Sow your seed in the morning, At evening let not your hands be idle

per person /

Free

Home Courses

Seed Production Using Manual and Molecular Methods

Seed Production Using Manual and Molecular Methods

Teacher

Dr. Aninda Chakraborty

Category

Domain Courses

Course Attendees

Still no participant

Course Reviews

Still no reviews


Domain Track Title : Seed Production Using Manual and Molecular Methods

Credits (T-P-P): 3-15-11 (29 credits)

Domain Track Objectives:

  • To acquaint students with conventional and modern breeding methods and its viable application in varietal development.
  • To impart a comprehensive knowledge of seed production in vegetable and cereal crops with adequate practical training.
  • To apprise students with the legislative provisions and processes as well as the mechanisms of quality control and seed certification.

CO-PO Mapping:

 

COs Course outcomes Mapping COs with POs
CO1 At the conclusion of this domain, students will have expanded their experiential learning, practical knowledge, and hands-on training in the following areas: hybrid seed development using the emasculation and pollination technique; application of molecular markers in seed production; physical and genetic purity testing for high-quality seed production; and the certification process for seeds.
Variety and hybrid seed production for vegetable and cereal crops; physical and genetic purity testing for high-quality seed production; several techniques for extracting seeds; and the certification process for seeds.
PO1
CO2 Launch a seed production initiative to meet the market's demand for high-quality seed and boost revenue. PO2, PO3
CO3 Marketing and distribution hybrid seed in the market so that crops can be raised sustainably. PO4

PO1: Knowledge Enhancement

PO2: Professional Enhancement

PO3: Entrepreneurship Skills

PO4: Communication & Extension Skill

Courses Division:

  1. Breeding methods: conventional and molecular approach (1-2-0)

  2. Seed production of vegetable and cereal crops (1-2-0)

  3. Seed certification (1-2-0)

  4. Hybridization techniques (0-3-0)

  5. Vegetable Seed production (0-3-0)

  6. Cultivar purity and seed quality testing (0-3-0)

  7. Practices in Units/Field/Lab/Project (0-0-11)

Domain Syllabus:

1. Breeding methods: conventional and molecular approach (1-2-0)

Theory

 Classification of Plant Breeding methods,Genetic basis and breeding methods in self- pollinated crops, Hybridization techniques and handling of segregating population, Genetic basis and breeding methods of cross-pollinated crops,Heterosis and inbreeding depression,Hybrid breeding- steps /operations in production of hybrid varieties,Male sterility and its application in hybrid seed production, Breeding methods in asexually propagated crops, Special breeding techniques: Mutation breeding,Special breeding techniques: Breeding for abiotic stresses,Special breeding techniques: Breeding for biotic stresses,DNA markers and its application in plant breeding,Marker assisted selection (MAS) ,Quantitative trait analysis(QTL),Cultivar development-variety testing procedures, release and notification system in India ,IPR

Practical

 Emasculation and hybridization techniques in Rice,Emasculation and hybridization techniques in maize, Emasculation and hybridization techniques in vegetable crops: Tomato,Emasculation and hybridization techniques in vegetable crops: Okra,Emasculation and hybridization techniques in vegetable crops: Brinjal,Handing of segregating populations,Designs used in plant breeding experiment: RBD analysis,Estimation of heterosis ,Inbreeding depression ,Heritability,Learning techniques in hybrid seed production using male-sterility in field crops, Use of male sterility in hybrid seed production: Rice, Genetic Mechanisms for Hybrid Seed Production in vegetable crops,Visit to AICRP plots to know about different trials for cultivar development,DNA isolation, DNA purity ,DNA quantification tests, DNA finger printing using PCR-based DNA markers,Gel electrophoresis, Gel scoring ,Data analysis for tagging,Phylogenetic relationship,Use of standard MAS procedures- screening crops for resistant to different biotic stress traits,Use of standard MAS procedures- screening crops for resistant to different abiotic stress traits, Breeding for quality traits,Breeding for drought tolerant using MAS,Breeding for salinity tolerant using MAS,Breeding for heat tolerant using MAS,Breeding for cold tolerant using MAS,Breeding for mineral toxicity using MAS,Release of new variety,Variety development through mutation breeding

