Principles of Biotechnology

Teacher

Dr. Panchashree Das

Category

Minor and supporting course

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Course name- Principles of Biotechnology

 

Code (Credit)- MMBB0501

 

Course Credit- 3+0

Course Objectives-

  1. To understand the basics of Molecular biology, plant and microbial Biotechnology.
  2. Importance and applications in agriculture, case studies and success stories.
  3. Public education, perception, IPR and related issues

CO-PSO mapping table-

 

COs

Course Outcomes

Mapping Cos with PSOs

CO1 Apply knowledge of genes, genomes and their organization, gene expression, genetic variation, genetic engineering, protein expression, and post-translational modifications. PSO1
CO2 Impart knowledge and understanding of fundamental concepts and techniques of crop improvement through biotechnological research. PSO1, PSO3
CO3 Compare various biotechnological approaches and recommend appropriate strategies for problem solving in a sustainable way.

 

PSO1, PSO3

 

PSO1 : Impart knowledge and understanding of fundamental concepts and techniques of maintaining and enhancing soil fertility, crop production, crop management, crop improvement, biodiversity and sustainability of agriculture worldwide.

PSO2 : Develop the ability to know farming practices and their scope to improve in the rural areas and the environments.

PSO3 : Apply knowledge of basic science through Agricultural microbiology, Plant biochemistry and Biotechnology.

PSO4 : Apply knowledge of Agri-business management and finance to develop profitable agricultural system.

Course Syllabus-

 

Unit I

History, scope and importance of Biotechnology; Specializations in Agricultural Biotechnology: Genomics, Genetic engineering, Tissue Culture, Bio-fuel, Microbial Biotechnology, Food Biotechnology etc. Basics of Biotechnology, Primary metabolic pathways, Enzymes and its activities.

Unit II

Structure of DNA, RNA and protein, their physical and chemical properties. DNA function: Expression, exchange of genetic material, mutation. DNA modifying enzymes and vectors; Methods of recombinant DNA technology; Nucleic acid hybridization; DNA/RNA libraries; Applications of gene cloning in basic and applied research, Plant transformation: Gene transfer methods and applications of GM crops.

Unit III

Molecular analysis of nucleic acids -PCR and its application in agriculture and industry, Introduction to Molecular markers: RFLP, RAPD, SSR, SNP etc, and their applications; DNA sequencing, different methods; Plant cell and tissue culture techniques and their applications.Introduction to genomics, transcriptomics, ionomics, metabolomics and proteomics. Plant cell and tissue culture techniques and their applications.

Unit IV

Introduction to Emerging topics: Genome editing, gene silencing, Plant microbial interactions,Success stories in Biotechnology, Careers and employment in biotechnology. Public perception of biotechnology; Bio-safety and bioethics issues; Intellectual property rights in biotechnology.

Suggested Readings-

 

1.Watson, J. D., Baker, T. A., Bell, S. P., Gann, A., Levine, M & Losick R (2014) Molecular Biology of the Gene, 7th edition, Cold Spring Harbor Laboratory Press, New York.

