Pharmaceutical microbiology (Practical)

Course Objectives

Study of all categories of microorganisms especially for the production of alcohol antibiotics, vaccines, and vitamins enzymes and understand the importance of sterilization, sterility testing of pharmaceuticals. Also learn the practical skill of isolation, identification and preservation of pure cultures along with the biochemical characterization.

CO PO Mapping

CO	Statements	PO MAPPING
CO-1	Understand methods of identification, isolation and preservation of various microorganisms	PO1, PO6
CO-2	To understand the importance and implementation of sterilization in pharmaceutical processing and industry	PO1, PO6, PO10, PO11
CO-3	Learn sterility testing of pharmaceutical products.	PO1, PO10, PO11
CO-4	Carried out microbiological assay techniques.	PO1, PO5, PO10
CO-5	To acquire and demonstrate competency in laboratory safety and in routine and specialized microbiological laboratory skills applicable to microbiological research or clinical methods, including accurately reporting observations and analysis.	PO1, PO5, PO11

Course Syllabus

1. Introduction and study of different equipments and processing, e.g., B.O.D. incubator,
laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator,
microscopes used in experimental microbiology.
2. Sterilization of glassware, preparation and sterilization of media.
3. Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations.
4. Staining methods- Simple, Grams staining and acid fast staining (Demonstration with
practical).
5. Isolation of pure culture of micro-organisms by multiple streak plate technique and other
techniques.
6. Microbiological assay of antibiotics by cup plate method and other methods
7. Motility determination by Hanging drop method.
8. Sterility testing of pharmaceuticals.
9. Bacteriological analysis of water
10. Biochemical test

Session Plan

Experiment- 1

1. Introduction and study of different equipments and processing, e.g., B.O.D. incubator,laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator,microscopes used in experimental microbiology.

1.Tools in Microbiology Laboratory 1.Tools in Microbiology Laboratory-converted

https://www.youtube.com/watch?v=2hDuPEHNOAY&ab_channel=MicrobiologyEasyNotes