INSTRUMENTAL METHODS OF ANALYSIS(THEORY)

Teacher

Mr. HARA GOURI MISHRA

Category

Core Courses

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Course Name : INSTRUMENTAL METHODS OF ANALYSIS

Code(Credit): BPHT4101(4-0-0)

Course Objectives

  • This subject deals with the application of instrumental methods in qualitative and quantitative analysis of drugs.
  • This subject is designed to impart fundamental knowledge of the principles and instrumentation of spectroscopic and chromatographic techniques.
  • This also emphasizes theoretical and practical knowledge of modern analytical instruments that are used for drug testing.

Learning Outcomes

  • Understand the interaction of matter with electromagnetic radiations and its applications in drug analysis.
  • Understand the chromatographic separation and analysis of drugs.
  • Perform quantitative & qualitative analysis of drugs using various analytical instruments.

Course Syllabus

MODULE –I

UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, the solvent effect on absorption spectra, Beer and Lambert’s law, Derivation, and deviations.

Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photovoltaic cell, Silicon Photodiode.

Applications - Spectrophotometric titrations, Single-component and multi-component analysis

Fluorimetry

Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation, and applications

MODULE –II

IR spectroscopy

Introduction, fundamental modes of vibrations in polyatomic molecules, sample handling, factors affecting vibrations

Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications

Flame Photometry

Principle, interferences, instrumentation and applications

Atomic absorption spectroscopy

Principle, interferences, instrumentation, and applications

Nepheloturbidometry-

Principle, instrumentation, and applications

MODULE –III

Introduction to chromatography

Adsorption and partition column chromatography-

Methodology, advantages,disadvantages and applications.

Thin-layer chromatography-

Introduction, Principle, Methodology, Rf values, advantages, disadvantages, and applications.

Paper chromatography-

Introduction, methodology, development techniques, advantages, disadvantages, and applications

Electrophoresis

Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications

 

MODULE-IV

Gas chromatography -

Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications

High-performance liquid chromatography (HPLC)-

Introduction, theory, instrumentation, advantages and applications.

MODULE –V

Ion exchange chromatography-

Introduction, classification, ion exchange resins, properties, mechanism of the ion exchange process, factors affecting ion exchange, methodology and applications

Gel chromatography-

Introduction, theory, instrumentation, and applications

Affinity chromatography-

Introduction, theory, instrumentation, and applications

REFERENCE BOOKS-

  1. Instrumental Methods of Chemical Analysis by B.K.Sharma
  2. Organic Spectroscopy by Y.R. Sharma
  3. Text book of Pharmaceutical Analysis by Kenneth A. Connors
  4. Vogel's Text book of Quantitative Chemical Analysis by A.I.Vogel
  5. Organic Spectroscopy by William Kemp
  6. Spectrophotometric identification of Organic Compounds by Silverstein

Session Plan

Session 1

UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts

Pdf:    ELECTRON TRANSITIONS,AUXOCHROMES

VIDEO: https://www.youtube.com/watch?v=dX_AJXPItAQ&t=92s

Session 2

UV Visible spectroscopy

solvent effect on absorption spectra,

Beer and Lambert’s law, Derivation and deviations

PDF: Solvent effect on absorption spectra B&L LAW

Session 3

UV Visible spectroscopy

Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photovoltaic cell, Silicon Photodiode.photodetectors INSTRUMENTATION OF UV

Session 4

UV Visible spectroscopy

Applications - Spectrophotometric titrations,

Single-component, and multi-component analysis

PDF: applicationofu-v-spectroscopy-120416145659-phpapp02

Session 5

FLUORIMETRY
Introduction and Theory

PDF:apaseminarppt-150224044240-conversion-gate01

Session 6

Fluorimetry

Concepts of singlet, doublet and triplet electronic states

PDF:14.7_ Fluorescence SINGLET DOUBLET TRIPLET STATE

Session 7

Fluorimetry

Internal and external conversions,

Factors affecting fluorescence

PDF: fifactors-150223045100-conversion-gate02

Session 8

Fluorimetry

Quenching

Instrumentation of Fluorimetry

PDF: presentationfinal-161009160828

flourimetry-140618015916-phpapp01

Session 9

Fluorimetry

Applications of Fluorimetry

PDF: APPLICATIONS OF FLUORIMETRY

Session 10

IR spectroscopy
Introduction,

Fundamental modes of vibrations in polyatomic molecules

PDF: https://www.slideshare.net/chiranjibi68/theory-of-ir-spectroscopy

Session 11

IR spectroscopy
Sample handling in IR spectroscopy,

Factors affecting vibrations

PDF: https://www.slideshare.net/yousufzaidi/4424-structure-determination-through-ir

