Course outline
To provide the fundamentals of animal cell culture, details of disease and therapy
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Course outline
To provide the fundamentals of animal cell culture, details of disease and therapy
Course outcome
This course deals with animal cell culture, embryo transfer, recombinant vaccines, lactation and milk production
Syllabus
Animal Cell Culture
Introduction to basic tissue culture techniques; chemically defined and serum free media; animal cell cultures, their maintenance and preservation; various types of cultures; suspension cultures, continuous flow cultures, immobilized cultures;
Module II
Somatic cell fusion
Somatic cell fusion; cell cultures as a source of valuable products
Module III
Organ cultures
Information about organs; culture techniques and preservations
Module IV
Animal diseases and their Diagnosis
Bacterial and viral diseases in animals; monoclonal antibodies and their use in diagnosis; molecular diagnostic techniques like PCR, in-situ hybridization; northern and southern blotting; RFLP.
Module V
Therapy of Animal Diseases
Recombinant cytokines and their use in the treatment of animal infections; monoclonal antibodies in therapy; vaccines and their application in animal infections; gene therapy for animal diseases.
Module VI
Micromanipulation of Embryo’s
What is micromanipulation technology; equipment’s used in micromanipulation; enrichment of x and y bearing sperms from semen samples of animals; artificial insemination and germ cell manipulation; invitro fertilization and embryo transfer; micromanipulation technology and breeding of farm animals.
Module VII
Transgenic Animals
Concepts of transgenic animal technology;strategies for the production of transgenic animals and their importance in biotechnology; stem cell cultures in the production of transgenic animals
Text Books:
Reference Books
Session Plan
Session-1
Introduction to basic tissue culture techniques, https://www.youtube.com/watch?v=OBagQf8_lZM Session-2 Chemically defined and serum free media, https://www.youtube.com/watch?v=UV7T9JsxdXA Session-3 Animal cell cultures, their maintenance and preservation,
Session-4 various types of cultures; suspension cultures https://www.youtube.com/watch?v=6Wdyyu-vQsU https://www.youtube.com/watch?v=G1n3BgmcVMA Session-5 Continuous flow cultures, immobilized cultures; https://www.youtube.com/watch?v=ZNiCtceTvNc https://www.youtube.com/watch?v=_e2V3Z_4f78 Session-6
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Somatic cell fusion: cell cultures as a source of valuable products
https://www.youtube.com/watch?v=AGJHnoFucRs
Session-7
Organ cultures: Information about organs; culture
Session-8
Techniques and preservations
https://www.youtube.com/watch?v=noBMjQXmcjU
Session-9
Animal diseases and their Diagnosis: Bacterial diseases in animals
https://www.youtube.com/watch?v=yK0RjZCAfec
https://www.youtube.com/watch?v=Oax3GTZv4DI
Session-10
Viral diseases in animals
youtube.com/watch?v=7mdEfyK9r2M
Session-11
Monoclonal antibodies and their use in diagnosis
https://www.youtube.com/watch?v=fsraEA0E06o
Session-12
Molecular diagnostic techniques like PCR
https://www.youtube.com/watch?v=TtPEm3HT1Y0
Session-13
In-situ hybridization
https://www.youtube.com/watch?v=n6dN2pjCQuU
https://www.youtube.com/watch?v=dRmWZ3IUjFo
Session-14
Northern blotting
https://www.youtube.com/watch?v=k9mDO6NPUQ8
Session-15
Southern blotting
https://www.youtube.com/watch?v=XEWu78qNcIg
Session-16
Restriction fragment length polymorphism (RFLP)
Session-17
Recombinant cytokines
https://www.youtube.com/watch?v=-DW18Zoj8hs
https://www.youtube.com/watch?v=G8k17APYSv8
Session-18
Recombinant cytokines and their use in the treatment of animal infections;
Session-19
https://www.youtube.com/watch?v=bqJ8hbGcv-g
Session-20
Monoclonal antibodies in therapy
https://www.youtube.com/watch?v=dxnjAc-rqz8
Session-21
Vaccines and their application in animal infections
https://www.youtube.com/watch?v=huqmZ9D5P1U
https://www.youtube.com/watch?v=lYsLz1CsA4c
Session-22
Gene therapy for animal diseases
https://www.youtube.com/watch?v=hy-oQOuJBUM
Session-23
Micromanipulation of Embryo’s, Equipment’s used in micromanipulation
https://www.youtube.com/watch?v=Ehw4OxMcW0M, https://www.youtube.com/watch?v=W3T6EFkGRYM
Session-24
Enrichment of x and y bearing sperms from semen samples of animals
https://www.youtube.com/watch?v=wJc0SX03-VA
Session-25
Artificial insemination and germ cell manipulation
https://www.youtube.com/watch?v=stvnGYcKz60
https://www.youtube.com/watch?v=BfU558VJdIk
Session-26
Invitro fertilization and embryo transfer
https://www.youtube.com/watch?v=dtJ-fBt1TaQ
Session-27
Micromanipulation technology and breeding of farm animals.
https://www.youtube.com/watch?v=TwJLQR_P0IM
Session-28
Transgenic Animals, Concepts of transgenic animal technology
https://www.youtube.com/watch?v=RzYhcXjksKc
Session-29
Strategies for the production of transgenic animals and their importance in biotechnology
https://www.youtube.com/watch?v=ItAMrW4CX48
Session-30
Stem cell cultures in the production of transgenic animals
https://www.youtube.com/watch?v=yXSuq_3BqVE
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Objective To provide depth knowledge of biological or medicinal processes through the investigation of the underlying molecular mechanisms. • Understanding of chemical and molecular processes that occur in and between cells. •Understanding will become such that, can be able to describe and explain processes and their meaning for the characteristics of living organisms. Learning outcome […]