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1.Introduction, history of microbiology, its branches, scope and its
importance. Introduction to Prokaryotes and Eukaryotes.
2.Study of ultra-structure and morphological classification of bacteria, nutritional requirements, raw materials used for culture media and physical parameters for growth, growth curve, isolation and preservation methods for pure cultures, cultivation of anaerobes, quantitative measurement of bacterial growth (total & viable count).
3.Study of different types of phase constrast microscopy, dark field
microscopy and electron microscopy.
1.Identification of bacteria using staining techniques (simple, Gram’s &Acid
fast staining) and biochemical tests (IMViC).
2.Study of principle, procedure, merits, demerits and applications of physical,
chemical gaseous,radiation and mechanical method of sterilization.
3.Evaluation of the efficiency of sterilization methods.
4.Equipments employed in large scale sterilization.
5.Sterility indicators.
1.Study of morphology, classification, reproduction/replication and
cultivation of Fungi and Viruses.
2.Classification and mode of action of disinfectants
Factors influencing disinfection, antiseptics and their evaluation. For
bacteriostatic and bactericidal actions.
3.Evaluation of bactericidal & bacteriostatic.
4.Sterility testing of products (solids, liquids, ophthalmic and other sterile
products) according to IP, BP and USP.
1.Designing of aseptic area, laminar flow equipments; study of different
sources of contamination in an aseptic area and methods of prevention,
clean area classification.
2.Principles and methods of different microbiological assay.
3.Methods for standardization of antibiotics, vitamins and amino acids.
4.Assessment of a new antibiotic.
1.Types of spoilage, factors affecting the microbial spoilage of
pharmaceutical products, sources and types of microbial contaminants,
assessment of microbial contamination and spoilage.
2.Preservation of pharmaceutical products using antimicrobial agents,
evaluation of microbial stability of formulations.
3.Growth of animal cells in culture, general procedure for cell culture, Primary, established and transformed cell cultures.
4.Application of cell cultures in pharmaceutical industry and research
Recommended Books (Latest edition)
1. W.B. Hugo and A.D. Russel: Pharmaceutical Microbiology, Blackwell Scientific
publications, Oxford London.
2. Prescott and Dunn., Industrial Microbiology, 4th edition, CBS Publishers & Distributors,
Delhi.
3. Pelczar, Chan Kreig, Microbiology, Tata McGraw Hill edn.
4. Malcolm Harris, Balliere Tindall and Cox: Pharmaceutical Microbiology.
5. Rose: Industrial Microbiology.
6. Probisher, Hinsdill et al: Fundamentals of Microbiology, 9th ed. Japan
7. Cooper and Gunn’s: Tutorial Pharmacy, CBS Publisher and Distribution.
8. Peppler: Microbial Technology.
9. I.P., B.P., U.S.P.- latest editions.
10. Ananthnarayan : Text Book of Microbiology, Orient-Longman, Chennai
11. Edward: Fundamentals of Microbiology.
12. N.K.Jain: Pharmaceutical Microbiology, Vallabh Prakashan, Delhi
13. Bergeys manual of systematic bacteriology, Williams and Wilkins- A Waverly company
Introduction and history of microbiology.
Branches of microbiology,its scope and importance.
Introduction to prokaryotes and eukaryotes,nutritional requirement of bacteria.
Study of structure and morphological classification of bacteria
Raw materials used for culture media and physical parameters for growth.
Quantitative measurement of bacterial growth (total & viable count)
Phase contrast microscopy and dark field microscopy.
Identification of bacteria using staining techniques(gram staining,simple staining)
Principle procedure, merit, demerit, and application of physical,chemical and gaseous methods of sterilization.
Principle procedure, merit, demerit, and application of radiation and mechanical methods of sterilization.
Classification and mode of action of disinfectants
Factors affecting disinfection,antiseptic and their evaluation for bacteriostatic and bactericidal actions.
Evaluation of bactericidal and bacteriostatic.
Design of aseptic area,laminar flow equipment.
Study of different sources of contamination in an aseptic area and methods of prevention.
Principles and methods of different microbiological assay.
Methods for standardization of vitamins and amino acids.
Factors affecting the microbial spoilage of pharmaceutical products.
Factors affecting the microbial spoilage of pharmaceutical products.
Preservation of pharmaceutical products using antimicrobial agents
Evaluation of microbial stability of formulations
Application of cell cultures in pharmaceutical industry and research.
1. W.B. Hugo and A.D. Russel: Pharmaceutical Microbiology, Blackwell Scientific
publications, Oxford London.
2. Prescott and Dunn., Industrial Microbiology, 4th edition, CBS Publishers & Distributors,
Delhi.
3. Pelczar, Chan Kreig, Microbiology, Tata McGraw Hill edn.
4. Malcolm Harris, Balliere Tindall and Cox: Pharmaceutical Microbiology.
5. Rose: Industrial Microbiology.
6. Probisher, Hinsdill et al: Fundamentals of Microbiology, 9th ed. Japan
7. Cooper and Gunn’s: Tutorial Pharmacy, CBS Publisher and Distribution.
8. Peppler: Microbial Technology.
9. I.P., B.P., U.S.P.- latest editions.
10. Ananthnarayan : Text Book of Microbiology, Orient-Longman, Chennai
11. Edward: Fundamentals of Microbiology.
12. N.K.Jain: Pharmaceutical Microbiology, Vallabh Prakashan, Delhi
13. Bergeys manual of systematic bacteriology, Williams and Wilkins- A Waverly company
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