Animal Biotechnology-CUTM2365, Credits (3-0-1)

Teacher

Dr Fahima Dilnawaz

Category

Core Courses

Course Attendees

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Course Reviews

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Course outline

To provide the fundamentals of animal cell culture, details of disease and therapy

Course outcome

This course deals with animal cell culture, embryo transfer, recombinant vaccines, lactation and milk production

Syllabus

  1. Module I

Animal Cell Culture

Introduction to basic tissue culture techniques; chemically defined and serum free media; animal cell cultures, their maintenance and preservation; various types of cultures; suspension cultures, continuous flow cultures, immobilized cultures;

Module II

Somatic cell fusion                                                                                             

Somatic cell fusion; cell cultures as a source of valuable products

Module III

Organ cultures                                                            

Information about organs; culture techniques and preservations

 

Module IV

 

Animal diseases and their Diagnosis                                                                                                       

 

Bacterial and viral diseases in animals; monoclonal antibodies and their use in diagnosis; molecular diagnostic techniques like PCR, in-situ hybridization; northern and southern blotting; RFLP.

 

Module V

Therapy of Animal Diseases                                                                                                      

Recombinant cytokines and their use in the treatment of animal infections; monoclonal antibodies in therapy; vaccines and their application in animal infections; gene therapy for animal diseases.

 

Module VI

Micromanipulation of Embryo’s

         What is micromanipulation technology; equipment’s used in micromanipulation; enrichment of x and y bearing sperms from semen samples of animals; artificial insemination and germ cell manipulation; invitro fertilization and embryo transfer; micromanipulation technology and breeding of farm animals.

 

Module VII

Transgenic Animals

Concepts of transgenic animal technology;strategies for the production of transgenic animals and their importance in biotechnology; stem cell cultures in the production of transgenic animals

 

  1. Reference

 

Text Books:

 

  1. Animal biotechnology by P.Ramadas
  2. Embryonic stem cells by Kursad and Turksen. 2002. Humana Press.

 

 Reference Books

  1. Louis-Marie Houdebine, Transgenic animals: Generation and Use 7th Edition, CRC Press.

 

 

 

Session Plan

Session-1

Introduction to basic tissue culture techniques, https://www.youtube.com/watch?v=OBagQf8_lZM

Session-2

Chemically defined and serum free media, https://www.youtube.com/watch?v=UV7T9JsxdXA

Session-3

Animal cell cultures, their maintenance and preservation,

 

Session-4

various types of cultures; suspension cultures https://www.youtube.com/watch?v=6Wdyyu-vQsU

https://www.youtube.com/watch?v=G1n3BgmcVMA

Session-5

Continuous flow cultures, immobilized cultures;

https://www.youtube.com/watch?v=ZNiCtceTvNc

https://www.youtube.com/watch?v=_e2V3Z_4f78

Session-6

 

Somatic cell fusion: cell cultures as a source of valuable products

https://www.youtube.com/watch?v=AGJHnoFucRs

Session-7

Organ cultures: Information about organs; culture

 

Session-8

Techniques and preservations

https://www.youtube.com/watch?v=noBMjQXmcjU

Session-9

Animal diseases and their Diagnosis: Bacterial diseases in animals

https://www.youtube.com/watch?v=yK0RjZCAfec

https://www.youtube.com/watch?v=Oax3GTZv4DI

 

Session-10

Viral diseases in animals

youtube.com/watch?v=7mdEfyK9r2M

Session-11

Monoclonal antibodies and their use in diagnosis

https://www.youtube.com/watch?v=fsraEA0E06o

Session-12

Molecular diagnostic techniques like PCR

https://www.youtube.com/watch?v=TtPEm3HT1Y0

 

Session-13

In-situ hybridization

https://www.youtube.com/watch?v=n6dN2pjCQuU

https://www.youtube.com/watch?v=dRmWZ3IUjFo

 

Session-14

Northern blotting

https://www.youtube.com/watch?v=k9mDO6NPUQ8

Session-15

Southern blotting

https://www.youtube.com/watch?v=XEWu78qNcIg

Session-16

Restriction fragment length polymorphism (RFLP)

 

Session-17

Recombinant cytokines

https://www.youtube.com/watch?v=-DW18Zoj8hs

https://www.youtube.com/watch?v=G8k17APYSv8

 

Session-18

Recombinant cytokines and their use in the treatment of animal infections;

 

Session-19

https://www.youtube.com/watch?v=bqJ8hbGcv-g

 

Session-20

Monoclonal antibodies in therapy

https://www.youtube.com/watch?v=dxnjAc-rqz8

 

Session-21

Vaccines and their application in animal infections

https://www.youtube.com/watch?v=huqmZ9D5P1U

https://www.youtube.com/watch?v=lYsLz1CsA4c

Session-22

Gene therapy for animal diseases

https://www.youtube.com/watch?v=hy-oQOuJBUM

Session-23

Micromanipulation of Embryo’s, Equipment’s used in micromanipulation

 

https://www.youtube.com/watch?v=Ehw4OxMcW0M, https://www.youtube.com/watch?v=W3T6EFkGRYM

Session-24

Enrichment of x and y bearing sperms from semen samples of animals

https://www.youtube.com/watch?v=wJc0SX03-VA

Session-25

Artificial insemination and germ cell manipulation

https://www.youtube.com/watch?v=stvnGYcKz60

https://www.youtube.com/watch?v=BfU558VJdIk

Session-26

Invitro fertilization and embryo transfer

https://www.youtube.com/watch?v=dtJ-fBt1TaQ

Session-27

Micromanipulation technology and breeding of farm animals.

https://www.youtube.com/watch?v=TwJLQR_P0IM

 

Session-28

Transgenic Animals, Concepts of transgenic animal technology

https://www.youtube.com/watch?v=RzYhcXjksKc

 

Session-29

Strategies for the production of transgenic animals and their importance in biotechnology

https://www.youtube.com/watch?v=ItAMrW4CX48

 

Session-30

Stem cell cultures in the production of transgenic animals

https://www.youtube.com/watch?v=yXSuq_3BqVE

 

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Our Main Teachers

Objective To provide depth knowledge of biological or medicinal processes through the investigation of the underlying molecular mechanisms. • Understanding of chemical and molecular processes that occur in and between cells.   •Understanding will become such that, can be able to describe and explain processes and their meaning for the characteristics of living organisms. Learning outcome […]