Introduction to biotechnology and Bioinformatics
Teacher
Mr Zahoor Mushtaq
Category
Core Courses
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Course Name : Introduction to biotechnology and Bioinformatics
Code(Credit) : FSRM 3210(1-1-0)
Course Objectives
- To understand basic biotechnological concepts and biotechnological applications for enhancing production through sustainable eco-friendly culture.
- To understand basic bioinformatics and various in silico approches used in the field of biotechnology
Course Outcomes
CO1: To understand the fundamentals of biotechnology and bioinformatics:PO1,PO2
CO2:To understand various molecular techniques for species identification, gene manipulation and immunological aspects for aquaculture:PO1,PO2
CO3:To demonstrate the applications of biological databases for molecular visualization and sequence analysis:PO1,PO2,PO6
PO1:Knowledge enhancement: Enhanced knowledge on concepts, essentialfacts, basics, principles and theories relating to fishery biology, biodiversity and aquatic environment, aquaculture, fish nutrition, fish physiology, genetics and biotechnology, fish processing, fisheries engineering, fish microbiology, fishery extension, economics, and biostatistics.
PO2:Professional and Entrepreneurial Skill development: Understanding the value and processes of life-long learning and professional enhancement and entrepreneurship development.
PO6:Data Handling and computational skills: Collect, analyze and interpret biological and scientific data for the scientific and farming community with the help of computer-based programmes.
Course Syllabus
Theory
Biotechnology: Introduction to Biotechnology –scope and importance in fisheries/aquaculture; Structural organization of prokaryotic and eukaryotic cell. Nucleic acids -structure, function and types, Concepts of gene and genetic code, transcription and translation, mutations and their implications. Post transcriptional modification and RNA processing. Gene regulation and expression in prokaryotes and eukaryotes; DNA sequencing, Operons. Genetic engineering- Restriction enzymes; Gene isolation; Cloning vectors; Probes; Recombinant DNA technology – vaccines. Transgenic fish and Gene transfer technology, Animal Cell Culture, Hybridoma technology. Molecular and immunological techniques – PCR; immunoblotting; ELISA; Principle of hybridization; Northern blotting; Western blotting; Southern blotting; DNA fingerprinting; Restriction fragment length polymorphism., Biosensors. Concept of bioremediation of water, bioprocess engineering and bioprospecting.Genomics
Bioinformatics: Introduction to Bioinformatics; Biological Databases and tools :Introduction; Types of biological databases; Primary and secondary databases; PDB, NCBI, formats and contents; Sequence retrieval, manipulation; Primer design; Restriction mapping; ORF finding; EMBOSS, Molecular visualization Sequence analysis.
Practicals
Study of structure of prokaryot and Eukaoryt Cells. Study on Model of protein Synthesis, Study of models rDNA Technology, Cell Culture, Isolation of Nucleic Acids, Restriction enzymes, Gel Electrophorus, ELISA, DNA sequence analysis and comparison.
Suggested Reading
Fish Genetics & Biotechnology ---- W.S.Lakra
Fish Biotechnology-Ranga et al,2002
Bioinformatics for beginers-Amjesh and Vinodchandra,2019
Session Plan
Session 1 (Theory)
Introduction to Biotechnology –scope and importance in fisheries/aquaculture
Download PPTs:Download PPT1 Th. Introduction to biotechnology
Download PPT2: INTRODUCTION TO BIOTECHNOLOGY
Video link1:https://www.youtube.com/watch?v=D9Jlc5ESqFE
Video link2: https://www.youtube.com/watch?v=u23UEqoJzRM
Session 2 (Theory)
Structural organisation of prokaryotic and eukaryotic cell
Download PPT:cell organisation
Video link:Plant and animal cell
Session 3 (Theory)
Nucleic acids -structure, function and types.
Download PPT1: Nucleic acid structure
Download PPT2:Nucleic acids
Video link:https://www.youtube.com/watch?v=0lZRAShqft0
Session 4 (Theory)
Concepts of gene and genetic code, transcription and translation, mutations and their implications.
