CUTM2516 Forensic Serology and DNA Profiling

Teacher

TAMOSA MUKHERJEE

Category

Core Courses

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CUTM2516 (4-1-0)

Course Objective :

To acquaint the students about different body fluids and their analysis. Impart the knowledge of DNA profiling and individualisation.

Course Outcome :

  • Acquire skills to identify, characterise and analyse the various body fluids using different serological techniques found in a crime scene.
  • Demonstrate the different conventional and modern techniques for DNA isolation, extraction, quantitation and purification of DNA. Understand the basic concept of individualization and uniqueness of DNA in identification of individuals.
  • Identify individuals based on DNA Profiling technique.
  • Understand the significance of microbiology in forensic science and how it can be involved in evidence analysis.

Syllabus

Unit - 1

Basic Serology

  • Blood and its
  • Hemoglobin and its
  • Blood Typing/Grouping – ‘ABO’ system and its significance in forensic
  • Other blood group antigens - ‘Rh sub types’, MN, I, P, Kell, Duffy, Kidd, Lewis, Lutheran and Bombay blood group
  • Presumptive and confirmatory test for blood

Unit - 2

Identification of Saliva and Other Biological Fluids

  • Biological characteristics of saliva and its identification
  • Composition of semen , vaginal secretion and its forensic importance
  • Different Tests for identification of Semen & Vaginal Secretion
  • Composition of Urine and its identification
  • Other biological fluids and its detection

Unit - 3

Basics of DNA extraction and Quantification

  • Conventional techniques for DNA isolation
  • Modern techniques and equipment for DNA extraction and purification
  • Different methods of DNA quantitation
  • DNA separation techniques

Unit-4

Introduction to forensic DNA analysis

  • History of DNA fingerprinting
  • DNA polymorphism, Genes and DNA markers in forensic DNA
  • Introduction to mitochondrial DNA and its forensic importance
  • Important case studies of DNA fingerprinting

Unit-5

Forensic Microbiology

 

  • Scope of microbiology in forensic investigation
  • Sterilization techniques - Physical agents: Dry heat, wet heat and cold sterilization, filtration, radiation; Chemical agents (Disinfectants, antibiotics, alcohols) and their
  • Microbial diversity and its application in forensic
  • Different methods for isolation of microorganisms from forensic samples like vomit, stool, stomach wash and residual food.

Session - 1

Blood and its components

Blood and its componenets

Session -2

Haemoglobin and its variants

Haemoglobin Structure

Session - 3

Blood Typing/Grouping – ‘ABO’ system and its significance in forensic

ABO Blood typing

Session - 4

Other blood group antigens - ‘Rh sub types’, MN, I, P, Kell, Duffy, Kidd, Lewis, Lutheran and Bombay blood group

ppp22625rapid

Session-5

Presumptive and confirmatory test for blood

Presumptive test for Blood

 

Session-6

Biological characteristics of saliva and its identification

Saliva (1)

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Session-7

Composition of semen , vaginal secretion and its forensic importance

Different Tests for identification of Semen & Vaginal Secretion

Composition of body fluids (1)

SEMINAL STAIN

Session-8

Composition of Urine and its identification and Other biological fluids and its detection

Composition of body fluids (1)

Test for Urine

Session - 9

Conventional techniques for DNA isolation

Conventional DNA Extraction Methods

Session-10

Modern techniques and equipment for DNA extraction and purification

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Session-11

Different methods of DNA quantitation

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DNA quantification

Session-12

History of DNA fingerprinting

InvestGenet_2041-2223-4-22

Session - 13

DNA polymorphism, Genes and DNA markers in forensic DNA

Genes in Forensic

Session - 14

Introduction to mitochondrial DNA and its forensic importance

MitochondrialDNA

Chapter-16ApplicationsofMitochondrialDNA

Session - 15

Scope of microbiology in forensic investigation

Session - 16

Sterilization techniques - Physical agents: Dry heat, wet heat and cold sterilization, filtration, radiation; Chemical agents (Disinfectants, antibiotics, alcohols) and their mechanisms

Methods_of_Sterilization_and_Disinfection-1 (1)

Session - 17

Microbial diversity and its application in forensic

Session-18

Different methods for isolation of microorganisms from forensic samples like vomit, stool, stomach wash and residual food.

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