Suggested Readings

  1. Allard, R.W.1960. Principles of Plant Breeding. John Wiley & Sons, New York
  2. Caetano-Anolles G & Gresshoff PM. 1998. DNA Markers: Protocols, Applications and Overviews. Wiley-VCH.
  3. Chopra VL. 2001. Breeding Field Crops. Oxford & IBH.
  4. Kalloo, G.1994, Vegetable Breeding, Panima Educational Book Agency, New Delhi
  5. Singh,P. 2006, Essential of Plant Breeding. Kalyani Publishers, Ludhiana
  6. Singh B. D. 2006. Plant Breeding. Kalyani Publishers.

2. Seed production of vegetable and cereals crops (1-2-0)

Theory

Importance and scope of vegetable seed production in India; Planning for certified, foundation and breeder seed production; Seed quality: Certification standards for self and cross-pollinated and vegetatively propagated crops; Principles of seed production in vegetable crops; Seed production in varieties and hybrids of cereals crops: paddy and maize; Seed production in varieties and hybrids of vegetable crops: tomato, brinjal and okra; Harvesting/picking stage and seed extraction in vegetables; Seed drying, seed processing and seed storage; Seed health and measures for pest and disease control during storage; Seed Invigouration; Seed Village

Practical

Selection of suitable areas/locations, seed/planting material for quality seed production; Determination of planting ratios for hybrid seed production of cereals; Study of floral biology of Paddy; Study of floral biology of Maize; Study of floral biology of Tomato; Study of floral biology of Okra; Study of floral biology of Eggplant; Determination of planting ratios for hybrid seed production of vegetables; Seed extraction methods and their effect on quality of vegetables; Seed and seedling vigour test; Practice on seed production of varieties and hybrids in cereal crops; Practice on seed production of varieties and hybrids in vegetable crops; Visit to seed production farms; Visit to seed testing laboratories; Visit to seed processing unit.

Reference

  1. Agarwal R.L. 1997. Seed Technology. 2nd Ed. Oxford & IBH.
  2. Desai BB, Katecha, PM & Salunke DK.1997. Seed Hand Book: Biology, Production, Processing and Storage. Marcel Dekker.
  3. Desai BB. 2004. Seeds Handbook. Marcel Dekker.
  4. George RAT. 1980. Vegetable Seed Technology. A Technical Guide to Vegetable Seed Production, Processing, Storage and Quality Control. FAO, Rome

3. Seed certification (1-2-0)

Theory

Seed policy; Seed Certification; Seed Act; Seed Control Order; Indian Minimum Seed Certification Standards (I.M.S.C.S.); Phases, Planning and management of seed certification programmes; Field Inspection: principles, phases and procedures, post-harvest inspection and evaluation; Seed sampling; Seed testing; Seed Viability; Varietal Identification; Grant of certificate and certification fee and other service charges; Seed marketing: structure and organization and pricing policy; Role of WTO and OECD in seed marketing; IPR and PPV&FRA

Practical

General procedure of seed certification; Identification of weed and other crop seeds as per specific crops; Field inspection at different stages of a crop and observations recorded; Inspection and sampling at harvesting/threshing for seed law enforcement; Inspection and sampling at processing and after processing for seed law enforcement; Sampling methods and seed quality test; Seed viability testing; Seed germination; Specifications for tags and labels to be used for certification purpose; Varietal Identification through Grow Out Test; Varietal Identification through Electrophoresis; Molecular test for pre- and post-harvest quality control; Biochemical test for pre- and post-harvest quality control; Visits to Seed Testing Laboratory; Visit to private and public sector seed companies; Visit to certification agency.

Reference

  1. Agarwal R.L. 1997. Seed Technology. Oxford & IBH.
  2. Legislation on Seeds, 1992. NSC Ltd., Department of Agriculture and Cooperation, Ministry of Agriculture, New Delhi.
  3. Nema NP. 1986. Principles of Seed Certification and Testing. Allied Publishers.
  4. Tunwar NS & Singh SN. 1988. Indian Minimum Seed Certification Standards. CSCB, Ministry of Agriculture, New Delhi.

