Skill Course : Bio-Fertilizer Preparation


Dr. Praveen Boddana


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Course Name : Bio Fertilizer Preparation

Code(Credit) :           (0-3-1)

Course Objectives

  • To impart hands on training on the skills associated with Biofertilizer organisms isolation ,production and application.

Learning Outcomes

  • Students will be acquiring  the technical knowledge in Bio fertilizer production  technology 

Course Syllabus


  • Module 1 :Hands on training on equipment in Biofertilizer unit
  • Module 2: Isolation and purification of Azospirillum .
  • Module 3: Isolation and purification of Azotobacter.
  • Module 4: Isolation and purification of Rhizobium.
  • Module 5: Isolation and purification of P-solubilizers 
  • Module 6: Isolation and purification of Cyanobacteria.
  • Module 7: Isolation of AM fungi -Wet sieving method and sucrose gradient method.
  • Module 8: Mass multiplication of Biofertilizers.

Reference books :

1.A Text Book of Microbiology-R.C.Dubey and Maheswari.

2.Handbook of Biopesticides and Biofertilizers-A.M.Deshmukh,R.M.Khobragade,P.P.Dixit.

Session Plan

Session 1

.Hands on training on equipment in Bio fertilizer unit and Lab set up

Biofertilizer unit.

Session 2

.Isolation of Azospirillum

Biofertilizer practice

Session 3

.Isolation of Nirogen fixing bacteria


Session 4

.Isolation of Rhizobium


Session 5

.Isolation of PSB(Phospahte solubilizing bacteria)


Session 8

Mass multiplication of Biofertilizers


Biofertilizer practice


Mass multiplication of Biofertilizers and their marketing

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Case Studies

Our Main Teachers

    I am an experienced Bio-Medical Science Professional with 15 years of experience and strong academic background, having completed Masters and Doctorate in Biotechnology. Currently working as Associate Professor & Head Department of Plant Pathology,Centurion University Parlakhemundi,Odisha India (July 2016 – till date). My areas of interests include Microbiology, Immunology and Industrial Biotechnology. Major […]

Dr. Aravind. T

Assistant Professor

Dr. Aravind. T is working as Asst. Professor in Department of Plant Pathology, M.S. Swaminathan School of Agriculture, Centurion University of Technology and Management, India. He completed his graduation from Kerala Agriculture University, Kerala, Post-graduation from Anand Agriculture University, Gujarat and Ph. D from G. B. Pant University of Agriculture and Technology, Uttarakhand India. He […]