Downoad PPT1:Transcription-and-translation
Download PPT2:genetic Code
Video link:https://www.youtube.com/watch?v=215d0eiO71w
Session 5 (Theory)
Post transcriptional modification and RNA processing. Gene regulation and expression in prokaryotes and eukaryotes, operons.
Download PPT:RNA-processing-and-gene-regulation
Video link:https://www.youtube.com/watch?v=zEfbELSNqzA
Session 6 (Theory)
Genetic engineering- Recombinant DNA technology, Restriction enzymes; Gene isolation; Cloning vectors; Probes; DNA sequencing and genomics.
Download PPT:Genetic-engineering-and-genomics
Video link:https://www.youtube.com/watch?v=ZW9zPdb_Bs0
Session 7 (Theory)
Transgenic fish and gene transfer technology
Download PPT1:Transgenic-fish-and-gene-transfer
Download PPT2:transgenic fish
Video link:https://www.youtube.com/watch?v=RzYhcXjksKc
Session 8 (Theory)
Vaaxines, Types and process of vaccination
Download PPT1:Vaccines and types
Download PPT2: DNA VACCINES
Video link:https://www.youtube.com/watch?v=u33Xq-4IY20
Session 9 (Theory)
Animal cell culture
Download PPT1:Animal-Cell-Culture.
Video link:https://www.youtube.com/watch?v=nr1tV_LuqJk
Session 10 (Theory)
Hybridoma technology and production of monoclonal antibodies.
Download PPT:Hybridoma-Technology-and-mAbs
Video link:https://www.youtube.com/watch?v=8iyrbv1JauY
Session 11 (Theory)
Molecular techniques nucleic acid based, PCR and hybridisation
Download PPT:Molecular-techniques
Video link:https://www.youtube.com/watch?v=uKeMiAZ8Zu4
Session 12 (Theory)
Immunological techniques. ELISA
Download PPT1:Immunological-techniques
Video link:https://www.youtube.com/watch?v=YJ0-qQslqqQ
Session 13 (Theory)
Biosensors. Concept of bioremediation of water, bioprocess engineering and bioprospecting.
Download PPT1:Biosensors.-bioprocess-and-bioprospecting
Video link: https://www.youtube.com/watch?v=gJd0MeECLHA
Session 14 (Theory)
Session 15 (Theory)
Biological Databases
Download PPT1:Bioinformatics
Video link: https://www.youtube.com/watch?v=2rD3OWsVXTo
Session 16 (Theory)
Immunological techniques, western blotting
Download PPT1Immunological-techniques
Video link:https://www.youtube.com/watch?v=N8XbauUsns4
Session 17 (Practical)
Steps in conventional PCR.
Session 18 (Practical)
Overview of ELISA
Session 19 (Practical)
Polymerase Chain Reaction
Session 20 (Practical)
Bioprocess engineering and bioprospecting overview
Session 21 (Practical)
Study on model of protein synthesis
Session 22 (Practical)
Study on structure of eukaryotic cell
Session 23 (Practical)
Study on model of transcription
Session 24 (Practical)
Study on the model of translation
Session 25 (Practical)
Study on model of rDNA technology
Session 26 (Practical)
Isolation of DNA from fish tissue
Session 27 (Practical)
Isolation of RNA from fish tissue
Session 28 (Practical)
Isolation of plasmid DNA
Session 29 (Practical)
agarose gel electrophoresis of DNA
Session 30 (Practical)
Agarose gel electrophoresis of RNA
Session 31 (Practical)
Agarose gel electrophoresis of plasmid DNA
Session 32 (Practical)
Overview of NCBI/GenBank database
E-Content
Model Questions
Assignments
Our Main Teachers
Mr Zahoor Mushtaq is working as Assistant Professor in school of fisheries at Centurion University of Technology and management. He has completed BFSc from SKUAST-Kashmir and MFSc in Aquatic Animal Health Management from ICAR-CIFE, Mumbai. His research interests are fish pathology , immunology and molecular biology.