4. Hybridization techniques (0-3-0)

Practice

Growing different genotypes of a crop using appropriate experimental design; Practicing emasculation and hybridization techniques in various crops; Harvesting of F1 seeds; DNA isolation of F1 progenies; Check the hybrid (F1) fidelity using molecular markers; Visit to research station to learn techniques in hybrid seed production using male sterility in field crops; Visit to hybrid seed production plots; Visit to research station to understand MAS procedures

5. Seed production of vegetable crops (0-3-0)

Practice

Selection of suitable areas/locations for high quality seed/planting material production;  Learn the principles of seed production of brinjal; Learn the principles of seed production of okra; Learn the principles of seed production of tomato; Field inspection at different stages of a crop and record of observations; Practice on seed extraction: dry method; Practice on seed extraction: fermentation method; Practice on seed extraction: chemical method; Practice on seed processing of vegetable crops

6. Cultivar purity and seed quality testing (0-3-0)

Practice

Learn the seed sampling methods; Physical Purity test; Moisture test; Germination test; Vigour test; Viability test; Varietal Identification through Grow out Test; Varietal Identification through Electrophoresis; Molecular test for seed quality assessment; Biochemical test for seed quality assessment; Control measures for stored grain disease and pest

Session Plan for the Entire Domain:

Theory:

1. Breeding methods: conventional and molecular approach (1-2-0)

Session 1.1: Classification of Plant Breeding methods

 Methods of Plant Breeding

Session 1.2: Genetic basis and breeding methods in self- pollinated crops

Breeding methods_ Self pollinated crops

Session 1.3: Hybridization techniques and handling of segregating population

Hybridization techniques & Handing of segregation populations

Session 1.4: Genetic basis and breeding methods of cross-pollinated crops

Breeding method_Back Cross

Session 1.5: Heterosis and inbreeding depression

Heterosis, inbreeding depression, various theories of Heterosis

Session 1.6: Hybrid breeding- steps /operations in production of hybrid varieties (Exploitation of heterosis)

Hybrid breeding- steps

Session 1.7: Male sterility and its application in hybrid seed production

Male Sterilitry

Application of Male Sterility in hybrid seed production

Session 1.8: Breeding methods in asexually propagated crops

Clonal Selection

Session 1.9: Special breeding techniques: Mutation breeding

Mutation Breeding

Mutation breeding procedure

Session 1.10: Special breeding techniques: Breeding for abiotic stresses

Breeding for Abiotic resistance

Breeding for abiotic_ Drought

Session 1.11: Special breeding techniques: Breeding for biotic stresses

Breeding for resistance to disease and insect pests

Breeding for Disease resistance

Breeding for Insect resistance

Session 1.12: DNA markers and its application in plant breeding

Biotechnological tools-DNA markers

Session 1.15: Release and notification system in India

Variety Development, Evaluation, Testing & Release in India

Session 1.16: Cultivar development-variety testing procedures, release and notification system in India and IPR

IPR

2. Seed production of vegetable and cereals crops (1-2-0)

Session 2.1: Importance and scope of vegetable seed production in India

Lec 1.pdf

Lec 1.ppt

Session 2.2: Planning for certified, foundation and breeder seed production

Lec 2

Session 2.3: Seed quality: Certification standards for self and cross pollinated and vegetatively propagated crops

Lec 3.pdf

Lec 3.ppt

 

 

Session 2.4: Principles of seed production in vegetable crops

Lec 4

Session 2.5: Seed production in varieties and hybrids of Paddy

Lec 5

Lec 5 (I)

Lec 5 (II)

Session 2.6: Seed production in varieties and hybrids of Maize

Lec 6

Lec 6 (I)

Lec 6 (II)

Lec 6 (III)

Session 2.7 - Session 2.9: Seed production in varieties and hybrids of Tomato, Brinjal and Okra

