FUNDAMENTALS OF PLANT BIOCHEMISTRY

Teacher

Dr. Raghu Gogada

Category

Core Courses

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Course Name :FUNDAMENTALS OF PLANT BIOCHEMISTRY

Code(Credit) : ASBC2101:3(2+1)

Course Objectives:

Understand the biochemistry plant defence mechanism, Identify the toxic compounds in plants, Describe the kinetics and characterisation of enzymes, Identify the detoxification mechanisms. To provide education that leads to comprehensive understanding of the principles and practices of biochemistry.

Learning Outcomes:

In this course, students will extend their knowledge of biochemistry fundamentals and will learn about the significance of biochemistry and important metabolic processes taking place in plants. Acquire a detailed knowledge about the chemistry of carbohydrates, lipids, proteins and amino acids and their classification, structural organization of proteins, metabolism of saccharides, lipids. The students get acquainted with the widely used techniques in plant biochemistry like qualitative and quantitative tests for the important biomolecules.

Course Syllabus:

Theory:
Importance of Biochemistry. Properties of Water, pH and Buffer. Carbohydrate: Importance and classification. Structures of Monosaccharides, Reducing and oxidizing properties of Monosaccharides, Mutarotation; Structure of Disaccharides and Poly saccharides. Lipid: Importance and classification; Structures and properties of fatty acids; storage lipids and membrane lipids. Proteins: Importance of proteins and classification; Structures, titration and zwitterions nature of amino acids; Structural organization of proteins. Enzymes: General properties; Classification; Mechanism of action; Michaelis & Menten and Line Weaver Burk equation & plots; Introduction to allosteric enzymes. Nucleic acids: Importance and classification; Structure of Nucleotides, A, B & Z DNA; RNA: Types and Secondary & Tertiary structure. Metabolism of carbohydrates: Glycolysis, TCA cycle, Glyoxylate cycle, Electron transport chain. Metabolism of lipids: Beta oxidation, Biosynthesis of fatty acids.

Practical:
Preparation of solution, pH & buffers, Qualitative tests of carbohydrates and amino acids. Quantitative estimation of glucose/ proteins. Titration methods for estimation of amino acids/lipids, Effect of pH, temperature and substrate concentration on enzyme action, Paper chromatography/ TLC demonstration for separation of amino acids/ Monosaccharides. Sterilization techniques.

References:

1. Satyanarayana U,Textbook of biochemistry,2007, 3rd edition, Books and Allied (P) ltd, Kolkata.
2. Lehninger, Albert L., David L. Nelson, and Michael M. Cox. 2000. Lehninger principles of biochemistry. New York: Worth Publishers.
3. D T Plummer, An Introduction to Practical Biochemistry, 1987, 3rd edition,McGraw-Hill, USA.
4. S. Sadasivam and A. Manickam, “Biochemical Methods,” New Age International (P) Limited, New Delhi, Vol. 2. 1996, pp. 124-126.

Session Plan

Session 1

Importance of Biochemistry

1_Importance of Biochemistry

See my story: Introduction to Biochemistry

https://spark.adobe.com/video/wzjDHZ8NyNMIp

https://www.youtube.com/watch?v=NoyM9zQamE0

https://www.youtube.com/watch?v=CHJsaq2lNjU

Session 2

Properties of water, buffer and pH

2_water, buffer,pH

https://www.youtube.com/watch?v=i1LyWQ_lPik

https://www.youtube.com/watch?v=nNQKI9CdjAA

Session 3

Carbohydrate: Importance
and classification.

3_Carbohydrate

https://www.youtube.com/watch?v=wFYsufJ9XMM
https://www.youtube.com/watch?v=LeOUIXbFyqk

Session 4

Lipids: Importance and classification; Structures and properties of fatty acids; storage lipids and membrane<br

4_Lipids

https://www.youtube.com/watch?v=-3TmDPzyBeY

https://www.youtube.com/watch?v=8FLT5fKUWks

Session 5

Proteins: Importance of proteins and classification; Structures, titration and zwitterions
nature of amino acids; Structural organization of proteins.

5_Proteins

https://www.youtube.com/watch?v=3vy__KvTi6I&list=PL9jo2wQj1WCNTJhgaXpsI1tzi0bemyl4j

Session 6

Enzymes: General properties;
Classification; Mechanism of action; Michaelis & Menten and Line Weaver Burk equation & plots; Introduction to allosteric enzymes.

6_Enzymes

https://www.youtube.com/watch?v=CI6zVmlQipU

https://www.youtube.com/watch?v=M9u3uf4KRkQ&list=PLb0WW0k29aHpnv9LiIP5cV0RbqssLiNRk

Session 7

Nucleic acids: Importance and classification; Structure of Nucleotides, A, B & Z DNA; RNA: Types and Secondary & Tertiary structure.

7_Nuclic acid

https://www.youtube.com/watch?v=1Wc4jTH2v_w&list=PL9jo2wQj1WCNG9mFuNBmJ1m7x1skBNKw-

https://www.youtube.com/watch?v=0lZRAShqft0

Session 8

Metabolism of carbohydrates: Glycolysis, TCA cycle, Glyoxylate cycle, Electron transport chain.

8-Metabolism of Carbohydrate

https://www.youtube.com/watch?v=BdhSoINFFxE

https://www.youtube.com/watch?v=HrYRMknYHrk

Session 9

Metabolism of lipids: Beta oxidation, Biosynthesis of fatty acids.

9-Metabolism of Lipid

https://www.youtube.com/watch?v=Tf9jN4zw9EY&list=PLRpz4J5dUMCf0Jk8oojz__5TuaXtPNLmX

https://www.youtube.com/watch?v=__jS-pjzb5k

https://www.youtube.com/watch?v=fImrTylULx8

Session 10

Practice-1

Preparation of Buffer Solutions https://www.youtube.com/watch?v=aOUSywHjoI4

methodsforbufferpreparation

Session 11

Practice-2</

Session 13

Practice-4
Qualitative analysis of Proteins https://www.youtube.com/watch?v=OsdhNtNNNds

Biuret’s Test https://www.youtube.com/watch?v=bi2LtNh5h50

Ninhydrin Test https://www.youtube.com/watch?v=6Yl62icAKsQ

Session 14

Practice-5

Estimation of Protein by Lowry’s Method https://labmonk.com/estimation-of-protein-by-folin-lowry-method

Session 15

Practice-6

Effect of pH on enzyme (catalase) https://www.youtube.com/watch?v=_DZtsp3-7IU
Effect of substrate concentration on enzyme https://www.youtube.com/watch?v=UQww6s-64wo

Session 16

Practice-7
Separation of Plant Pigments by TLC and calculation Rf values https://www.youtube.com/watch?v=6sZBtANyuZ8

Session 17

Practice-8

Session 18

Practice-9
Chemical Sterilization Techniques https://study.com/academy/lesson/chemical-sterilization-methods.html and https://www.thermofisher.com/in/en/home/references/gibco-cell-culture-basics/aseptic-technique.html

Our Main Teachers

Dr. Raghu Gogada

Associate Professor and Head, Department of Biochemistry and Plant Physiology, MSSSoA, CUTM
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Dr. Raghu Gogada had completed his B.Sc. in Biochemistry (2001) from Silver Jubilee Govt. Degree College, Kurnool, SKD University, Anantapur and M.Sc. in EnvironmentalBiotechnology (2004) from Jawaharlal Nehru Technological University (JNTU), and pursued his dissertation from Center for Cellular and Molecular Biology (CCMB), Hyderabad, INDIA and study focused on “Investigations on the biochemical and genetic […]