PRINCIPLES OF GENETICS

Teacher

Dr. Niranjan Kumar Chaurasia

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COURSE NAME: PRINCIPLES OF GENETICS

COURSE CODE: MGPB 0501 (2-1-0)

COURSE OBJECTIVES:

Understanding the basic concepts of inheritance of genetic traits, helping students to develop their analytical, quantitative, and problem-solving skills from classical to molecular genetics.

COURSE OUTCOMES (COs)/PROGRAMME SPECIFIC OUTCOMES (PSOs) MAPPING:

COs PSOs
CO-1: Knowledge of principles of inheritance and advances in molecular genetics PSO-1
CO-2: Solve the problems on Mendelian inheritance and chromosome mapping, extraction of DNA, PCR amplification, electrophoresis, etc. PSO-3

PROGRAMME SPECIFIC OUTCOMES (PSOs):

PSO1: Impart knowledge and understanding of fundamental concepts and techniques of maintaining and enhancing soil fertility, crop production, crop management, crop improvement, biodiversity, and sustainability of agriculture worldwide.

PSO3: Apply knowledge of basic science through Agricultural microbiology, Plant Biochemistry, and Biotechnology.

SYLLABUS (THEORY):

Beginning of genetics, early concepts of inheritance, Mendel’s laws; Discussion on Mendel’s paper, Chromosomal theory of inheritance; Multiple alleles, Gene interactions, Sex determination, differentiation and sex-linkage, Sex-influenced and sex-limited traits; Linkage-detection, estimation; Recombination and genetic mapping in eukaryotes, Somatic cell genetics, Extra chromosomal inheritance; Mendelian population, Random mating population, Frequencies of genes and genotypes, Causes of change and  Hardy-Weinberg equilibrium; Nature, structure and replication of the genetic material; Organization of DNA in chromosomes, Genetic code; Protein biosynthesis; Genetic fine structure analysis, Allelic complementation, Split genes, overlapping genes, Pseudogenes, Ontogenesis, Gene families and clusters; Regulation of gene activity in prokaryotes and eukaryotes; Molecular mechanisms of mutation, repair, and suppression; Bacterial plasmids, insertion (IS) and transposable (Tn) elements; Molecular chaperones and gene expression; RNA editing; Gene isolation, synthesis and cloning, genomic and cDNA libraries; PCR based cloning, positional cloning; Nucleic acid hybridization and immunochemical detection; DNA sequencing; DNA restriction and modification, Anti-sense RNA and ribozymes; Micro-RNAs (miRNAs); Genomics and proteomics; metagenomics; Transgenic bacteria and bioethics; Gene silencing; genetics of mitochondria and chloroplasts; Concepts of Eugenics, Epigenetics, Genetic disorders.

SYLLABUS (PRACTICAL):

Laboratory exercises in probability and chi-square; Demonstration of genetic principles using laboratory organisms; Chromosome mapping using a three-point test cross; Tetrad analysis; Induction and detection of mutations through genetic tests; DNA extraction and PCR amplification; Electrophoresis: basic principles and running of amplified DNA; Extraction of proteins and isozymes; Use of Agrobacterium-mediated method and Biolistic gun; Detection of transgenes in the exposed plant material; Visit to transgenic glasshouse and learning the practical considerations.

REFERENCES:

Benjamin A. Pierce (2012) Genetics: A Conceptual Approach (4th edition)

Singh B D. (2009). Genetics. Kalyani Publishers (2nd Revised Edition)

Gardner, E J & Snustad, D P. (1991). Principles of Genetics. John Wiley & Sons. 8th ed. 2006

Klug, W S and Cummings, M R. (2003). Concepts of Genetics, Peterson Edu.

Strickberger, M W. (2005). Genetics (III Ed). Prentice Hall, New Delhi, India

SESSION PLAN (THEORY):

SESSION-I: Beginning of genetics

A Historical Background of Genetics

SESSION-2: Early concepts of inheritance, Mendel’s laws, Discussion on Mendel’s paper

Concept of inheritance, Mendel's principles of inheritance

SESSION-3: Chromosomal theory of inheritance

Chromosomal Theory of Inheritance

SESSION-5: Sex determination, differentiation and sex-linkage, Sex-influenced and sex-limited traits

SESSION-4: Gene interactions, Multiple alleles

SESSION-6: Linkage-detection, estimation

SESSION-7: Recombination and genetic mapping in eukaryotes, Chiasmata and the time of crossing over

SESSION-8: Somatic cell genetics, Extra chromosomal inheritance

SESSION-9: Mendelian population, Random mating population, Frequency of genes and genotypes, Causes of change:  Hardy-Weinberg equilibrium.

SESSION-10: Nature, structure and replication of the genetic material

Nature, structure and replication

SESSION-11: Organization of DNA in chromosomes, Genetic code

Genetic code and translation

SESSION-12: Protein biosynthesis

Transcription_translation

SESSION-13: Genetic fine structure analysis, Allelic complementation,

SESSION-14: Split genes, overlapping genes, Pseudogenes, Ontogenesis, Gene families and clusters

SESSION-15: Regulation of gene activity in prokaryotes

Gere regulation in prokaryotes

SESSION-16: Regulation of gene activity in eukaryotes

Gene regulation in eukaryotes

SESSION-17: Molecular mechanism of mutation, repair and suppression -reverse mutations and suppressor mutations

Mutation, molecular mechanism,.........

SESSION-18: Bacterial plasmids, insertion (IS) and transposable (Tn) elements

SESSION-19: Molecular chaperones and gene expression

SESSION-20: RNA editing

SESSION-21: Gene isolation, synthesis and cloning

SESSION-22: Genomic and cDNA libraries, PCR based cloning, positional cloning

SESSION-23: Nucleic acid hybridization and immunochemical detection

SESSION-24: DNA sequencing

SESSION-25: DNA restriction and modification, ribozymes

SESSION-26: Gene silencing, Anti-sense RNA, 

SESSION-27: Micro RNAs (miRNAs)

SESSION-28: Genomics and proteomics

Genomics and Proteomics

SESSION-29: Metagenomics

SESSION-30: Transgenic bacteria and bioethics 

SESSION-31: Genetics of mitochondria and chloroplasts

SESSION-32: Concepts of Eugenics, Epigenetics, Genetic disorders.

Concept of epigenetics,..........

SESSION PLAN (PRACTICAL):

SESSION-1: Laboratory exercises in probability and chi-square

SESSION-2: Demonstration of genetic principles using laboratory

SESSION-3: Demonstration of genetic principles: Problems on monohybrid cross

SESSION-4: Demonstration of genetic principles: Problems on dihybrid cross

SESSION-5: Two-point test cross

SESSION-6: Chromosome mapping using three-point test cross, Tetrad analysis

SESSION-7: Extraction of total DNA by CTAB Method

SESSION-8: Quantification of DNA

SESSION-9: Electrophoresis - basic principles and running of amplified DNA

SESSION-10: PCR amplification

SESSION-11: Extraction of proteins (SDS-PAGE) and isozymes

SESSION-12: Use of Agrobacterium-mediated method

SESSION-13: Induction and detection of mutations through genetic tests

SESSION-14: Biolistic gun, practical demonstrations

SESSION-15: Detection of transgenes in the exposed plant material

SESSION-16: Visit to transgenic glasshouse and learning the practical considerations

QUESTION BANK:

MGPB0501 (2+1)_MCQ Type

ASSIGNMENTS

Assignment Topic List

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