Lec 7

Lec 8

Lec 9

Lec 7-8

Lec 9 (I)

Lec 7-9

Session 2.10: Harvesting/picking stage and seed extraction in vegetables crops

Click here

Lec 10

Lec 10 (I)

Session 2.11 - Session 2.13: Seed drying, Seed processing, Seed storage

Lec 11

Lec 12.ppt

Lec 12.pdf

Lec 13 (I)

Lec 13 (II)

Session 2.14: Seed health and measures for pest and disease control during storage

Lec 14

Lec 14 (I)

Lec 14 (II)

Session 2.15: Seed Invigouration

Lec 15

Session 2.16: Seed Village

Lec 16

3. Seed certification (1-2-0)

Session 3.1: Seed policy 

Lec 1

Lec 1 (I)

Session 3.2: Seed Certification

Lec 2

Lec 2 (I)

Session 3.3: Seed Act

Lec 3

Lec 3 (I)

Session 3.4: Seed Control Order

Lec 4

Lec 4 (I)

Session 3.5: Indian Minimum Seed Certification Standards (I.M.S.C.S.)

Lec 5

Lec 5 (I)

Session 3.6: Phases, Planning and management of seed certification programmes

Lec 6

Session 3.7: Field Inspection: principles, phases and procedures, post-harvest inspection and evaluation

Lec 7

Session 3.8: Seed sampling

Lec 8

Session 3.9: Seed testing

Lec 9

Lec 9 (I)

Lec 9 (II)

Session 3.10: Seed Viability

Lec 10

Session 3.11: Varietal Identification

Lec 11

Lec 11 (I)

Session 3.12: Grant of certificate and certification fee and other service charges

Lec 12

Session 3.13: Seed marketing: structure and organization and pricing policy

Lec 13

Lec 13 (I)

Session 3.14: Role of WTO and OECD in seed marketing

Lec 14

Lec 14 (I)

Session 3.15: IPR

Lec 15

Session 3.16: PPV&FRA

Lec 16

Lec 16 (I)

Practice:

1. Breeding methods: conventional and molecular approach (0-3-0)

Session 1.1: Emasculation and hybridization techniques in Rice

Emasculation and pollinaton technique in rice

Session 1.2: Emasculation and hybridization techniques in maize

Emasculation and pollination technique in maize

Floral biology of maize

Session 1.3: Emasculation and hybridization techniques in vegetable crops: Tomato

Emasculation and pollination technique in tomato 

Session 1.4: Emasculation and hybridization techniques in vegetable crops: Okra

Emasculation and pollination technique in Okra

Session 1.5: Emasculation and hybridization techniques in vegetable crops: Brinjal

Pollination technique in eggplant 

Session 1.6: Handing of segregating populations

Handing of segregating populations

Session 1.7: Designs used in plant breeding experiment: RBD analysis

Designs used in plant breeding experiment_ RBD

Session 1.8: Estimation of heterosis

Estimation of heterosis

Session 1.9:  Inbreeding depression 

Inbreeding depression

Session 1.10: Heritability

Heritability

Session 1.11: Learning techniques in hybrid seed production using male-sterility in field crops 

Field techniques for Hybrid seed production

Session 1.12: Use of male sterility in hybrid seed production: Rice

Hybrid seed production in rice

Session 1.13: Genetic Mechanisms for Hybrid Seed Production in vegetable crops

Genetic Mechanisms for Hybrid Seed Production in veg crops

Session 1.14: Visit to AICRP plots to know about different trials for cultivar development

Session 1.15: DNA isolation

Plant genomic DNA isolation 

Session 1.16:  DNA purity 

Quantify DNA with a Spectrophotometer 

Session 1.17: DNA  quantification tests

Quantify DNA with a Spectrophotometer 

Session 1.18: DNA finger printing using PCR-based DNA markers 

DNA finger printing using RAPD marker 

cultivar identification in maize using SSR markers 

Session 1.20: Gel scoring  

Determining DNA Fragment Length in a Gel 

Session 1.21:  Data analysis for tagging 

Click here

Session 1.22: Phylogenetic relationship

 Click here

Session 1.23: Use of standard MAS procedures- screening crops for resistant to different biotic stress traits

