Diagnostic Mycology

Teacher

Dr. Monali Priyadarsini Mishra

Category

Core Courses

Course Name : CUTM1745-Diagnostic Mycology

Code(Credit) : (3-1-0)

Course Objectives

  • To confirm the suspicion of fungal disease.
  • To identify the etiologic agent by isolating the causative fungal pathogen.

Learning Outcomes

  • This course provides learning opportunities in the basic principles of medical microbiology and infectious fungal diseases.
  • The course provides the conceptual basis for understanding pathogenic fungi and the mechanisms by which they cause disease in the human body.
  • Student can safeguard himself & society and can work diagnostics and hospitals 

Course Syllabus

Module-1 

Diagnostic Mycology: Medically important fungi, Opportunistic Fungi

Fungal Diseases: Mycoses

  1. Superficial mycoses
  2. Subcutaneous mycoses
  3. Cutaneous mycoses: Trichophytosis, Microsporiosis, Epidermophytosis.
  4. Systemic mycoses: Histoplamosis, Blastomycosis, Cryptococcosis, Coccidioidosis, Paracoccidioidosis .

Practice:

  1. Antifungal Sensitivity Test: Antibiotics.
  2. Preparation of stock solution of drug

Module- 2 

Pathogenesis and Laboratory Diagnosis of Mycotic Infections: Aspergillus spp, Candida albicans, Fusarium spp, Cryptococcus neoformans, Histroplasma capsulatum, Sporothourixspp, Philophora spp., Trichophotonmicrosporium, Epidermphyton spp. Blastomyces dermastitidis, Coccidiodisimmitens.

Practice:

  1. Dilution technique
  2. Determination of Minimal inhibitory concentration (MIC)

Unit 3

Isolation and Identification of Fungi (Laboratory Diagnosis):A. Selection, collection and transportation of specimens 5 hours Skin, Hair, Nail, Mucous membranes, Ear, eye, Cornial ulcer, Pus, Blood, Biopsy, Sputum, Urine, Vaginal and Cervical swab, Stool samples, Plural and peritonial fluid, Superficial, sub-cutanious and cutanious samples.B. Smear Preparation: 2 hours KOH Preparation, 20% KOH with 20% Glycerol, KOH – DMSO (DimethyalSulphoxide) 100% Lactophenol Cotton Blue, India ink preparation.

Practice:

  1. Preparation of different media, chemical and stain for fungus study
  2. Isolation and identification of different fungi of medical important

Suggested References:

  1. Textbook of Microbiology- Ananthanarayan & Paniker (10th Ed)
  2. Medical Microbiology-by Fritz H. Kayser et al
  3. Fundamental medical mycology / Errol Reiss, H. Jean Shadomy, and G. Marshall Lyon III
  4. Essential Medical Microbiology- by Rajesh Bhatia (4th Ed)
  5. Clinical Microbiology Procedures Handbook- by Amy L. Leber (4th Ed)
  6. The short text book of medical microbiology- by Satis Gupte (10th Ed)

Session Plan

Session 1

Introduction to Diagnostic Mycology: Medically important fungi

Video: https://www.youtube.com/watch?v=f7QxqWEKoGs

Presentatuion: https://www.slideshare.net/mgetso/introduction-to-medical-mycology

Session 2

Session 3

Session 4

Session 5

Session 6

Session 7

Session 8

Session 9

Session 10

Session 11

Practice:

Antifungal Sensitivity Test: Antibiotics.

Video: https://www.youtube.com/watch?v=coTMjdvuTY8

Video: https://www.youtube.com/watch?v=3Rw11wPbw4I

Practice:

Preparation of stock solution of drug

Video: https://www.youtube.com/watch?v=5RQbMDfc2BM

Session 12

Pathogenesis and Laboratory Diagnosis of Mycotic Infections: Aspergillus spp

Video: https://www.youtube.com/watch?v=N1UUazCiL78

Video: https://www.youtube.com/watch?v=NDrbHSIzcLI

Presentation: https://www.slideshare.net/rajhaviknesh/aspergillosis-eassignm

Session 13

Session 14

Session 15

Session 16

Session 17

Session 18

Session 19

Session 20

Session 21

Session 22

Practice:

Dilution technique

https://www.youtube.com/watch?v=Ppe_bgnPFHU

Practice:

Determination of Minimal inhibitory concentration (MIC)

https://www.youtube.com/watch?v=WAvRj1cRwXM

https://www.youtube.com/watch?v=MqRYC1m-v3c

Session 23

Session 24

Selection, collection and transportation of specimens from  Nail

Video: https://www.youtube.com/watch?v=WyyjhA5gG7M

Presentation: https://www.slideshare.net/albertgonzalobautista/2-specimen-collection

Session 25

Session 26

Session 27

Session 28

Session 29

Session 30

Session 31

Session 32

Session 33

Session 34

Session 35

Practice:

Preparation of different media, chemical and stain for fungus study

https://www.youtube.com/watch?v=FcW5tHrbAos

 

Practice:

Isolation and identification of different fungi of medical important

https://www.youtube.com/watch?v=GV840R4Pyi4&t=165s

Case Studies

Case Studies