Biochemistry and Metabolic Processes
Teacher
Sunita Satapathy
Category
Core Courses
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Course Name : Biochemistry & Metabolic Processes
Code(Credit) : CUTM1506(3-2-1)
Course Objectives
- To know the functioning of various organs and their inter relationship.
- To make students to know about the various metabolic processes
Coursc Outcomcs
- Students will acquire the knowledge of functioning of different body parts.
- The students can apply the acquired knowledge for higher study.
| COs | Course Outcomes | Mapping COs with POs
(High-3, Medium-2, Low-1) |
| CO1 | Student should be able to describe unique characters of urochordates, cephalochordates and fishes.
|
PO1-3, PO2-1, PO9-2 |
| CO2 | Student should be able to recognize life functions of urochordates to fishes. | PO1-3, PO2-3, PO9-2 |
| CO3 | Acquire in depth knowledge on the diversity of chordates and their systematic position | PO1-3, PO2-2, PO9-2 |
Course Syllabus
MODULE- I
Overview of Metabolism :
1.1- Stages of Metabolism (Catabolism vs Anabolism)
1.2- Compartmentalization of metabolic pathways,
1.3-ATP as "Energy Currency of cell" & Use of reducing equivalents and cofactors
1.4-Intermediary metabolism and regulatory mechanisms
Practice-1
To demonstrate the effect of temperature on enzyme activity.
Assignment-1-a) Intermediary metabolism and regulatory mechanisms
MODULE- II
Carbohydrate Metabolism :
2.1-Sequence of reactions and regulation of glycolysis & Gluconeogenesis
2.2-Citric acid cycle and significance
2.3-Phosphate Pentose Pathway (Role and significance)
2.4-Glycogenesis
2.5-Glycogenolysis
Practice-2
To estimate the carbohydrate content of supplied tissue by Spectrophotometry method
Practice-3
To estimate the glycogen content of supplied tissue by colorimeter method
Assignment-2 -a) Significance of Glycolysis, b) Role of Phosphate Pentose Pathway, c) Glycogenesis-role of UDP-Glucose-PPT
MODULE- III
3. Lipid Metabolism:
3.1-β-oxidation of saturated fatty acids with even & odd number of carbon atoms
3.2- Omega -oxidation of even & odd number of saturated fatty acids
3.3-Biosynthesis of palmitic acid
3.4-Metaboliosm of unsaturated fatty acid
3.5-Ketogensis
3.6-Metabolic Fuel Utilization & Regulation of fatty acid
Practice-4
To estimate the lipid content from the given sample
Practice-5
Dry Lab: To trace the labelled C atoms of Acetyl-CoA till in the TCA cycle
Assignment-3 -a) Beta -Oxidation, b)Metaboliosm of unsaturated fatty acid
MODULE- IV
Protein Metabolism:
4.1-Catabolism of amino acids
4.2-Transamination
4.3-Deamination
4.4-Protein Metabolidsm
Practice-5
To estimate the protein content of supplied tissue by colorimeter method
Practice-6
Estimation of total protein content in the given sample by Lowry’s /Barfoed's Method.
