CUTM2516 Forensic Serology and DNA Profiling

Teacher

TAMOSA MUKHERJEE

Category

Core Courses

Course Attendees

Still no participant

Course Reviews

Still no reviews

CUTM2516 (4-1-0)

Course Objective :

To acquaint the students about different body fluids and their analysis.

Impart the knowledge of DNA profiling and individualisation.

Course Outcome :

On completion of this course, the successful students should be able to:

  1. Acquire skills to identify, characterise and analyse the various body fluids using different serological techniques found in a crime scene.
  2. Demonstrate the different conventional and modern techniques for DNA isolation, extraction, quantitation and purification of DNA. Understand the basic concept of individualization and uniqueness of DNA in identification of individuals.
  3. Identify individuals based on DNA Profiling technique.
  4. Understand the significance of microbiology in forensic science and how it can be involved in evidence analysis.

CO-PO Mapping :

SNO DESCRIPTION POMAPPING
CO1 The importance of biological fluids – blood, urine, semen, saliva, sweat  – in crime investigations. PO1, PO2, PO5, PO6
CO2 Know about the usefulness of genetic markers in forensic investigations. PO1, PO2, PO5, PO6, PO11, PO12
CO3 Understand the basic principle of DNA analysis and it’s significance PO1,PO2, PO5, PO6, PO11, PO12

Syllabus

Unit - 1

Basic Serology

  • Blood and its
  • Hemoglobin and its
  • Blood Typing/Grouping – ‘ABO’ system and its significance in forensic
  • Other blood group antigens - ‘Rh sub types’, MN, I, P, Kell, Duffy, Kidd, Lewis, Lutheran and Bombay blood group
  • Presumptive and confirmatory test for blood

Unit - 2

Identification of Saliva and Other Biological Fluids

  • Biological characteristics of saliva and its identification
  • Composition of semen , vaginal secretion and its forensic importance
  • Different Tests for identification of Semen & Vaginal Secretion
  • Composition of Urine and its identification
  • Other biological fluids and its detection

Unit - 3

Basics of DNA extraction and Quantification

  • Conventional techniques for DNA isolation
  • Modern techniques and equipment for DNA extraction and purification
  • Different methods of DNA quantitation
  • DNA separation techniques

Unit-4

Introduction to forensic DNA analysis

  • History of DNA fingerprinting
  • DNA polymorphism, Genes and DNA markers in forensic DNA
  • Introduction to mitochondrial DNA and its forensic importance
  • Important case studies of DNA fingerprinting

Unit-5

Forensic Microbiology

 

  • Scope of microbiology in forensic investigation
  • Sterilization techniques - Physical agents: Dry heat, wet heat and cold sterilization, filtration, radiation; Chemical agents (Disinfectants, antibiotics, alcohols) and their
  • Microbial diversity and its application in forensic
  • Different methods for isolation of microorganisms from forensic samples like vomit, stool, stomach wash and residual food.

Session - 1

Blood and its components

Blood and its componenets

Session -2

Haemoglobin and its variants

Haemoglobin Structure

Session - 3

Blood Typing/Grouping – ‘ABO’ system and its significance in forensic

ABO Blood typing

Session - 4

Other blood group antigens - ‘Rh sub types’, MN, I, P, Kell, Duffy, Kidd, Lewis, Lutheran and Bombay blood group

ppp22625rapid

Session-5

Presumptive and confirmatory test for blood

Presumptive test for Blood

 

Session-6

Biological characteristics of saliva and its identification

Saliva (1)

Session-7

Composition of semen , vaginal secretion and its forensic importance

Different Tests for identification of Semen & Vaginal Secretion

Composition of body fluids (1)

SEMINAL STAIN

Session-8

Composition of Urine and its identification and Other biological fluids and its detection

Composition of body fluids (1)

Test for Urine

Session - 9

Conventional techniques for DNA isolation

Conventional DNA Extraction Methods

Session-10

Modern techniques and equipment for DNA extraction and purification

DNA Extraction_removed

Session-11

Different methods of DNA quantitation

DNAExtractionMethodsinForensicAnalysis2017

DNA quantification

Session-12

History of DNA fingerprinting

InvestGenet_2041-2223-4-22

Session - 13

DNA polymorphism, Genes and DNA markers in forensic DNA

Genes in Forensic

Session - 14

Introduction to mitochondrial DNA and its forensic importance

MitochondrialDNA

Chapter-16ApplicationsofMitochondrialDNA

Session - 15

Scope of microbiology in forensic investigation

Session - 16

Sterilization techniques - Physical agents: Dry heat, wet heat and cold sterilization, filtration, radiation; Chemical agents (Disinfectants, antibiotics, alcohols) and their mechanisms

Methods_of_Sterilization_and_Disinfection-1 (1)

Session - 17

Microbial diversity and its application in forensic

Session-18

Different methods for isolation of microorganisms from forensic samples like vomit, stool, stomach wash and residual food.

Our Main Teachers