CUTM2736 Forensic Serology and DNA Profiling

Teacher

Dr. Reena C. Jhamtani

Category

Core Courses

Course Attendees

Still no participant

Course Reviews

Still no reviews

CUTM2736 (4-0-0)

Course Objective :

1 To help students in examination of blood and other biological fluids.
2. To help students learn basics of cell biology and biomolecules.
3. To study the basic concept of DNA profiling and their analysis.

Course Outcomes:

On completion of this course, the successful students should be able to:

1 Understanding the principles and methodologies of forensic serology, including proficiency in techniques for the identification and analysis of bodily fluids such as blood, semen, saliva, and urine.
2 Comprehend the concepts related to cell biology and biochemistry of carbohydrates, lipids, proteins with their forensic significance.
3 Demonstrate Proficiency in techniques as isolation, extraction, and quantification of DNA to detect and analyze DNA profiles from forensic samples
4 Identify individuals based on DNA fingerprinting and understand the importance of mt-DNA in forensic investigation
5 Understand the legal and ethical considerations surrounding the use of DNA evidence in criminal investigations, and Apply critical thinking skills to evaluate forensic serology and DNA profiling protocols, for accuracy, reliability, and validity.

CO-PO Mapping :

S. NO DESCRIPTION MAPPING
CO-1 Understanding the principles and methodologies of forensic serology, including proficiency in techniques for the identification and analysis of bodily fluids such as blood, semen, saliva, and urine. PO1, PO2, PO3, PO4,  
CO-2 Comprehend the concepts related to cell biology and biochemistry of carbohydrates, lipids, proteins with their forensic significance. PO1, PO2
CO-3 Demonstrate Proficiency in techniques as isolation, extraction, and quantification of DNA to detect and analyze DNA profiles from forensic samples PO2, PO3, PO4, PO11
CO-4 Identify individuals based on DNA fingerprinting and understand the importance of mt-DNA in forensic investigation PO2, PO4, PO9
CO-5 Understand the legal and ethical considerations surrounding the use of DNA evidence in criminal investigations, and Apply critical thinking skills to evaluate forensic serology and DNA profiling protocols, for accuracy, reliability, and validity. PO5, PO6, PO7, PO9, PO11, PO12

SYLLABUS

Unit - 1

Basic Serology

  • Blood and its composition
  • Hemoglobin and its variants
  • Blood Typing/Grouping – ‘ABO’ system and its significance in forensic investigations.
  • Other blood group antigens - ‘Rh sub types’, MN, I, P, Kell, Duffy, Kidd, Lewis, Lutheran and Bombay blood group
  • Identification of other biological fluids like Saliva, Urine, Semen and Vaginal secretion, and their forensic

Unit - 2

Biochemistry and Cell Biology

  • Chemistry of Carbohydrates -Definition, classification and their importance in forensic investigation.
  • Chemistry of Lipids -Definition, classification and their importance in forensic investigation.
  • Types and properties of amino acids, structure of proteins and their importance in forensic investigation.
  • Introduction of plant and animal cells. Different eukaryotic cellular organelles, Plasma membrane, Transport across membrane Endoplasmic Reticulum, Golgi complex, Mitochondria, Chloroplast and Lysosomes. Organization of Nucleus and nuclear transport.,
  • Cell division-Mitosis and Meiosis;
  • Barr bodies and their importance in forensic investigation

Unit - 3

Basics of DNA extraction and Quantification

DNA extraction, quantification and separation:

  • Techniques and equipment for DNA extraction and purification,
  • Different methods of DNA quantitation,
  • DNA separation and detection techniques,
  • Polymerase Chain Reaction (PCR),

Unit-4

Introduction to forensic DNA analysis

  • History of DNA fingerprinting
  • DNA polymorphism, Genes and DNA markers in forensic DNA
  • Introduction to mitochondrial DNA and its forensic importance
  • Important case studies of DNA fingerprinting

SESSION PLAN

Session - 1

Blood and its components

Blood and its componenets

Session -2

Haemoglobin and its variants

Haemoglobin Structure

Session - 3

Blood Typing/Grouping – ‘ABO’ system and its significance in forensic

ABO Blood typing

Session - 4

Other blood group antigens - ‘Rh sub types’, MN, I, P, Kell, Duffy, Kidd, Lewis, Lutheran and Bombay blood group

ppp22625rapid

Session-5

Identification of other biological fluids like Saliva, Urine, Semen and Vaginal secretion, and their forensic significance.

Presumptive and confirmatory test for blood

Presumptive test for Blood

Session-6

Biological characteristics of saliva and its identification

Saliva (1)

Session-7

Composition of semen , vaginal secretion and its forensic importance

Different Tests for identification of Semen & Vaginal Secretion

Composition of body fluids (1)

SEMINAL STAIN

Session-8

Composition of Urine and its identification and Other biological fluids and its detection

Composition of body fluids (1)

Test for Urine

Session - 9

Biochemistry and Cell Biology: Chemistry of Carbohydrates -Definition, classification and their importance in forensic investigation.

Session - 10

Chemistry of Lipids -Definition, classification and their importance in forensic investigation.

Session - 11

Types and properties of amino acids, structure of proteins and their importance in forensic investigation.

Session - 12

Introduction of plant and animal cells.

Session - 13

Different eukaryotic cellular organelles, Plasma membrane, Transport across membrane Endoplasmic Reticulum, Golgi complex, Mitochondria, Chloroplast and Lysosomes.

Session - 14

Organization of Nucleus and nuclear transport.

Session - 15

Cell division-Mitosis and Meiosis

Session - 16

Barr bodies and their importance in forensic investigation

Session-17

Techniques for DNA extraction and purification

DNA Extraction_removed

Session-18

Different methods of DNA quantitation

DNAExtractionMethodsinForensicAnalysis2017

DNA quantification

Session-19

DNA separation and detection techniques

Session-20

Polymerase Chain Reaction (PCR)

Session-21

History of DNA fingerprinting

InvestGenet_2041-2223-4-22

Session - 22

DNA polymorphism

Session - 23

Genes and biomarkers in forensic DNA analysis

Genes in Forensic

Session - 24

Introduction to mitochondrial DNA and its forensic importance

MitochondrialDNA

Chapter-16ApplicationsofMitochondrialDNA

Session - 25

Important case studies of DNA Fingerprinting

Our Main Teachers

Associate Professor at the School of Forensic Sciences, Centurion University of Technology and Management, Orrisa, Bhubaneshwar.