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Understand the biochemistry plant defence mechanism, Identify the toxic compounds in plants, Describe the kinetics and characterisation of enzymes, Identify the detoxification mechanisms. To provide education that leads to comprehensive understanding of the principles and practices of biochemistry.
In this course, students will extend their knowledge of biochemistry fundamentals and will learn about the significance of biochemistry and important metabolic processes taking place in plants. Acquire a detailed knowledge about the chemistry of carbohydrates, lipids, proteins and amino acids and their classification, structural organization of proteins, metabolism of saccharides, lipids. The students get acquainted with the widely used techniques in plant biochemistry like qualitative and quantitative tests for the important biomolecules.
Importance of Biochemistry. Properties of Water, pH and Buffer. Carbohydrate: Importance and classification. Structures of Monosaccharides, Reducing and oxidizing properties of Monosaccharides, Mutarotation; Structure of Disaccharides and Poly saccharides. Lipid: Importance and classification; Structures and properties of fatty acids; storage lipids and membrane lipids. Proteins: Importance of proteins and classification; Structures, titration and zwitterions nature of amino acids; Structural organization of proteins. Enzymes: General properties; Classification; Mechanism of action; Michaelis & Menten and Line Weaver Burk equation & plots; Introduction to allosteric enzymes. Nucleic acids: Importance and classification; Structure of Nucleotides, A, B & Z DNA; RNA: Types and Secondary & Tertiary structure. Metabolism of carbohydrates: Glycolysis, TCA cycle, Glyoxylate cycle, Electron transport chain. Metabolism of lipids: Beta oxidation, Biosynthesis of fatty acids.
Preparation of solution, pH & buffers, Qualitative tests of carbohydrates and amino acids. Quantitative estimation of glucose/ proteins. Titration methods for estimation of amino acids/lipids, Effect of pH, temperature and substrate concentration on enzyme action, Paper chromatography/ TLC demonstration for separation of amino acids/ Monosaccharides. Sterilization techniques.
1. Satyanarayana U,Textbook of biochemistry,2007, 3rd edition, Books and Allied (P) ltd, Kolkata.
2. Lehninger, Albert L., David L. Nelson, and Michael M. Cox. 2000. Lehninger principles of biochemistry. New York: Worth Publishers.
3. D T Plummer, An Introduction to Practical Biochemistry, 1987, 3rd edition,McGraw-Hill, USA.
4. S. Sadasivam and A. Manickam, “Biochemical Methods,” New Age International (P) Limited, New Delhi, Vol. 2. 1996, pp. 124-126.
Importance of Biochemistry
See my story: Introduction to Biochemistry
Lipids: Importance and classification; Structures and properties of fatty acids; storage lipids and membrane<br
Proteins: Importance of proteins and classification; Structures, titration and zwitterions
nature of amino acids; Structural organization of proteins.
Enzymes: General properties;
Classification; Mechanism of action; Michaelis & Menten and Line Weaver Burk equation & plots; Introduction to allosteric enzymes.
Nucleic acids: Importance and classification; Structure of Nucleotides, A, B & Z DNA; RNA: Types and Secondary & Tertiary structure.
Metabolism of carbohydrates: Glycolysis, TCA cycle, Glyoxylate cycle, Electron transport chain.
Metabolism of lipids: Beta oxidation, Biosynthesis of fatty acids.
2. Bradfoed’s Test https://www.youtube.com/watch?v=yQfMqvOxPrc
3. Molisch’s Test https://www.youtube.com/watch?v=etE8LCWvb-U
4. Benedict’s Test https://www.youtube.com/watch?v=TDFbtEwbmz0
5. Seliwanoff’s Test https://www.youtube.com/watch?v=88hWF1-RrTQ
Qualitative analysis of Proteins https://www.youtube.com/watch?v=OsdhNtNNNds
Biuret’s Test https://www.youtube.com/watch?v=bi2LtNh5h50
Ninhydrin Test https://www.youtube.com/watch?v=6Yl62icAKsQ
Chemical Sterilization Techniques https://study.com/academy/lesson/chemical-sterilization-methods.html and https://www.thermofisher.com/in/en/home/references/gibco-cell-culture-basics/aseptic-technique.html
Dr. Raghu Gogada had completed his B.Sc. in Biochemistry (2001) from Silver Jubilee Govt. Degree College, Kurnool, SKD University, Anantapur and M.Sc. in EnvironmentalBiotechnology (2004) from Jawaharlal Nehru Technological University (JNTU), and pursued his dissertation from Center for Cellular and Molecular Biology (CCMB), Hyderabad, INDIA and study focused on “Investigations on the biochemical and genetic […]