Session 12

IR spectroscopy

Instrumentation - Sources of radiation, wavelength selectors,

PDF:INSTRUMENTATION OF IR spectroscopy

Session 13

IR spectroscopy

Detectors - Golay cell, Bolometer

PDF:BOLOMETER GOLAY CELL

Session 14

IR spectroscopy

Detectors

Thermocouple, Thermister, Pyroelectric detector

PDF:Thermocouple, Thermister, Pyroelectric detector

Session 15

IR spectroscopy

Applications of IR spectroscopy

PDF APPLICATIONS OF IR SPECTROSCOPY

Session 16

Flame Photometry-

Principle, Interferences

PDF: FLAME PHOTOMETRY

Session 17

Flame Photometry-

Instrumentation of Flame Photometry

Applications of Flame Photometry

PDF:APPLICATIONS OF FLAME PHOTOMETRY

Session 18

Atomic absorption spectroscopy

Principle, interferences

PDF:Atomic absorption spectrometry

Session 19

Atomic absorption spectroscopy

Instrumentation of Atomic absorption spectroscopy

Applications of Atomic absorption spectroscopy

PDF:Instrumentation of Atomic Absorption Spectroscopy

Applications of Atomic Absorption Spectroscopy

Session 20

Nepheloturbidometry

Principle, instrumentation and applications

PDF:Nepheloturbidometry

Session 21

Introduction to chromatography

PDF:Chromatography

Session 22

Adsorption and partition column chromatography-

Methodology

PDF:Adsorption Chromatography METHODOLOGY

Partition Chromatography METHODOLOGY

Session 23

Adsorption and partition column chromatography-

Advantages, disadvantages and applications

PDF:Partition Chromatography Applications

Session 24

Thin-layer chromatography-

Introduction, Principle, Methodology

https://www.youtube.com/watch?v=rMGQavOMAmc

Session 25

Thin-layer chromatography-

Rf values, advantages, disadvantages and applications

PDF: Thin Layer Chromatography Applications

 

Session 26

Paper chromatography-

Introduction, methodology

Session 27

Paper chromatography-

Development techniques, advantages, disadvantages and applications

Session 28

Electrophoresis

Introduction

Session 29

Electrophoresis

Factors affecting electrophoretic mobility

Techniques of paper Electrophoresis

Session 30

Electrophoresis

Techniques of gel electrophoresis,

Techniques of capillary electrophoresis

Applications of electrophoresis

Session 31

Gas chromatography

Introduction, theory

Session 32

Gas chromatography

instrumentation, derivatization, temperature programming

Session 33

Gas chromatography

Advantages, disadvantages and applications

Session 34

High-performance liquid chromatography (HPLC)

Introduction

Session 35

High-performance liquid chromatography (HPLC)

Theory

Session 36

High-performance liquid chromatography (HPLC)

Introduction

Session 37

High-performance liquid chromatography (HPLC)

Instrumentation

Session 38

High-performance liquid chromatography (HPLC)

Advantages of HPLC

Applications ofHPLC

Session 39

Ion exchange chromatography

Introduction, classification

Session 40

Ion exchange chromatography

Ion exchange resins, properties,

Mechanism of the ion exchange process

Session 41

Ion exchange chromatography

Factors affecting ion exchange,

methodology and

Applications of Ion exchange chromatography

Session 42

Gel chromatography

Introduction, theory

Session 43

Gel chromatography

Instrumentation of Gel chromatography

Applications of Gel chromatography

Session 44

Affinity chromatography

Introduction, theory

Session 45

Affinity chromatography

Instrumentation of Affinity chromatography

Applications of Affinity chromatography

Case Studies

Case Studies

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