Practice-7
To estimate amino acids from a mixture by using chromatography (paper/Thin layer)
Assignment-4- a) Role of aminotransferease, b)Protein Metabolism-PPT
MODULE- V
Fate of carbon skeleton
5.1-Urea cycle
5.2-Fate of C-skeleton of Glucogenic & Ketogenic amino acids
5.3-Regulation of Urea cycle
5.4-Role of mitochondria
Practice-8
Detection of SGOT in given sample using available kit. (Chick/Goat Serum
Practice-9
Determination of SGPT in given sample by using available kit.(Chick/Goat Serum)
Assignment-5 a) Role of mitochondria in urea cycle, b) Role of enzymes of urea cycle
MODULE- VI
Oxidative Phosphorylation
6.1-Oxidative Phosphorylation
6.2-Redox systems & Coupled reactions
6.3-Review of mitochondrial respiratory chain
6.4-Shuttle systems & Membrane transporters
Practice-10
Estimation of known and unknown protein
Practice-11
Preparation of Standard Curve of Bovine serum Albumin
Assignment-6 a) Role of ETS ,b) Shuttle system-PPT/e-assigned note
MODULE- VII
Inhibitors and Uncouplers
7.1-Inhibitors of Electron Transport System
7.2-Uncouplers of Electron Transport System
7.3-Activity of Enzyme
Practice-12
Study the enzymatic activity of Trypsin and Lipase
Session Plan
Session-1
Session 2
1.2-Compartmentalization of metabolic pathways
Session 3
1.3-ATP as "Energy Currency of cell" and Use of reducing equivalents and cofactors,Couple reaction
Session 4
1.4-Intermediary metabolism and regulatory mechanisms
Session 5
Practice-1 -To demonstrate the effect of temperature on enzyme activity
Session 6
Assignment-1- a)Types of molecules involve in metabolism, b)Notes on ATP, c)Intermediary reactions
Session 7
Flip class 1- Role of ATP on metabolic reaction
Session-8
2.1 -Sequence of reactions and regulation of Glycolysis & Gluconeogenesis
Session 9
2.2-Citric acid cycle and significance
Citric acid cycle and significance-1
Session 10
Session 11
Session 12
Session 13
Session 14
Session 15
Assignment-2 -a) Significance of Glycolysis, b) Role of Phosphate Pentose Pathway, c) Glycogenesis-role of UDP-Glucose-PPT
Session 16
Flip Class:2--Citric acid cycle-3
Session 17
3.1-β-oxidation of saturated fatty acids with even number of carbon atoms
Session 18
3.2-Omega -oxidation of saturated fatty acids with even and odd number of carbon atoms
Session 19
Session 20
Session 21
Session 22
3.6--Metabolic Fuel Utilization & Regulation of fatty acid
Session 23
Practice-4
To estimate the lipid content from the given sample
Session 24
Assignment-3-Dry run of Beta Oxidation of Fatty Acid-PPT/Programming
Session 25
Flip Class :3-Beta oxidation
Session 26
4.1-Catabolism of amino acids
Session 27
4.2-Transamination & it's regulation
Session 28
Session 30
Practice-5
To estimate the protein content of supplied tissue by colorimeter method
Session 31
Practice-6
Estimation of total protein content in the given sample by Lowry’s /Barfoed's Method.
Session 32
Practice-7
To estimate amino acids from a mixture by using chromatography (paper/Thin layer)
Session 33
Assignment-4- a) Role of aminotransferease, b)Protein Metabolism-PPT
Session 34
Flip Class -4-Protein metabolism
Session 37
5.3-Regulation of Urea cycle
Session 38
Session 39
Practice-8
Estimation of Bile/Sugar from Urine sample
UrineEstimation-Sugar-Urine-2-
Session 40
Session 41
Assignment-5 a) Role of mitochondria in urea cycle, b) Role of enzymes of urea cycle
Session 42
Session 43
Session 44
6.3-Review of mitochondrial respiratory chain
Session 45
6.4-Shuttle systems & Membrane Transporters
Membrane Transporters
Session 46
Session 47
Session 48
Assignment-6 a) Role of ETS ,b) Shuttle system-PPT/e-assigned note
Session 49
Topic-7.1-Inhibitors of Electron Transport System
Session -50
Session -51
Session 52
Practice-12
Study the enzymatic activity of Trypsin and Lipase
- Reference
E-content:
Text Books:
- Cox, M.M and NelsoEXT n, D.L. (2008). Lehninger’s Principles of Biochemistry, V Edition, W.H. Freeman and Co., New York.
Reference Books:
- Cox, M.M and NelsoEXT n, D.L. (2008). Lehninger’s Principles of Biochemistry, V Edition,
- W.H. Freeman and Co., New York.
- Berg, J.M., Tymoczko, J.L. and Stryer, L. (2007). Biochemistry, VI Edition, W.H. Freeman and Co., New York.rs
- Sources of Environmental hazards, hazard identification and accounting, fate of toxic and persistent substances in the environment, dose Response Evaluation, exposure Assessment.
Case Studies
Case Studies
Our Main Teachers
Experienced with teaching profession in the field of Biochemistry,Animal Physiology, Cell biology. Presently working as Assistant Professor in Department of Zoology,School of Applied Science,Centurion University of Technology & Management, Odisha, India. Pursuing P.hD in Vermitechnology (recycling Biodegradable Wastes ) in CUTM. Received M.Phil in Biotechnology on Activity of Enzyme and M.Sc. in BIOCHEMISTRY as Specialisation […]