Genomics based breeding research for improving resistance to biotic and abiotic stress in cereals 

Session 1.24: Use of standard MAS procedures- screening crops for resistant to different abiotic stress traits

Genomics based breeding research for improving resistance to biotic and abiotic stress in cereals 

Session 1.25: Breeding for quality traits

Click here

Session 1.26: Breeding for drought tolerant using MAS

Click here

Session 1.27: Breeding for salinity tolerant using MAS 

Click here

Session 1.28: Breeding for heat tolerant using MAS

Click here

Session 1.29: Breeding for cold tolerant using MAS

Breeding for cold tolerant

Session 1.30: Breeding for mineral toxicity using MAS

Mineral toxicity

Session 1.31: Release of new variety

Click here

Session 1.32: Software analysis for interpretation of molecular data

Click here

2. Seed production of vegetable and cereals crops (0-3-0)

Session 2.1 - Session 2.2: Selection of suitable areas/locations, seed/planting material for quality seed production

Click here

Lec 1

Session 2.3 - Session 2.4: Determination of planting ratios for hybrid seed production of cereals

Click here

Lec 2

Session 2.5- Session 2.6: Study of floral biology of Paddy and Maize

Click here

Click here

Lec 5

Lec 6

Session 2.7 - Session 2.9: Study of floral biology of Tomato, Brinjal and Okra

Click here

Click here

Click here

Lec 7

Lec 8

Lec 9

Session 2.10 - Session 2.11: Determination of planting ratios for hybrid seed production of vegetables

Click here

Lec 10-11

Session 2.12 - Session 2.14: Seed extraction methods and their effect on quality of vegetables

Click here

Click here

Click here

Lec 12-14

Session 2.15 - Session 2.17: Seed and seedling vigour test

Click here

Click here

Lec 15-17

Session 2.18 - Session 2.22: Practice on seed production of varieties and hybrids in cereal crops

Click here

Click here

Click here

Click here

Session 2.23 - Session 2.27: Practice on seed production of varieties and hybrids in vegetable crops

Click here

Click here

Click here

Click here

Session 2.28 - Session 2.30: Visit to Seed Production Farms

Session 2.31: Visit to Seed Testing Laboratory.

Session 2.32: Visit to Seed Processing Unit.

3. Seed certification (0-3-0)

Session 3.1 - Session 3.2: General procedure of seed certification

Click here

Click here

Lec 1 (I)

Lec 1 (II)

Session 3.3 - Session 3.4: Identification of weed and other crop seeds as per specific crops

Click here

Click here

Session 3.5 - Session 3.6: Field inspection at different stages of a crop and observations recorded

Click here

Click here

Session 3.7 - Session 3.8: Inspection and sampling at harvesting/threshing for seed law enforcement

Click here

Click here

Session 3.9 - Session 3.10: Inspection and sampling at processing and after processing for seed law enforcement

Click here

Click here

Session 3.11 - Session 3.13: Sampling methods and seed quality test

Click here

Click here

Click here

Session 3.14 - Session 3.15: Seed viability testing

Click here

Click here

Click here

Session 3.16 - Session 3.17: Seed germination

Click here

Click here

Session 3.18 - Session 3.19: Specifications for tags and labels to be used for certification purpose

Click here

Click here

Session 3.20 - Session 3.24: Varietal Identification through Grow Out Test and Electrophoresis

Click here

Click here

Lec 19-23

Lec 19-23 (I)

Session 3.25 - Session 3.28: Molecular and biochemical test for pre- and post-harvest quality control

Click here

Click here

Click here

Lec 25-28

Lec 25-28 (I)

Session 3.29: Visits to Seed Testing Laboratory

Session 3.30 - Session 3.31: Visit to private and public sector seed companies

Session 3.32: Visit to  certification agency

4. Hybridization techniques (0-3-0)

Session 4.1 - Session 4.10: Growing different genotypes of a crop using appropriate experimental design

Click here

Click here

Click here

Session 4.11 - Session 4.20: Practicing emasculation and pollination techniques in various crops.

