-focussing
https://www.slideshare.net/futurepaul95/isoelectric-focusing-60227596
Session 34
Topic: 2-D gel electrophoresis
Video on 2-D gel electrophoresis
https://www.youtube.com/watch?v=FHvblg338Gg
https://www.youtube.com/watch?v=JqFmnxGMc2g
https://www.youtube.com/watch?v=3wFh0z7so8w
https://www.youtube.com/watch?v=KD1pXgWLKdU
https://www.youtube.com/watch?v=Z2U0_BsVXnU&list=PLmxVzBdIHVmJaZZAAk-oEr135uHj31lrz
Slideshare link to 2-D gel electrophoresis
https://www.slideshare.net/AashishPatel14/2-d-gel-electrophoresis-73169700
https://www.slideshare.net/DarkAngelWaqar/2-d-gel-electrophoresis-96637159
Session 35
Topic: Microchip Electrophoresis
Video on Microchip Electrophoresis
https://www.youtube.com/watch?v=SoO5viJyoNA
Session 36
Topic: Colorimeter: Principle and Instrumentation
Video on Colorimeter: Principle and Instrumentation
https://www.youtube.com/watch?v=z78PBaAldRQ
https://www.youtube.com/watch?v=QdufRwbkeKo
https://www.youtube.com/watch?v=yTabfxvMdCM
Slideshare link to Coloroimeter
https://www.slideshare.net/gangadharchatterjee/colorimeter-66390783
Session 37
Topic: UV Spectrophotometry
Video on UV Spectrophotometry
https://www.youtube.com/watch?v=pxC6F7bK8CU
https://www.youtube.com/watch?v=XAp-5r3LxQo
https://www.youtube.com/watch?v=MKFjGIrRKt4
Slideshare link to UV Spectrophotometry
https://www.slideshare.net/HarpreetKaur742/uv-spectroscopy-93590000
Session 38
Topic: Mass spectrometry
Video on Mass spectrometry
https://www.youtube.com/watch?v=J-wao0O0_qM
https://www.youtube.com/watch?v=mBT73Pesiog
https://www.youtube.com/watch?v=mU_qMIGyhQ4
Slideshare link to Mass spectrometry
https://www.slideshare.net/PrincyAgarwal6/mass-spectrometry-81082759
Session 39
Topic: Flame photometry
Video on Flame photometry
https://www.youtube.com/watch?v=BBhuXOh9vOM
https://www.youtube.com/watch?v=1F6CxVF5I9g&t=23s
Session 40
Topic: X-ray diffraction
Video on X-ray diffraction
https://www.youtube.com/watch?v=QHMzFUo0NL8
https://www.youtube.com/watch?v=C1cYJthlBZY
https://www.youtube.com/watch?v=M4Wf_JT60rI
Slideshare link to X-ray diffraction
https://www.slideshare.net/gopinathkarnam/x-ray-diffraction-25472126
https://www.slideshare.net/BASANTKUMAR123/xrd-xray-diffraction
Session 41
Topic: NMR
Video on NMR
https://www.youtube.com/watch?v=ywR6aLpfjl0
https://www.youtube.com/watch?v=TJhVotrZt9I
https://www.youtube.com/watch?v=x_uAtZlKllA
Slideshare link to NMR
https://www.slideshare.net/AFSATH/nmr-spectroscopy-78844956
https://www.slideshare.net/devishatatineni/nuclear-magnetic-resonance-spectroscopy-59592003
Session 42
Topic: Electron Paramagnetic Resonance (EPR)
Video on EPR
https://www.youtube.com/watch?v=73k30SNVgt8
https://www.youtube.com/watch?v=KOnodnj8a_8
https://www.youtube.com/watch?v=AxlbjSROxwA
Slideshare link to EPR
https://www.slideshare.net/masreshaamare/masresha-epr-spectroscopy
Session 43
Topic: Principle and Application of Fully Automated Biochemistry Analyser
Video on Fully Automated Biochemistry Analyser
https://www.youtube.com/watch?v=LHweSWdoQ78
Session 44
Practice session
Topic: Demonstration of Fully automated Analyzer
Video on Demonstration of Fully automated Analyzer
http://labsforlife.in/InstructionalVideo.aspx
https://www.youtube.com/watch?v=ksCjG_4MXUE
Session 45
Topic: Semi- automated Biochemistry Analyser
Video on Semi- automated Biochemistry Analyser
Session 46
Practice session
Topic: Demonstration of Semi automated Analyzer
Video on Demonstration of Semi automated Analyzer
http://labsforlife.in/InstructionalVideo.aspx
https://www.youtube.com/watch?v=b69HryqioM0
Session 47
Case Studies
Case Studies
Our Main Teachers
Dr. Soumya Jal is working at Centurion University of Technology and Management since 2018, prior to which she was associated with Rama Devi Women’s University as a visiting faculty. She has completed her Ph. D. in Medical Biotechnology from VIT University, Vellore (2016). Her research area includes fields such as, Applied and Clinical Microbiology, Molecular […]