2. Brown, T. A. (2010) Gene Cloning and DNA analysis an Introduction 6th edition, Wiley Blackwell.

3. Primrose, S. B. and Twyman, R. (2006) Principles of gene Manipulation 7th edition, Wiley Blackwell.

4. Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India

Theory Session Plan-

Sessions Topics to be cover Book Online materials
1 History of Biotechnology Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/Mhsrg18XHFc
2 Scope and importance of Biotechnology Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/PhgzkMDifvo
3 Specializations in Agricultural Biotechnology Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/rxdlC_3_J4s
4 Genomics Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/BoUS65mxnIE
5 Genetic engineering Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/S0JfVd-DhGw
6 Tissue Culture Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/Hf6SsiPn7_U
7 Bio-fuel Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/OJw6WFkTPZo
8 Microbial Biotechnology Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/QtvohxRIZFI
9 Food Biotechnology Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/o-0kk9csAbk
10 Basics of Biotechnology Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/JePcbKiZaoQ
11 Primary metabolic pathways Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/ohbDUJejmqc
12 Enzymes and its activities Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/a5TMHlRxwFA
13 Structure and properties of DNA Watson, J. D., Baker, T. A., Bell, S. P., Gann, A., Levine, M &Losick R (2014) Molecular Biology of the Gene, 7th edition, Cold Spring Harbor Laboratory Press, New York https://youtu.be/-GVUPFunZFs
14 Structure and properties of RNA Watson, J. D., Baker, T. A., Bell, S. P., Gann, A., Levine, M &Losick R (2014) Molecular Biology of the Gene, 7th edition, Cold Spring Harbor Laboratory Press, New York https://youtu.be/Rcbx5znKo_8
15 Structure and properties of protein Watson, J. D., Baker, T. A., Bell, S. P., Gann, A., Levine, M &Losick R (2014) Molecular Biology of the Gene, 7th edition, Cold Spring Harbor Laboratory Press, New York https://youtu.be/PPJ7C3hcnPw
16 DNA function: Expression, exchange of genetic material Watson, J. D., Baker, T. A., Bell, S. P., Gann, A., Levine, M &Losick R (2014) Molecular Biology of the Gene, 7th edition, Cold Spring Harbor Laboratory Press, New York https://youtu.be/7tLV20dk-FM
17 Mutation Brown, T. A. (2010) Gene Cloning and DNA analysis an Introduction 6th edition, Wiley Blackwell https://youtu.be/vl6Vlf2thvI
18 DNA modifying enzymes and vectors Brown, T. A. (2010) Gene Cloning and DNA analysis an Introduction 6th edition, Wiley Blackwell https://youtu.be/oMgZtuFwfcs
19 Methods of recombinant DNA technology Brown, T. A. (2010) Gene Cloning and DNA analysis an Introduction 6th edition, Wiley Blackwell https://youtu.be/OpU_CQ0pFyQ
20 Nucleic acid hybridization Brown, T. A. (2010) Gene Cloning and DNA analysis an Introduction 6th edition, Wiley Blackwell https://youtu.be/29EC7L3t19c
21 DNA/RNA libraries Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/JQByjprj_mA
22 Applications of gene cloning in basic and applied research Brown, T. A. (2010) Gene Cloning and DNA analysis an Introduction 6th edition, Wiley Blackwell https://youtu.be/iWpGjeGz_r8
23 Introduction to Plant transformation Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/9_e3tLXd1yc
24 Vector less method of Plant Transformation Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/9_e3tLXd1yc
25 Agrobacterium-mediated Plant Transformation Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/yesNHd9h8k0
26 Applications of GM crops Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/kZIYkYNpnP0
27 Molecular analysis of nucleic acids Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/7lIyi57Gx2Q
28 Introduction to PCR Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/8rAgLPb85N0
29 Applications of PCR in agriculture and industry Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/8rAgLPb85N0
30 Introduction to Molecular markers Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/T-jteJDyz9Y
31 RFLP Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/swQ_pm4cR5o
32 RAPD Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/i5_ecgyY3dY
33 SSR Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/iGN2tFCLPZ0
34 SNP Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/lC7kZOOYFpo
35 Applications of Molecular Marker Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/T-jteJDyz9Y
36 Different methods of DNA sequencing Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/QcVK6Dr9aK4
37 Plant cell and tissue culture techniques and their applications Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/dFrx-t5J0PA
38 Introduction to genomics, transcriptomics, ionomics, metabolomics and proteomics. Primrose, S. B. and Twyman, R. (2006) Principles of gene Manipulation 7th edition, Wiley Blackwell https://youtu.be/0mqz94bRma8
39 Plant cell and tissue culture techniques Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/dFrx-t5J0PA
40 Application of Plant Tissue Culture Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/dFrx-t5J0PA
41 Introduction to Genome editing Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/2pp17E4E-O8
42 Introduction to gene silencing Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/cK-OGB1_ELE
43 Introduction to Plant microbial interactions Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/Jp6fQnIHwbo
44 Success stories in Biotechnology Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/0LKmP_ePWrA
45 Careers and employment in biotechnology Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/0LKmP_ePWrA
46 Public perception of biotechnology Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/0LKmP_ePWrA
47 Bio-safety and bioethics issues Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/E171qNrEesc
48 Intellectual property rights in biotechnology Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India https://youtu.be/4PzOdMSvxW0

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