Click here

Click here

Click here

Session 4.21 - Session 4.26: Harvesting of F1 seeds 

Click here

Session 4.27 - Session 4.35: DNA isolation of F1 progenies

Click here

Click here

Session 4.36 - Session 4.40: Check the hybrid (F1) fidelity using molecular markers

Click here

Click here

Session 4.41 - Session 4.42: Visit to research station to learn techniques in hybrid seed production using male-sterility in field crops

Session 4.43 - Session 4.46: Visit to hybrid seed production plots

Session 4.47 - Session 4.48: Visit to research station to understand MAS procedures

5. Seed production of vegetable crops (0-3-0)

Session 5.1 - Session 5.8: Selection of suitable areas/locations for high quality seed/planting material production

Production of Quality Seed

Session 5.9 - Session 5.16: Learn the principles of seed production of brinjal

Click here

Brinjal

Session 5.17 - Session 5.25: Learn the principles of seed production of okra

Okra

Seed Production of Okra

Click here

Session 5.26 - Session 5.30: Learn the principles of seed production of tomato

Tomato

Tomato

Click here

Session 5.31 - Session 5.35: Field inspection at different stages of a crop and record of observations

Click here

Click here

Session 5.36 - Session 5.39: Practice on seed extraction: dry method

Seed Extraction

Click here

Session 5.40 - Session 5.42: Practice on seed extraction: fermentation method

Seed Extraction

Seed Extraction (I)

Click here

Session 5.43 - Session 5.45: Practice on seed extraction: chemical method

Click here

Seed Extraction

Session 5.46 - Session 5.48: Practice on seed processing of vegetable crops

Click here

Seed Processing (I)

Seed Processing

6. Cultivar purity and seed quality testing (0-3-0)

Session 6.1 - Session 6.5: Learn the seed sampling methods

Click here

Click here

Click here

Session 6.6 - Session 6.10: Seed testing: physical purity

Click here

Click here

Click here

Session 6.11 - Session 6.14: Seed testing: moisture test

Click here

Click here

Click here

Session 6.15 - Session 6.19: Seed testing: germination and vigour test

Click here

Click here

Click here

Seed Germination

Session 6.20 - Session 6.25: Seed testing: viability test

Click here

Click here

Tz Test

Session 6.26 - Session 6.30: Varietal Identification through Grow Out Test

Click here

Session 6.31 - Session 6.35: Varietal Identification through Electrophoresis

Click here

Click here

Session 6.36 - Session 6.43: Molecular and Biochemical test for quality assessment

Varietal Identification and GMO

Varietal Identification

Session 6.44 - Session 6.48: Control measures for stored grain disease and pest

Click here

Click here

Seed Storage PPT

Seed Storage

7. Practices in Units/Field/Lab/Project (0-0-11)

To acquire real-time experience in addition to this practice work in AELP, students will be tied up with

  • Research Institute

  • Seed Production Farms

  • Seed Testing Laboratory

  • Seed Processing Unit

 

 

E-Materials

Breeding Methods - Conventional and Molecular Approach

Seed Production of Vegetable and Cereal Crops

Seed Certification

Our Main Teachers

I have completed my Ph.D. in Seed Science and Technology from Bidhan Chandra Krishi Viswavidyalaya, Mohanpur. I received University Research Fellowship during the tenure of my Doctoral research. My doctoral topic was “Pre-sowing Seed Priming with Ag-Nanoparticle in Green gram: Chemo-morphic Changes during Seed Development and Response Pattern for Seed Production”. I have completed my […]

Dr. Vinay Kumar

Associate Professor
VIEW PROFILE

I am a Plant breeder, Now working on application of nano particles in  post harvest management of horticultural crops, mainly Onion. I along with co-workers reported first time worldwide a colorimetric sensor for visual detection of post harvest spoilage of onions. I have keen interest in horticultural crops and its value addition.